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Communication

Spectral-Phase Interferometry Detection of Ochratoxin A via Aptamer-Functionalized Graphene Coated Glass

1
National Research University of Electronic Technology, 124498 Moscow, Russia
2
Moscow Institute of Physics and Technology, 9 Institutskii per., Dolgoprudny, 141700 Moscow, Russia
3
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St, 119991 Moscow, Russia
4
BioSense Institute-Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, 21000 Novi Sad, Serbia
5
Graphenea, Avenida de Tolosa 76, 20018 Donostia-San Sebastián, Spain
*
Author to whom correspondence should be addressed.
Nanomaterials 2021, 11(1), 226; https://doi.org/10.3390/nano11010226
Received: 12 December 2020 / Revised: 8 January 2021 / Accepted: 12 January 2021 / Published: 16 January 2021
In this work, we report a novel method of label-free detection of small molecules based on direct observation of interferometric signal change in graphene-modified glasses. The interferometric sensor chips are fabricated via a conventional wet transfer method of CVD-grown graphene onto the glass coverslips, lowering the device cost and allowing for upscaling the sensor fabrication. For the first time, we report the use of graphene functionalized by the aptamer as the bioreceptor, in conjunction with Spectral-Phase Interferometry (SPI) for detection of ochratoxin A (OTA). In a direct assay with an OTA-specific aptamer, we demonstrated a quick and significant change of the optical signal in response to the maximum tolerable level of OTA concentration. The sensor regeneration is possible in urea solution. The developed platform enables a direct method of kinetic analysis of small molecules using a low-cost optical chip with a graphene-aptamer sensing layer. View Full-Text
Keywords: CVD graphene; label-free biosensing; mycotoxins; aptamer; spectral-phase interferometry CVD graphene; label-free biosensing; mycotoxins; aptamer; spectral-phase interferometry
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MDPI and ACS Style

Nekrasov, N.; Yakunina, N.; Pushkarev, A.V.; Orlov, A.V.; Gadjanski, I.; Pesquera, A.; Centeno, A.; Zurutuza, A.; Nikitin, P.I.; Bobrinetskiy, I. Spectral-Phase Interferometry Detection of Ochratoxin A via Aptamer-Functionalized Graphene Coated Glass. Nanomaterials 2021, 11, 226. https://doi.org/10.3390/nano11010226

AMA Style

Nekrasov N, Yakunina N, Pushkarev AV, Orlov AV, Gadjanski I, Pesquera A, Centeno A, Zurutuza A, Nikitin PI, Bobrinetskiy I. Spectral-Phase Interferometry Detection of Ochratoxin A via Aptamer-Functionalized Graphene Coated Glass. Nanomaterials. 2021; 11(1):226. https://doi.org/10.3390/nano11010226

Chicago/Turabian Style

Nekrasov, Nikita, Natalya Yakunina, Averyan V. Pushkarev, Alexey V. Orlov, Ivana Gadjanski, Amaia Pesquera, Alba Centeno, Amaia Zurutuza, Petr I. Nikitin, and Ivan Bobrinetskiy. 2021. "Spectral-Phase Interferometry Detection of Ochratoxin A via Aptamer-Functionalized Graphene Coated Glass" Nanomaterials 11, no. 1: 226. https://doi.org/10.3390/nano11010226

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