Next Article in Journal
Synthesis and Cytotoxicity Studies of Wood-Based Cationic Cellulose Nanocrystals as Potential Immunomodulators
Previous Article in Journal
Nanocomposites of NiO/CuO Based MOF with rGO: An Efficient and Robust Electrocatalyst for Methanol Oxidation Reaction in DMFC
Previous Article in Special Issue
Proinflammatory Effect of Carbon-Based Nanomaterials: In Vitro Study on Stimulation of Inflammasome NLRP3 via Destabilisation of Lysosomes
Open AccessArticle

Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes

1
Department of Life Sciences, University of Trieste, 34127 Trieste, Italy
2
CNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR 3572, University of Strasbourg, ISIS, 67000 Strasbourg, France
3
Department of Chemical and Pharmaceutical Sciences, University of Trieste, 34127 Trieste, Italy
4
Fondazione Istituto di Ricerca Pediatrica, Città della Speranza, 35100 Padua, Italy
5
Graphenea, 20009 Donostia-San Sebastián, Spain
6
Facultad de Ciencias y Tecnologías Químicas, Universidad de Castilla-La Mancha (UCLM), 13071 Ciudad Real, Spain
7
Instituto Regional de Investigacion Científica Aplicada (IRICA), Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain
8
Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 182, 20014 Donostia San Sebastián, Spain
9
Basque Foundation for Science (IKERBASQUE), 48013 Bilbao, Spain
*
Authors to whom correspondence should be addressed.
Nanomaterials 2020, 10(8), 1602; https://doi.org/10.3390/nano10081602
Received: 3 August 2020 / Revised: 11 August 2020 / Accepted: 12 August 2020 / Published: 15 August 2020
In the frame of graphene-based material (GBM) hazard characterization, particular attention should be given to the cutaneous effects. Hence, this study investigates if HaCaT skin keratinocytes exposed to high concentrations of few-layer graphene (FLG) or partially dehydrated graphene oxide (d-GO) for a short time can recover from the cytotoxic insult, measured by means of cell viability, mitochondrial damage and oxidative stress, after GBM removal from the cell medium. When compared to 24 or 72 h continuous exposure, recovery experiments suggest that the cytotoxicity induced by 24 h exposure to GBM is only partially recovered after 48 h culture in GBM-free medium. This partial recovery, higher for FLG as compared to GO, is not mediated by autophagy and could be the consequence of GBM internalization into cells. The ability of GBMs to be internalized inside keratinocytes together with the partial reversibility of the cellular damage is important in assessing the risk associated with skin exposure to GBM-containing devices. View Full-Text
Keywords: carbon nanomaterials; 2D materials; autophagy; biocompatibility; dermal toxicity carbon nanomaterials; 2D materials; autophagy; biocompatibility; dermal toxicity
Show Figures

Figure 1

MDPI and ACS Style

Pelin, M.; Lin, H.; Gazzi, A.; Sosa, S.; Ponti, C.; Ortega, A.; Zurutuza, A.; Vázquez, E.; Prato, M.; Tubaro, A.; Bianco, A. Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes. Nanomaterials 2020, 10, 1602. https://doi.org/10.3390/nano10081602

AMA Style

Pelin M, Lin H, Gazzi A, Sosa S, Ponti C, Ortega A, Zurutuza A, Vázquez E, Prato M, Tubaro A, Bianco A. Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes. Nanomaterials. 2020; 10(8):1602. https://doi.org/10.3390/nano10081602

Chicago/Turabian Style

Pelin, Marco; Lin, Hazel; Gazzi, Arianna; Sosa, Silvio; Ponti, Cristina; Ortega, Amaya; Zurutuza, Amaia; Vázquez, Ester; Prato, Maurizio; Tubaro, Aurelia; Bianco, Alberto. 2020. "Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes" Nanomaterials 10, no. 8: 1602. https://doi.org/10.3390/nano10081602

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop