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Article

A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol

1
Department of Chemical Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia
2
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia
3
Polymer Research Centre, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia
*
Authors to whom correspondence should be addressed.
Nanomaterials 2020, 10(7), 1346; https://doi.org/10.3390/nano10071346
Received: 14 April 2020 / Revised: 18 May 2020 / Accepted: 19 May 2020 / Published: 10 July 2020
A simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilization of 76-mer aptamer probe. The morphology and structure of the nanomaterial were characterized by UV-visible absorption spectra, Fluorescence spectra, Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Moreover, cyclic voltammetry and electrochemical impedance spectroscopy were used to investigate the electrochemical performance of the prepared electrodes. Subsequently, impedimetric (EIS) measurements were employed to investigate the relative impedances changes before and after E2 binding, which results in a linear relationship of E2 concentration in the range of 1.0 × 10−7 to 1.0 × 10 −12 M, with a detection limit of 0.5 × 10−12 M. Moreover, the developed biosensor showed high selectivity toward E2 and exhibited excellent discrimination against progesterone (PRG), estriol (E3) and bisphenol A (BPA), respectively. Moreover, the average recovery rate of spiked river water samples with E2 ranged from 98.2% to 103.8%, with relative standard deviations between 1.1% and 3.8%, revealing the potential application of the present biosensor for E2 detection in water samples. View Full-Text
Keywords: carbon nanodots; impedance analysis; aptamer; 17ß-estradiol; aptasensor carbon nanodots; impedance analysis; aptamer; 17ß-estradiol; aptasensor
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MDPI and ACS Style

Mat Zaid, M.H.; Abdullah, J.; Rozi, N.; Mohamad Rozlan, A.A.; Abu Hanifah, S. A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol. Nanomaterials 2020, 10, 1346. https://doi.org/10.3390/nano10071346

AMA Style

Mat Zaid MH, Abdullah J, Rozi N, Mohamad Rozlan AA, Abu Hanifah S. A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol. Nanomaterials. 2020; 10(7):1346. https://doi.org/10.3390/nano10071346

Chicago/Turabian Style

Mat Zaid, Mohd H., Jaafar Abdullah, Normazida Rozi, Aliff A. Mohamad Rozlan, and Sharina Abu Hanifah. 2020. "A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol" Nanomaterials 10, no. 7: 1346. https://doi.org/10.3390/nano10071346

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