Next Article in Journal
Effect of Chemical Solvents on the Wetting Behavior Over Time of Femtosecond Laser Structured Ti6Al4V Surfaces
Next Article in Special Issue
Gated Resonance Energy Transfer (gRET) Controlled by Programmed Death Protein Ligand 1
Previous Article in Journal
Phonon Bridge Effect in Superlattices of Thermoelectric TiNiSn/HfNiSn With Controlled Interface Intermixing
Previous Article in Special Issue
Highly Sensitive and Selective Colorimetric Detection of Creatinine Based on Synergistic Effect of PEG/Hg2+–AuNPs
Article

Detection of C-Reactive Protein Using Histag-HRP Functionalized Nanoconjugate with Signal Amplified Immunoassay

School of Mechanical Engineering, Korea University of Technology and Education, Cheonan 31253, Korea
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(6), 1240; https://doi.org/10.3390/nano10061240
Received: 5 June 2020 / Revised: 22 June 2020 / Accepted: 23 June 2020 / Published: 26 June 2020
(This article belongs to the Special Issue Gold Nanoparticle-Based Biosensors)
Ultrasensitive detection of biomarkers is highly significant for disease prognosis and public health treatment. Despite wide acceptance in routine laboratory tests, the conventional enzyme-linked immunosorbent assay (ELISA) has been of limited use for early biomarker detection due to insufficient sensitivity and multiple long incubation time. Several nanoprobes have been introduced to circumvent the limitation, however, rapid, simple, and chemical-free nanoprobe synthesis and sensitive detection methods, particularly for ELISA, are still lacking. In this study, we have synthesized a gold nanoprobe, conjugated with multiple 6X-histidine (6X-his) peptide and nickel-horseradish peroxidase (Ni2+-HRP), for enhancing the colorimetric signal in ELISA. The developed nanoprobe has been tested for the detection of immunologically significant C-reactive protein (CRP) in ELISA format. The performance of designed probe is validated by testing standard and serum samples, and the detection limit of 32.0 pg/mL with R2 = 0.98 is confirmed. Furthermore, a comparative analysis of the developed nanoprobe was performed with ELISA developed on conventional guidelines, the proposed immunoassay showed an increase of 12-fold sensitivity for detecting CRP due to the high loading of 6Xhis peptide and binding of multiple Ni2+-HRP on a gold nanoparticle. Additionally, the proposed assay provides a simple, fast, and cost-efficient (not requiring multiple antibodies) detection of CRP with easy nanoprobe synthesis. Moreover, the developed Histag-HRP functionalized nanoconjugate immunoassay is flexible and can be applied to other biomarkers efficiently by using disease specific antibody. View Full-Text
Keywords: Histag-HRP nanoconjugate; immunoassay; C-reactive protein; ELISA; biosensor Histag-HRP nanoconjugate; immunoassay; C-reactive protein; ELISA; biosensor
Show Figures

Graphical abstract

MDPI and ACS Style

Siddiqui, M.F.; Khan, Z.A.; Park, S. Detection of C-Reactive Protein Using Histag-HRP Functionalized Nanoconjugate with Signal Amplified Immunoassay. Nanomaterials 2020, 10, 1240. https://doi.org/10.3390/nano10061240

AMA Style

Siddiqui MF, Khan ZA, Park S. Detection of C-Reactive Protein Using Histag-HRP Functionalized Nanoconjugate with Signal Amplified Immunoassay. Nanomaterials. 2020; 10(6):1240. https://doi.org/10.3390/nano10061240

Chicago/Turabian Style

Siddiqui, Mohd F., Zeeshan A. Khan, and Seungkyung Park. 2020. "Detection of C-Reactive Protein Using Histag-HRP Functionalized Nanoconjugate with Signal Amplified Immunoassay" Nanomaterials 10, no. 6: 1240. https://doi.org/10.3390/nano10061240

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop