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Proteomic Analysis Identifies Markers of Exposure to Cadmium Sulphide Quantum Dots (CdS QDs)

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Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43123 Parma, Italy
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Royal Institute of Technology (KTH), Department of Chemistry, Division of Glycoscience, School of Engineering Sciences in Chemistry, Biotechnology and Health, AlbaNova University Center, SE-106 91 Stockholm, Sweden
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ARC Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, The University of Adelaide, Urbrae, SA 5064, Australia
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Department of Nanomaterials, Institute of Materials for Electronics and Magnetism (IMEM)Department of Nanomaterials, National Research Council (CNR), 43124 Parma, Italy
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The Italian National Interuniversity Consortium for Environmental Sciences (CINSA), 43124 Parma, Italy
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(6), 1214; https://doi.org/10.3390/nano10061214
Received: 6 April 2020 / Revised: 10 June 2020 / Accepted: 17 June 2020 / Published: 22 June 2020
(This article belongs to the Section Biology and Medicines)
The use of cadmium sulphide quantum dot (CdS QD)-enabled products has become increasingly widespread. The prospect of their release in the environment is raising concerns. Here we have used the yeast model Saccharomyces cerevisiae to determine the potential impact of CdS QD nanoparticles on living organisms. Proteomic analyses and cell viability assays performed after 9 h exposure revealed expression of proteins involved in oxidative stress and reduced lethality, respectively, whereas oxidative stress declined, and lethality increased after 24 h incubation in the presence of CdS QDs. Quantitative proteomics using the iTRAQ approach (isobaric tags for relative and absolute quantitation) revealed that key proteins involved in essential biological pathways were differentially regulated over the time course of the experiment. At 9 h, most of the glycolytic functions increased, and the abundance of the number of heat shock proteins increased. This contrasts with the situation at 24 h where glycolytic functions, some heat shock proteins as well as oxidative phosphorylation and ATP synthesis were down-regulated. It can be concluded from our data that cell exposure to CdS QDs provokes a metabolic shift from respiration to fermentation, comparable to the situation reported in some cancer cell lines. View Full-Text
Keywords: baker’s yeast; proteomics; iTRAQ; engineered nanomaterials; quantum dots; glycolysis; oxidative phosphorylation; endoplasmic reticulum baker’s yeast; proteomics; iTRAQ; engineered nanomaterials; quantum dots; glycolysis; oxidative phosphorylation; endoplasmic reticulum
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MDPI and ACS Style

Gallo, V.; Srivastava, V.; Bulone, V.; Zappettini, A.; Villani, M.; Marmiroli, N.; Marmiroli, M. Proteomic Analysis Identifies Markers of Exposure to Cadmium Sulphide Quantum Dots (CdS QDs). Nanomaterials 2020, 10, 1214. https://doi.org/10.3390/nano10061214

AMA Style

Gallo V, Srivastava V, Bulone V, Zappettini A, Villani M, Marmiroli N, Marmiroli M. Proteomic Analysis Identifies Markers of Exposure to Cadmium Sulphide Quantum Dots (CdS QDs). Nanomaterials. 2020; 10(6):1214. https://doi.org/10.3390/nano10061214

Chicago/Turabian Style

Gallo, Valentina, Vaibhav Srivastava, Vincent Bulone, Andrea Zappettini, Marco Villani, Nelson Marmiroli, and Marta Marmiroli. 2020. "Proteomic Analysis Identifies Markers of Exposure to Cadmium Sulphide Quantum Dots (CdS QDs)" Nanomaterials 10, no. 6: 1214. https://doi.org/10.3390/nano10061214

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