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Open AccessArticle

Solid Plasmonic Substrates for Breast Cancer Detection by Means of SERS Analysis of Blood Plasma

1
Faculty of Physics, “Babeș-Bolyai” University, M. Kogalniceanu 1, 400084 Cluj-Napoca, Romania
2
MedFuture Research Center for Advance Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, L. Pasteur 4-6, 400349 Cluj-Napoca, Romania
3
Department of Senology, The Oncology Institute “Prof. Dr. Ion Chiricuta”, 34-36 Republicii Street, 400015 Cluj-Napoca, Romania
4
Department of Dental Propedeutics and Esthetics, “Iuliu Hațieganu” University of Medicine and Pharmacy, 8 V. Babes Street, 400012 Cluj-Napoca, Romania
5
Department of Chemistry, Wright State University, 3640 Colonel Glenn Hwy., Dayton, OH 45435-0001, USA
6
Department of Pharmaceutical Physics-Biophysics, “Iuliu Hațieganu” University of Medicine and Pharmacy, L. Pasteur 6, 400349 Cluj-Napoca, Romania
*
Authors to whom correspondence should be addressed.
These authors equally contributed to this paper.
Nanomaterials 2020, 10(6), 1212; https://doi.org/10.3390/nano10061212
Received: 11 May 2020 / Revised: 10 June 2020 / Accepted: 17 June 2020 / Published: 21 June 2020
(This article belongs to the Special Issue Application of Nanoscale Materials for Cancer Diagnostic and Therapy)
Surface enhanced Raman spectroscopy (SERS) represents a promising technique in providing specific molecular information that could have a major impact in biomedical applications, such as early cancer detection. SERS requires the presence of a suitable plasmonic substrate that can generate enhanced and reproducible diagnostic relevant spectra. In this paper, we propose a new approach for the synthesis of such a substrate, by using concentrated silver nanoparticles purified using the Tangential Flow Filtration method. The capacity of our substrates to generate reproducible and enhanced Raman signals, in a manner that can allow cancer detection by means of Multivariate Analysis (MVA) of Surface Enhanced Raman (SER) spectra, has been tested on blood plasma samples collected from 35 healthy donors and 29 breast cancer patients. All the spectra were analyzed by a combined Principal Component-Linear Discriminant Analysis. Our results facilitated the discrimination between healthy donors and breast cancer patients with 90% sensitivity, 89% specificity and 89% accuracy. This is a direct consequence of substrates’ ability to generate diagnostic relevant spectral information by performing SERS measurements on pristine blood plasma samples. Our results suggest that this type of solid substrate could be employed for the detection of other types of cancer or other diseases by means of MVA-SERS procedure. View Full-Text
Keywords: SERS; solid plasmonic substrates; biofluids; MVA-SERS based cancer detection SERS; solid plasmonic substrates; biofluids; MVA-SERS based cancer detection
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MDPI and ACS Style

Știufiuc, G.F.; Toma, V.; Buse, M.; Mărginean, R.; Morar-Bolba, G.; Culic, B.; Tetean, R.; Leopold, N.; Pavel, I.; Lucaciu, C.M.; Știufiuc, R.I. Solid Plasmonic Substrates for Breast Cancer Detection by Means of SERS Analysis of Blood Plasma. Nanomaterials 2020, 10, 1212.

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