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Open AccessArticle

Detection of Aflatoxin B1 Based on a Porous Anodized Aluminum Membrane Combined with Surface-Enhanced Raman Scattering Spectroscopy

by 1, 2,3, 1, 2,4,*, 1,2,4, 1,2,4 and 2,3,4,*
1
College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China
2
Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, China
3
School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China
4
Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524088, China
*
Authors to whom correspondence should be addressed.
Nanomaterials 2020, 10(5), 1000; https://doi.org/10.3390/nano10051000
Received: 7 May 2020 / Revised: 21 May 2020 / Accepted: 21 May 2020 / Published: 24 May 2020
An Aflatoxin B1 (AFB1) biosensor was fabricated via an Ag nanoparticles assembly on the surface of a porous anodized aluminum (PAA) membrane. First, the Raman reporter 4-Aminothiophenol (4-ATP) and DNA (partially complementary to AFB1 aptamer) were attached to the surface of Ag nanoparticles (AgNPs) by chemical bonding to form a 4-ATP-AgNPs-DNA complex. Similarly, the surface of a PAA membrane was functionalized with an AFB1 aptamer. Then, the PAA surface was functionalized with 4-ATP-AgNPs-DNA through base complementary pairing to form AgNPs-PAA sensor with a strong Raman signal. When AFB1 was added, AgNPs would be detached from the PAA surface because of the specific binding between AFB1 and the aptamer, resulting in a reduction in Raman signals. The detection limit of the proposed biosensor is 0.009 ng/mL in actual walnut and the linear range is 0.01–10 ng/mL. The sensor has good selectivity and repeatability; it can be applied to the rapid qualitative and quantitative detection of AFB1. View Full-Text
Keywords: surface-enhanced Raman scattering (SERS); Ag nanoparticles; porous anodized aluminum membrane; Aflatoxin B1 surface-enhanced Raman scattering (SERS); Ag nanoparticles; porous anodized aluminum membrane; Aflatoxin B1
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MDPI and ACS Style

Feng, Y.; He, L.; Wang, L.; Mo, R.; Zhou, C.; Hong, P.; Li, C. Detection of Aflatoxin B1 Based on a Porous Anodized Aluminum Membrane Combined with Surface-Enhanced Raman Scattering Spectroscopy. Nanomaterials 2020, 10, 1000. https://doi.org/10.3390/nano10051000

AMA Style

Feng Y, He L, Wang L, Mo R, Zhou C, Hong P, Li C. Detection of Aflatoxin B1 Based on a Porous Anodized Aluminum Membrane Combined with Surface-Enhanced Raman Scattering Spectroscopy. Nanomaterials. 2020; 10(5):1000. https://doi.org/10.3390/nano10051000

Chicago/Turabian Style

Feng, Yanting; He, Lei; Wang, Ling; Mo, Rijian; Zhou, Chunxia; Hong, Pengzhi; Li, Chengyong. 2020. "Detection of Aflatoxin B1 Based on a Porous Anodized Aluminum Membrane Combined with Surface-Enhanced Raman Scattering Spectroscopy" Nanomaterials 10, no. 5: 1000. https://doi.org/10.3390/nano10051000

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