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Open AccessArticle

Test-System for Bacteria Sensing Based on Peroxidase-Like Activity of Inkjet-Printed Magnetite Nanoparticles

Laboratory of Solution Chemistry of Advanced Materials and Technologies, ITMO University, 197101 St. Petersburg, Russia
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Nanomaterials 2020, 10(2), 313; https://doi.org/10.3390/nano10020313
Received: 7 January 2020 / Revised: 5 February 2020 / Accepted: 8 February 2020 / Published: 12 February 2020
(This article belongs to the Section Synthesis, Interfaces and Nanostructures)
Rapid detection of bacterial contamination is an essential task in numerous medical and technical processes and one of the most rapidly developing areas of nano-based analytics. Here, we present a simple-to-use and special-equipment-free test-system for bacteria detection based on magnetite nanoparticle arrays. The system is based on peroxide oxidation of chromogenic substrate catalyzed by magnetite nanoparticles, and the process undergoes computer-aided visual analysis. The nanoparticles used had a pristine surface free of adsorbed molecules and demonstrated high catalytic activities up to 6585 U/mg. The catalytic process showed the Michaelis–Menten kinetic with Km valued 1.22 mmol/L and Vmax of 4.39 µmol/s. The nanoparticles synthesized were used for the creation of inkjet printing inks and the design of sensor arrays by soft lithography. The printed sensors require no special equipment for data reading and showed a linear response for the detection of model bacteria in the range of 104–108 colony-forming units (CFU) per milliliter with the detection limit of 3.2 × 103 CFU/mL.
Keywords: magnetite; nanoparticles; enzyme-like activity; bacteria detection; inkjet magnetite; nanoparticles; enzyme-like activity; bacteria detection; inkjet
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MDPI and ACS Style

Zakharzhevskii, M.; Drozdov, A.S.; Kolchanov, D.S.; Shkodenko, L.; Vinogradov, V.V. Test-System for Bacteria Sensing Based on Peroxidase-Like Activity of Inkjet-Printed Magnetite Nanoparticles. Nanomaterials 2020, 10, 313.

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