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Open AccessArticle

Magnetic Immobilization of Pichia pastoris Cells for the Production of Recombinant Human Serum Albumin

1
Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2
Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
3
School of Engineering, Faculty of Science and Engineering, the University of Waikato, Hamilton 3240, New Zealand
*
Authors to whom correspondence should be addressed.
Nanomaterials 2020, 10(1), 111; https://doi.org/10.3390/nano10010111
Received: 29 November 2019 / Revised: 22 December 2019 / Accepted: 3 January 2020 / Published: 6 January 2020
Magnetic immobilization as a novel technique was used to immobilize recombinant Pichia pastoris (GS115 Albumin) cells to produce human serum albumin (HSA). In this regard, magnetic nanoparticles (MNPs) coated with amino propyl triethoxy silane (APTES) were synthesized. P. pastoris cells were decorated with MNPs via nonspecific interactions. Decorated cells were magneto-responsible and easily harvested by applying an external magnetic field. The efficiency of magnetic immobilization (Ei) for cell removal was in direct relation with the MNP concentration and time of exposure to the magnetic field. By increasing the nanoparticles concentration, cells were harvested in a shorter period. Complete cell removal (Ei ≈ 100) was achieved in ≥0.5 mg/mL of MNPs in just 30 s. HSA is produced in an extremely high cell density (OD ~20) and it is the first time that magnetic immobilization was successfully employed for harvesting such a thick cell suspension. After 5 days of induction the cells, which were immobilized with 0.25 to 1 mg/mL of nanoparticles, showed an increased potency for recombinant HSA production. The largest increase in HSA production (38.1%) was achieved in the cells that were immobilized with 0.5 mg/mL of nanoparticles. These results can be considered as a novel approach for further developments in the P. pastoris-based system.
Keywords: cell immobilization; cell harvesting; magnetite (Fesub>3O4) nanoparticles; magnetic separation; recombinant protein production cell immobilization; cell harvesting; magnetite (Fesub>3O4) nanoparticles; magnetic separation; recombinant protein production
MDPI and ACS Style

Taghizadeh, S.-M.; Ebrahiminezhad, A.; Ghoshoon, M.B.; Dehshahri, A.; Berenjian, A.; Ghasemi, Y. Magnetic Immobilization of Pichia pastoris Cells for the Production of Recombinant Human Serum Albumin. Nanomaterials 2020, 10, 111.

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