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Open AccessArticle

A Three-Dimensional Dense Collagen Hydrogel to Model Cancer Cell/Osteoblast Interactions

1
Department of Mining and Materials Engineering, McGill University, Montréal, QC H3A 0C5, Canada
2
Departments of Medicine, Biochemistry and Anatomy & Cell Biology, McGill University, Montréal, QC H3A 0C7, Canada
3
Rosalind and Morris Goodman Cancer Research Centre, McGill University, Montréal, QC H3A 1A3, Canada
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
J. Funct. Biomater. 2018, 9(4), 72; https://doi.org/10.3390/jfb9040072
Received: 31 October 2018 / Revised: 26 November 2018 / Accepted: 3 December 2018 / Published: 12 December 2018
No curative treatment options exist once breast cancer metastasizes to bone. This is due, in part, to an incomplete understanding of how osteolytic cancers interact with bone. Presented here is a novel approach to study the interactions between triple negative breast cancer cells and osteoblasts within a 3D collagenous environment. More specifically, a dense collagen hydrogel was employed to model interactions between MDA-MB-231 breast cancer cells and MC3T3-E1 pre-osteoblasts. Co-cultures with these two cell types, or MDA-MB-231-derived conditioned medium applied to MC3T3-E1 cells, were established in the context of plastically compressed dense collagen gel matrices. Importantly, breast cancer-derived conditioned medium or the establishment of breast cancer/osteoblast co-cultures did not negatively influence MC3T3-E1 cell viability. The inclusion of either conditioned medium or the presence of MDA-MB-231 cells resulted in impaired MC3T3-E1 differentiation into osteoblasts, which coincided with reduced osteoblast-mediated mineralization. The results presented here demonstrate that dense collagen gels provide a model environment to examine the effect of osteolytic breast cancer cells on osteoblast differentiation and subsequent mineralization of the collagen scaffold. View Full-Text
Keywords: breast cancer; osteoblasts; bone metastasis; dense collagen hydrogel; mineralization breast cancer; osteoblasts; bone metastasis; dense collagen hydrogel; mineralization
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MDPI and ACS Style

James-Bhasin, M.; Siegel, P.M.; Nazhat, S.N. A Three-Dimensional Dense Collagen Hydrogel to Model Cancer Cell/Osteoblast Interactions. J. Funct. Biomater. 2018, 9, 72.

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