Specimen Shape and Elution Time Affect the Mineralization and Differentiation Potential of Dental Pulp Stem Cells to Biodentine
Abstract
:1. Introduction
2. Materials and Methods
2.1. Material and Elution Preparation
2.2. pH Measurement of Leachate
2.3. Calcium Release
2.4. Cell Culture and Characterization
2.5. Cell Proliferation and Differentiation
2.6. Statistical Analysis
3. Results
3.1. pH Variation, Calcium Release and Cell Proliferation
3.2. Mineralization Potential and Gene Expression
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer | Sequence |
---|---|
MEPE Forward | GGCCAGTGACTGCGATTAAAC |
MEPE Reverse | CCTTCGAGTGTGCTTTAGCAT |
DMP-1 Forward | CTCCGAGTTGGACGATGAGG |
DMP-1 Reverse | TCATGCCTGCACTGTTCATTC |
DSPP Forward | TGGCGATGCAGGTCACAAT |
DSPP Reverse | CCATTCCCACTAGGACTCCCA |
RUNX2 Forward | CACTGGCGCTGCAACAAGA |
RUNX2 Reverse | CATTCCGGAGCTCAGCAGAATAA |
COL I Forward | CTGACCTTCCTGCGCCTGATGTCC |
COL I Reverse | GTCTGGGGCACCAACGTCCAAGGG |
OCN Forward | ATGAGAGCCCTCAGACTCCTC |
OCN Reverse | CGGGCCGTAGAAGCGCCGATA |
D10 | D50 | D90 |
---|---|---|
2.79 ± 0.04 | 9.73 ± 0.95 | 46.82 ± 6.71 |
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Phang, V.; Malhotra, R.; Chen, N.N.; Min, K.-S.; Yu, V.S.H.; Rosa, V.; Dubey, N. Specimen Shape and Elution Time Affect the Mineralization and Differentiation Potential of Dental Pulp Stem Cells to Biodentine. J. Funct. Biomater. 2024, 15, 1. https://doi.org/10.3390/jfb15010001
Phang V, Malhotra R, Chen NN, Min K-S, Yu VSH, Rosa V, Dubey N. Specimen Shape and Elution Time Affect the Mineralization and Differentiation Potential of Dental Pulp Stem Cells to Biodentine. Journal of Functional Biomaterials. 2024; 15(1):1. https://doi.org/10.3390/jfb15010001
Chicago/Turabian StylePhang, Valene, Ritika Malhotra, Nah Nah Chen, Kyung-San Min, Victoria Soo Hoon Yu, Vinicius Rosa, and Nileshkumar Dubey. 2024. "Specimen Shape and Elution Time Affect the Mineralization and Differentiation Potential of Dental Pulp Stem Cells to Biodentine" Journal of Functional Biomaterials 15, no. 1: 1. https://doi.org/10.3390/jfb15010001