3. Results
The final data set contained subjective and physiological data of 56 participants. Three datasets had to be excluded due to incompleteness. Data for intention, descriptive norms, and PTTF were not normally distributed and transformation did not effectuate normal distribution, i.e., log, log+1, Tukey’s Ladder of Powers, Cube root nor square root transformation. There were no significant differences between the demographic characteristics or initial motivational state of the two groups, e.g., attitudes, intention, subjective norms and descriptive norms.
As a persuasion check, a within-between MANOVA with Bonferroni–Holm correction was conducted to compare the main effects of condition and time, and the interaction effect between manipulation and time on attitudes and intentions towards limited meat consumption. Both the main and the interaction effects were not significant; attitudes and intentions did not change over the course of the experiment in either condition.
As a manipulation check, an independent sample one-tailed Mann–Whitney U t-test with Bonferroni–Holm correction revealed that the HCL condition indeed evoked significantly more anger and perceived threat to freedom than the LCL persuasive messages (
Table 3).
The results of multiple linear mixed models with experiment segment and manipulation condition as fixed effects and subject as random effect showed physiological reactivity differed significantly between experimental segments for HR, SDNN, and RMSSD (
Table 4). On average, SDNN and RMSSD were 89 and 36 ms lower, and HR 1.30 bpm higher during the persuasive messages compared to the short baseline. During the factual video, HR reactivity was 1.03 bpm lower than during the persuasive messages, whereas SDNN reactivity was 54 ms higher (see also
Figure 2). With the exception of number of skin conductance peaks, the difference in experiment segments explains between 32.7% and 44.9% of the variance in physiological reactivity based on the conditional R
2, i.e., based on both fixed and random effects [
34]. There was no significant effect of manipulation on physiological reactivity.
Results of various linear models with reactance as dependent variable and physiological reactivity and/or initial motivational state as predictor variables for the main analysis are presented in
Table 5. Out of the four possible models (
Section 2.5) only three models had a significant fit; the null, state, and full model. The null model includes no predictors and the significant intercept reveals that on average the participants experienced reactance. Results from the state model reveal that from all initial motivational state factors, e.g., attitude, injunctive and subjective norms, only intention to limit meat consumption explains variance in reactance; reported reactance drops 0.54 on a 1–14 point scale with each unit rise of initial intention. We did not find a significant fit for the message-reactivity model, suggesting a minor role of the physiological reactivity variables in explaining variance in reactance. However, the full model was significant, not only including a relationship between reactance and initial motivational state, but also with physiological reactivity. Results of the full model reveal that on average people report 8.73 experienced reactance on a 1–14 scale. Higher initial intentions to limit meat consumption lower the reported reactance by 0.58 per step on the intention scale. Although the physiological reactivity variables were non-significant on their own, they did yield a model with higher predictive power when combined with intention than the state model. Physiological reactivity to the persuasive messages also lowers reactance by 0.23 and 0.52 for each bpm rise in HR and millisecond rise in RMSSD, respectively. In comparison to the null and state models, the full model has the best fit based upon the lowest AIC. The full model explains around 20.1% of variance in self-reported reactance in our sample based on R
2.
4. Discussion
Use of psychophysiological reactions could be an objective approach to personalize persuasive interventions, e.g., trying to avoid reactance. Therefore, we studied psychophysiological reactance to persuasive messages that used high controlling (HCL) or low controlling language (LCL). The messages tried to persuade people with a high meat consumption, e.g., >5 days per week, towards a more vegetarian diet. Psychological reactance to these messages was assessed with self-reported feelings of anger and perceived threat to freedom (PTTF). A factual video preceding the messages ensured that all participants had akin topic-specific knowledge. Motivations to limit meat consumption were measured one week before and immediately after the experiment. Physiological reactivity was measured during the factual video, the persuasive messages, and the closing survey, using features of the cardiovascular and electrodermal system.
We found that neither the HCL nor LCL messages persuaded participants to (further) limit their meat consumption, as indicated by equal attitude and intention levels before and after the experiment. While for the HCL condition this finding was in line with our expectations, we expected the LCL messages to increase motivations to limit meat consumption. As both groups report some level of reactance (at least 2.7 PTTF on a 7-point scale), this might have limited the persuasiveness of the messages. Importantly, participants were more reactant in the HCL condition by experiencing higher feelings of anger and greater perceived threat to freedom compared to participants that received the LCL condition. Therefore, we can assume that, despite the lack of attitude change, our manipulation was successful.
During the persuasive messages, participants had heightened sympathetic physiological arousal as indicated by cardiovascular arousal compared to activity during rest state, i.e., the short baseline. On the other hand, during the factual video, we found decreased heart rate and increased heart rate variability. Because the action performed by the participant was similar during the factual video and the persuasive messages, namely watching an informative video, this finding cannot be attributed to a difference in general information processing or attention. In addition, both the factual video and the persuasive messages were concerned with the context of meat consumption. Generally heart rate decelerates with increased attention [
16], whereas during our persuasive messages the opposite occurs. This finding, therefore, seems to suggest that elevated cardiovascular reactivity is indeed caused by the content of the persuasive messages. We did not find a different effect of the HCL and LCL framing on cardiovascular or electrodermal reactivity. One reason for the lack of this finding could be that the manipulation conditions were not distinct enough in their psychological effects; both conditions were not persuasive and both evoked some level of reactance.
Further analyses reveal a relationship between psychological reactance and initial motivations to limit meat consumption; people with higher intention to limit their meat consumption experienced lower reactance. This finding is not surprising. As the intentions of these people were in line with the advocated appeal, the messages were probably less threatening to them and, thus, evoked lower levels of reactance. Interestingly, adding cardiovascular measures significantly improved this explanatory model. Both an increase in HR and SDNN reactivity appear to lower the reported reactance in this study. These findings are somewhat surprising as increased HR indicates arousal, whereas increased SDNN indicates relaxation. As this is contradictory, future research is needed to replicate this cardiovascular relation. Despite this ambiguity, the combination of initial intention with cardiovascular measures did explain almost twice as much variance in reactance than initial intentions alone, i.e., 20.1% versus 11.2% as indicated by the R
2 in
Table 5. This underlines our idea that psychological reactance might have a psychophysiological nature. It surely invites the combination of subjective self-report with objective physiological measures in future reactance research.
This study has an explorative nature and, thereby, comes with limitations. Since the study was limited to the context of meat consumption and concerns only people that have a high meat consumption patterns (>5 days per week), the findings cannot be generalized. Eating animals is seen as a moral dilemma between the aversion to animal suffering and the desire to eat meat [
25]. The moralization of vegetarianism is driven by strong affective responses, such as disgust and guilt [
35]. Additionally, the formation of these beliefs depend on other attributes, i.e., experiences, characteristics, objects, than health behaviors. [
36]. Thus, the psychology of morality is wired differently than health beliefs. Therefore, it could be that similarly framed persuasive messages concerning other contexts produce different or no physiological markers. Further work is required to establish if this relation also holds in other contexts, e.g., climate change, energy saving.
Another limitation might come from the manipulation not being strong enough, explaining the similar effects of both conditions. Although the spread in reactance scores enabled correlational analyses, it could be that too little people experienced high enough levels of reactance to evoke physiological reactivity. Previous research [
7] reported anger and PTTF scores between 0.45–1.44 and 2.31–3.11 on a 0–4 scale, while we found an average anger score of 3.45 and PTTF score of 4.20 on a 1–7 scale in the HCL condition. The scores are relatively high, but not extreme. This could be one reason for not finding a stronger psychophysiological relationship in reactance. Future research should find out whether higher levels of reactance do reflect in physiology or whether such a robust relationship does not exist at all.
Lastly, personality traits were not considered in this study, while they might have explained some of our results. As mentioned in the introduction, reactant responses are determined by the perceived importance of the freedoms that are threatened [
9]. These perceptions may differ based on personality traits. These traits can therefore mediate the reactance response, but they can also influence the physiological response. For example, trait characteristics such as approach-avoidance motivation are associated with other nervous system activity patterns [
37], novelty seeking correlates negatively with low frequency HRV and LF/HF ratio [
38] and cardiovascular arousal relates to neuroticism and agreeableness [
39]. As our main finding indicates that HRV parameters explain variability in self-reported reactance, personality traits should also be considered in future research.
Despite its limitations, the study adds to our understanding of persuasive messages and their effects on physiology. Future research should try to replicate and extend these findings to different contexts, types of communication, and people. If an evident physiological marker for psychological reactance is found, it could have considerable implications for personalized persuasive technologies, i.e., indicating which messages are (not) effective for the user. It could set up the use of built-in affective loops regulated by physiological, affective, and behavioral interactions in human–technology interaction. Thereby, it would enable physiology-based tailoring as a personalization technique for persuasive technology.