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Review

Appraisal of Functions and Role of Selenium in Heavy Metal Stress Adaptation in Plants

1
Plant Physiology and Biochemistry Laboratory, Department of Botany, Baba Ghulam Shah Badshah University, Rajouri 185234, India
2
Plant Physiology and Biochemistry Laboratory, Department of Botany, Aligarh Muslim University, Aligarh 202002, India
*
Authors to whom correspondence should be addressed.
Agriculture 2023, 13(5), 1083; https://doi.org/10.3390/agriculture13051083
Submission received: 19 April 2023 / Revised: 12 May 2023 / Accepted: 17 May 2023 / Published: 18 May 2023

Abstract

:
Heavy metals (HMs) contamination is one of the main abiotic factors affecting crop productivity and also threatens human health via consuming metal-contaminated crops as a food source. Over the past few years, HMs have drawn a lot of attention due to their increased use for commercial purposes and their harmful effects on plants and other life forms, thus threatening human survival. However, several methods have been adopted in recent years to combat the harsh effects of HMs. After phytohormones, the use of mineral nutrients such as selenium (Se) in the prevention of HM stress has been explored by researchers more recently. Selenium is an important micronutrient widely known for its antioxidant properties in plants and animals. Exogenous Se inhibits metal uptake and translocation and improves the antioxidant system, thus imparting resistance to HM toxicity in plants. Moreover, Se also regulates the production of various osmolytes in cells, which helps develop cell osmolarity. Selenium also produces different secondary metabolites in plants’ defense mechanisms against different stresses. The uptake of mineral nutrients is a vital process for plant growth and development, which is also positively correlated with Se under metalloid toxicity. However, to understand the exact mechanism of Se in HM tolerance, different metabolic processes stimulated by Se and their pathways need to be explored. Hence, this review focuses on the role of Se on nutritional status, antioxidant metabolism, interaction with phytohormones and its role in the regulation of various genes involved in Se-induced HM tolerance. Thus, this study will help researchers in the future for the improvement of HM tolerance via Se application in plants.

1. Introduction

Heavy metal (HM) pollution is one of the significant obstructions in determining the productivity of crops at the worldwide level [1,2,3]. The issue of HM stress has become a prominent concern in various soils and aquatic ecosystems worldwide [4]. Heavy metals are inorganic chemicals non-biodegradable and persistent, causing mutagenic, cytotoxic, and genotoxic effects on plants, humans, and animals. They contaminate soil and water ecosystems and enter food chains [5]. HMs are typically described as metals or metalloids with a higher density (5 g cm−3). For a plant to flourish, certain heavy metals (Fe, Mn, Zn, Cu, Mo, and Ni) must be present in the ideal concentrations as critical micronutrients. While cadmium (Cd), lead, and mercury (Hg) are non-essential and have harmful effects when present in amounts that are beyond the tolerance limit of plants [6]. Exposure to HMs induces plant oxidative stress due to forming free radicals, which alter plant morpho-physiological and biochemical mechanisms at cellular and tissue levels. As a result of their stunted growth and development, plants raised in soils contaminated with HM produce less crops [7]. Heavy metals enter the plant through the root from soil deposits and significantly negatively impact several essential plant functions, including mineral nutrition, photosynthesis, water uptake and ion distribution [8,9,10,11]. Reports suggest that a higher concentration of HMs in the soil increases its uptake and accumulation in several commercial crops such as rice [12,13,14]. However, the increasing concentrations of HMs in agricultural land have negatively impacted the productivity of crops [14,15]. Recently, it has been reported that toxicity reduced photosynthetic functions and nitrogen assimilation, affecting the overall growth of Oryza sativa [11,14]. Besides mineral elements, plants also require a limited number of HMs for their normal functioning and metabolic activities. To prevent crops from metal toxicity, several essential elements have been made, and, among them, Se is widely used because of its various beneficial roles and potential to trigger HM tolerance in plants. Mineral nutrition is one of the key processes that is directly involved in the growth and development of plants. Plants under stressful conditions produce different types of signaling molecules to overcome stress [16]. Selenium is a chief element for plants. Selenium is a broadly distributed trace element with toxic and beneficial effects on plants, humans, and animals. Se might be useful for plant growth and development at low concentrations under optimum and unfavorable conditions [17]. However, the agglomeration of Se on the surface of water or soil may become a source of toxicity for plants. For this reason, most research on plant Se resistance has emphasized Se interaction with sulfur (S) metabolisms [18]. The addition of Se limits the uptake of metals by roots and their translocation to shoots by synthesizing various growth regulators, one of plants’ indispensable stress tolerance mechanisms [19]. The low concentration (<1000 mg Se/kg DW) of Se can reduce metals’ toxic effects on plants and develop tolerance against stress by inducing different metabolic processes [20]. However, at higher concentration (>1000 mg Se/kg DW), Se affects plant growth and decrease the level of organogenesis, nucleic acid synthesis and protein synthesis. The uptake of Se from the soil is controlled by edaphic and plant factors. Primarily, soil is the most common factor controlling Se entry into the plant body [21]. Selenium application reduces the adverse effects of both biotic and abiotic stresses by adjusting different mechanisms like secondary metabolites and antioxidant systems [17]. Selenium application enhanced photosynthesis by activating antioxidant enzymes involved in ROS detoxification under Cd stress in Capsicum annuum L. [22]. Studies have also shown that selenium decreased the levels of ROS and induced lignification of cell walls under cadmium stress in Oryza sativa [23]. However, the detoxification action of Se on the growth of garlic plants under Cd stress was also investigated [24]. Osmolytes, also important components of plant defense systems, are synthesized under stress conditions. Further, the application of Se also induced the production of some amino acids, such as proline (Pro), glycine betaine (GB), etc., under metal stress [25,26]. Nanoparticle treatment of Se also regulates the accumulation of Pro, GB, and sugars in response to HMs stress [27,28]. These amino acids act as osmolytes that help develop tolerance to stress conditions by maintaining their osmotic balance. Moreover, plants also secrete important compounds that participate in plant secondary defense against biotic and abiotic stress conditions. These secretions are commonly known as secondary metabolites (SMs). Among all the classes of SMs, phenolics are one of the major classes of SMs that participate in plant secondary defense. However, the exogenous supply of Se significantly enhanced the levels of phenolic metabolites under different types of metal stresses [17,29,30,31]. Furthermore, antagonistic results of Se on the production of glucosinolates (GLS) were also observed in plants when exposed to metalloid toxicity [32,33,34]. Accumulation of HMs in soil badly affects the uptake of mineral elements from the soil, thereby affecting plants’ overall growth and metabolism. However, the exogenous treatment of Se enhances the uptake of the elements by interfering with metal uptake and hence improves the nutritional status of plants [35,36,37]. Furthermore, Se application also accumulates large amounts of Se in grains under metal stress, thus helping in developing nutritional-rich crop variety [38]. Plant hormones collaborate with the changing environment and are necessary for stress tolerance and crop production [22]. Selenium can occur in soil in four forms: Se, Selenate, Selenite, and inorganic selenide [39]. Under HM stress conditions, the endogenous production of various signalling molecules like ABA, ETH, SA, brassinosteroid (BRs) and JA increased, while auxin (AUX), gibberellins (GA) and cytokinin (CK) levels decreased [40,41,42]. However, the interaction between ETH and ROS was effective in Solanum lycopersicum under HM stress [42]. Ethylene is among the crucial plant stress hormones under adverse environmental conditions. Reports also suggest that Se (10 µM, 5 µM) induced production of ETH is controlled by the type of Se form supplied [43,44]. SeNPs are typically much smaller and have a more uniform size distribution and large surface area than conventional Se preparations. However, the increased levels of ABA and GA in response to selenium nanoparticles (SeNPs) treatment were also observed in Brassica napus [45]. In Cd-stressed Nicotiana tabacum, exogenous Se (10 µM) treatment increased the levels of indole acetic acid (IAA) [46]. However, seed priming with GA and SA showed stimulatory effects on the growth and metabolism of mung bean under Cd stress conditions [47]. Furthermore, the role of JA and GA in inducing tolerance to Cd stress is also explored in Cicer arietinum [48]. However, the interactive role of ETH on Se mediated antioxidant system under Cd stress is studied in Solanum tuberosum [49]. Phytohormones (exogenous/endogenous) alone play a major role in HM tolerance at the molecular level by regulating different defense mechanism genes that contribute to developing tolerance against HM toxicity in plants [50]. However, the information regarding the interaction of Se and phytohormones under HM stress is very scanty. Thus, the present review summarizes the mechanism of Se-induced tolerance to metal stress via regulating different processes such as Se uptake and translocation, antioxidant metabolism and mineral nutrition. This review also highlights the role of Se towards secondary metabolites production and its interaction with phytohormones under metal stress.
The data consolidated in the Figure 1 and Figure 2 were extracted from the subsequent Dimensions software 2.6.0 (https://www.dimensions.ai/). The data were exported on 10 May 2023 using search terms” selenium”, “heavy metal stress,” “stress tolerance,” and “plant” in the dataset. The data retrieved were further filtered by the year-wise distribution of articles published between 2010–2022 and country-wise publications in the past eight years, illustrated in Figure 1 and Figure 2, respectively.

2. Methodology

The review article covers the literature available from 1982 to 2023. The research papers were collected from scientific journals, books, and reports through electronic search PubMed, Google Scholar, and Science Direct, Scopus using keywords. Documentation of the information from these sources helped in drafting the different sections of the manuscript.

3. Selenium Uptake and Translocation

Selenium is a metalloid occurring naturally in sedimentary rocks in dry areas of the world [51]. The inorganic forms of Se i.e., Selenite (SeO32−) and Selenate (SeO42−), are the two main forms of Se which remain available to plants through soil. Selenite and selenate are present in anoxic and oxic soils [52]. Selenium chemically resembles sulfur(S), hence taken up inside the plants via S transporters located in the root plasma membrane [53]. Many species of plants have been identified as Se hyperaccumulators which can accumulate high concentrations of Se, such as Stanleya pinnata [54]. Selenium uptake differs from species to species, soil type, soil concentrations, and form of Se supplied [55]. Selenate absorption by plants is a well-established mechanism. The high-affinity sulphate transporters help in the absorption of selenate by roots. However, the selectivity of various plant species for sulphate and selenate varies [56,57]. On the other hand, nothing is understood about the process behind selenite uptake by plants. It has been observed that plant roots absorb selenite through passive diffusion [58]. Several species of plants belonging to the families Asteraceae, Brassicaceae and Fabaceae absorb higher concentrations of Se in hair-like epidermal appendages called trichomes [59]. The metabolism of Se in higher plants is closely associated with that of S due to their chemical analogy. Both ions SeO32− and SeO42− are absorbed by the plants via roots, and SeO42− is taken via sulphate transporters (SULTRs). This relationship is demonstrated by the fact that Se is taken up by the plants via sulphate transporters and SeO32− via aquaporins and phosphate transporters [52,57,60]. However, considerable differences in the uptake and transport mechanisms of inorganic forms are found in plants [61]. Selenate is uptake by plant roots in an active manner and leads to increased Se concentrations in the roots. However, selenite is not normally concentrated in roots [61]. Selenium is metabolized by S assimilation enzymes resulting in the synthesis of organic forms of Se, i.e., selenocysteine (SeCys) and selenomethionine (SeMT). However, Se uptake can also be increased from the soil via aquaporins by maintaining the pH of apoplastic space through H+-ATPases [62]. Moreover, in rice, it was observed that selenite and SeNPs were accumulated in roots, whereas selenate is more stored in shoots [63]. Selenium uptake can also be enhanced by regulating the expression of SLUTRs [64]. Thus, from the above paragraph, it can be concluded that plants uptake different forms of Se from the soil using different transporters, such as sulfur and phosphate transporters. However, plants also promote the uptake of some forms of Se in an energy-independent manner via aquaporins. Moreover, the organs for different forms of Se accumulation and its transport also vary based on Se accumulated.

4. Selenium and Heavy Metals

Selenium is an essential nutrient for plants which is a foe to some living organisms like bacteria, humans, and most of the chlorophyte species [65,66,67]. Even Se is crucial for oxidative stress resistance, immunity enhancement, and antipathy of heavy metal toxicity [68,69]. Metalloid toxicity is now emerging as a significant concern to both humans and the environment [11,14]. Globally anthropogenic activities are the main cause of metal toxicity in agricultural lands. Heavy metals are non-biodegradable, accumulate in higher trophic levels of the food chain (biomagnification) and threaten biotic forms and their environment [70,71]. In Lactuca sativa, adding selenite significantly reduced the agglomeration of Pb and Cd and, at the same time, increased the uptake of some essential elements, including Se [72]. Selenium promotes the growth of plants and may act as a heavy metal opponent as it is a necessary micronutrient with some anti-oxidative and physiological properties [73,74]. The cadmium existing in water, soil, and the atmosphere can cause serious problems to all organisms and its bioaccumulation in the food chain can be highly hazardous [75]. The presence of Se may affect the distribution of elements which are necessary for plant growth and metabolism. The Se functions to reduce metal toxicity and regulate micronutrient levels by modifying soil conditions like pH, adsorption, and organic matter. It is vital in enhancing metal chelation and improving ROS scavenging mechanism [74,76,77,78]. The beneficial impact of Se on plants is often described as its ability to increase the anti-oxidative capacity of their cells. Se enhances the cellular anti-oxidative mechanism by elevating the enzymatic activity of CAT, GR, SOD and APX, which protects the plant cellular membranes against oxidation [79,80]. An appropriate concentration of Se can delay senescence, improve photosynthesis, and antioxidant capacity, induces auxin levels and promote plant growth [46,81,82]. Selenium application at optimum concentration improved the growth and biomass of Cucumis sativus plants under heavy metals stress (Cd, Pb, Ni) by detoxifying ROS-induced damages via regulation of activities of various antioxidant enzymes [83]. Application of 2 μM Se improved root growth and decreased oxidative stress in Al-treated Lolium perenne [84]. Exogenous treatment of Se reduced As-induced toxicity in Phaseolus aureus by interfering with its uptake and improved plant growth by activating the antioxidant system under As toxicity [85]. However, the application of Se in Pb-stressed Vicia faba plants reduced the accumulation of ROS via enhancement in the activities of various antioxidant enzymes [86]. The application of Se reduced the oxidative stress caused by the combined treatment of Cd and Pb in Brassica napus by minimizing the uptake and accumulation of both Cd and Pb [87]. Selenium plays a pivotal role in neutralizing abiotic stresses in plants. However, the role of Se in response to antioxidant metabolism has been studied under HM stress (Table 1) and found to induce tolerance to different HMs discussed below:
Selenium application is involved in the recovery of chloroplast injuries caused due to Cd stress in Cucumus sativus by increasing starch content in leaves [95]. Exogenous Se (4 µM) alleviated Zn stress by improving growth and photosynthesis in Billbergia zebrina [96]. SeMT (3 µM) mitigated Cd toxicity in mustard species by enhancing enzymatic antioxidants and preventing Cd aggregation in cell organelles [89]. Further, leaf spraying of Se (10, 20, and 40 μM) reduced Cd toxicity by limiting Cd uptake by roots and improved photosynthetic attributes, osmoprotectants and antioxidant levels in Satureja hortensis [90]. However, Se application significantly increased the accumulation of organic Se in grains but also affected the uptake and translocation of other HMs in Triticum aestivum [97]. More interestingly, exogenous supplementation of Se is also involved in promoting flowering in Cucumis sativus, which was delayed by Cd and maintained the sex ratio in plants [98]. Foliar spraying of Se in wheat leaves inhibited metal uptake and enhanced the nutritional status of wheat by accumulating more Se in the grains [38].
Moreover, the application of SeNPs ameliorated the toxic effects of Cd by enhancement in the production of some essential SMs and osmoprotectants in Coriandrum sativum [26]. Supplementation of Se considerably reduced Cd stress in Raphanus sativus by improving photosynthetic attributes, antioxidants level and micro-essential nutrients [35]. In plants, Se has a dual function. At low concentrations, it acts as an antioxidant, but at enormous doses, it generates ROS and impairs several cellular activities. Se affects plant growth, physiology, and antioxidant machinery at various concentration levels [99]. Foliage application of SeNPs alleviated both Cd and Pb toxicity in Salvia officinalis and decreased lipid peroxidation of membranes [100]. Both foliar and root applications of Se enhance plant growth, development, and tolerance. But the foliar application is considered more efficient than the root application [101]. Selenium treatment reduced ROS accumulation in the reproductive parts of rice and increased pollen viability and germination under Cd stress [102]. Researchers revealed that using elements with antioxidant properties can abate the hazardous effects of heavy metal stresses [103]. Figure 3 shows the mechanism of HM-induced changes in the growth and metabolism of plants and the role of Se in alleviating HM-induced oxidative stress by triggering various defense processes in plants. (ROS: reactive oxygen species, PCD: programmed cell death).
Some researchers reported that Se application reduced Cd accumulation and decreased toxicity in pepper by enhancing chlorophyll contents and overall antioxidant activity [104]. In rice seedlings, Se increases the absorption of Cd in the cell wall and vacuoles and decreases the amount of Cd in its toxic form [93]. Optimal doses of Se were known to cope with various biotic and abiotic stresses, including exposure to HMs, by regulating the antioxidant system [105]. Selenium is also a fundamental part of some antioxidant enzymes, which shield cells from ROS damage [29,106]. Additionally, reports also showed that an ideal concentration of Se (1/2 µM) enhanced the antioxidant capabilities of photosynthetic pigments and slowed the ageing of leaf tissues in Brassica oleracea and strawberries [29,107]. Furthermore, adding Se reduced oxidative stress by stimulating the ROS scavenging system and synchronized Cd accumulation in shoots and roots in Brassica juncea [88]. Recent reports also confirm that all the Se (SeNPs, inorganic and organic) forms contribute to HM tolerance by inducing the activities of various antioxidants and other biochemical parameters in Oryza sativa under Cd stress [93].
Selenium plays a significant role in the mitigation of metal-induced toxicity by the improvement in the overall growth and metabolism of plants. Primarily, Se reduces metal uptake and transport by changing root architecture, stimulating different types of antioxidants and PCs, and producing many other types of defensive compounds in plants to increase the tolerance potential of plants against metal toxicity.

5. Selenium Interaction with Osmolytes under Heavy Metal Stress

To avoid cellular damage due to the accumulation of ROS, plants deposit some compatible solutes known as “osmolytes” to protect the cellular machinery from several environmental stresses. Among all the osmolytes, Pro, GB, polyamines, and sugars are the common osmolytes that plants accumulate under stressful conditions and play an essential role in osmoregulation. However, plants synthesize different types of osmolytes under different environmental constraints, and their exogenous supply may also enhance the production of other osmolytes, such as proline application relieved As-induced oxidative damages by improving other osmolytes concentration in mung bean crops [108]. Increment in the levels of Pro and GB with the application of Se (10 µM) demonstrates its role in providing membrane stability in Solanum lycopersicum under Cd stress [25]. Increased production of osmolytes, including Pro, GB, and sugars, protect the cells from damage against stress conditions by developing osmotic potential and regulating other defense mechanisms [88,109]. It has also been reported that Se supplementation to Cd-challenged tomato plants promoted the production of compatible solutes and stress markers, which relieves Cd toxicity [26]. However, applying SeNPs increased Pro accumulation in Coriandrum sativum under Cd stress [26]. However, the leaf spraying of Se elevated leaves Pro content and grain Se concentration in wheat cultivars [110]. Application of SeNPs increased the content of Pro and GB in Glycine max subjected to As stress [27]. Selenium at optimal concentration positively regulated the synthesis of essential osmoprotectants such as Pro and sugars [111]. An increased level of important compatible solute Pro was also observed in Triticum aestivum in response to Se under Cd stress [28]. Osmolytes are essential in maintaining osmoregulation in plants under various stress conditions. These osmolytes, also called osmoprotectants, are synthesized in plants and help develop tolerance to plants against different stresses. Pro, GB, and sugars are the most synthesized osmolytes in plants under stress conditions. However, it has also been observed that Se regulates the biosynthesis of these osmolytes under stress conditions. These osmolytes protect the plants against HM toxicity by maintaining osmolarity and membrane stability and strengthening the antioxidant defence system to minimize ROS-induced damages.

6. Selenium Interplay with Phytohormones under Heavy Metal Stress

Phytohormones have gained much attention over the last two decades due to their wide use in plants against many abiotic stresses. However, their role in ameliorating different strains attracted researchers to more and more use of these signaling molecules. In recent years, much work has been carried out to elaborate on the role of phytohormones against HM stress. These signaling molecules protect plants against HMs by regulating different physiological and biochemical processes that help develop tolerance strategies against stresses [112]. Moreover, their role, along with Se, in alleviating HM stress is the least studied so far. Exogenous application of AUX affected the uptake of Cd and reduced Cd stress by improving photosynthesis and antioxidant system and raising the level of endogenous nitric oxide (NO) in tomatoes [6]. Furthermore, the role of GA application in mitigating Nickel (Ni) toxicity was also observed in mung beans by improving growth and biomass and reducing Ni uptake [113]. However, Se interferes with HMs by regulating the endogenous production of phytohormones [19]. Reports also suggest that inorganic forms of Se negatively affect the production of ethylene [43]. However, their organic forms, such as SeMT, positively regulated ethylene biosynthesis but also affected AUX transport in rice [44]. Moreover, it has been also noticed that optimal concentration of Se induces tolerance to Cd and low phosphorous stress in Nicotiana tabacum by regulating AUX biosynthesis and transport genes [46,114]. However, pretreatment of mung bean seeds with GA and SA improved overall growth and antioxidant system and prevented Cd-induced alterations in roots under Cd stress conditions [47]. Furthermore, applying JA and GA mitigated Cd-induced toxicity in Cicer arietinum by minimizing ROS accumulation via increased antioxidants and osmolytes production under Cd stress [48]. Besides organic and inorganic forms of Se, it can also be applied exogenously as nanoparticles (NPs). However, the literature available on Se so far indicates the effectiveness of Se in the removal of various abiotic stresses. Reports further enlightened that supplementation of SeNPs and ZnONPs (Zinc oxide) in combination improved germination rate and seedling growth by inducing the endogenous levels of ABA and GA in Brassica napus [45]. However, applying these signaling molecules (Phytohormones) impart tolerance to various abiotic stresses through interaction with other signaling molecules and promoting signal transduction cascades in plants [115]. Biochemical and molecular studies have revealed that defense response facilitated by phytohormones such as ETH and JA play an essential role in Se resistance and accumulation [116]. Reports suggest that selenite has been shown to decrease NO levels in a concentration-dependent manner in Arabidopsis root [117]. It has also been reported that the application of NO and SeNPs regulated the expression of the DREB1A gene in Cichorium intybus [118]. However, the endogenous production of ABA under Cd stress also plays a role in the regulation of various Cd-responsive genes such as HsfA4c, HMA4 in roots and HMA2, HMA3CAD and NAS, suggesting its role in the accumulation of more Cd in apoplast region [119].
Studies also emphasized the role of Se and AUX on morphological and biochemical attributes and genotoxicity under As stress in rice plants [120]. It has been reported that the presence of PGRs in the external growth medium of plants has altered ion homeostasis (K+, Na+, Ca2+) in the root tissues and mesocotyle [21]. Reports also indicated that JA and ETH are essential for Se resistance in Arabidopsis plants [18]. Exogenous GR24, a source of strigolactone, also reduced Cd toxicity by inducing NO production and elevated the levels of different antioxidants in Hordeum vulgare [121]. The application of IAA also increased the phytoextraction of Cd/U from the soil in Helianthus annuus [122]. The roots are the foremost organ of plants that reflect heavy metal stress. Thus, the knowledge about the interaction between heavy metal stress and AUX homeostasis is of utmost significance. The involvement of phytohormones in the amelioration of HM stress and their response to different HMs has been studied (Table 2) and discussed in detail below:
In recent times, the participation of plant growth regulators (PGRs) in the establishment and activation of plant defense mechanisms to HMs exposure has been largely studied [132,133,134]. Accumulating evidence indicates that exogenous ABA can stimulate the transport of Pb2+ from underground to aerial parts of herbaceous plants [124]. Exogenous ABA addition enhanced the activities of enzymatic antioxidants that relieve oxidative stress by scavenging ROS produced due to Pb toxicity in Atractylodes macrocephala [135]. SA interacts with other plant hormones, such as AUX, ABA, and GA and encourages the production of antioxidant chemicals and enzymes in response to stress, particularly HM stress [136]. The combined application of Se and phytohormones represents a promising strategy to ameliorate HM toxicity by reducing the uptake of HMs, activating various defense systems in plants as illustrated in Figure 4.
Phytohormones play a crucial role in the growth and developmental processes of plants when exposed to a variety of abiotic stresses. Some of these signaling molecules are produced endogenously during stress conditions. However, their exogenous application to different abiotic stresses, including HM stress, significantly contributes to developing tolerance. Primarily, PGRs interfere with metal uptake via altering root architecture, thus resisting the uptake and accumulation of metals in the plant body. Moreover, Se supplementation also induces the endogenous production of different growth regulators under different stresses. Thus, both Se and PGRs help develop tolerance to stress conditions by regulating different plant mechanisms to minimize oxidative damage.

7. Molecular and Proteomic Interaction of Selenium under Heavy Metals

Work on Se indicated that Se plays a beneficial role in enhancing abiotic stress tolerance [17]. Proteomics is acknowledged as an effective method for understanding how plants respond to abiotic stress, comprising toxic metals such as Cu, As, Al, Cr etc. [137,138]. Analysis of more than a hundred (110) proteins to understand the proteomic response of rice seedlings under different levels of Cr stress was carried out by [139]. In a recent study, RNA sequencing, proteome profiling, and a morphological demonstration were conducted to explore molecular cross-talk of Se mediated As stress amelioration [92]. There are numerous transporters family that play crucial roles in response to As stress in plants, specifically for uptake of As in root, phloem movements, xylem loading and unloading in cells and subcellular organelles specific distribution [92]. The Germin protein family participates in numerous metabolic and stress tolerance mechanisms. Selenium helps plants to cope with As stress by regulating various stress-responsive genes [92]. When exposed to Cd, rice developed Cd-responsive miRNA. The translational levels of several targeted genes were inversely linked with the corresponding miRNA, suggesting the molecular role of HM stress resistance in plants [140]. At the molecular level, Se also plays an essential role in developing HMs tolerance in plants by regulating numerous stress-related genes. However, the involvement of ETH and Se in the regulation of genes which encode enzymes of antioxidants has been observed in mustard subjected to As stress [123]. Significant enhancement in the regulation of WRKY1, PAL and 4CL genes was observed in Mimordica charantia when exposed to slightly higher concentrations of nano-Se [141]. Increased expression of antioxidant genes, PAL and CHS was also noticed in Cr stressed mustard [142]. Selenium treatment significantly up-regulated the expression of S transporters (SLUTs) genes involved in the transport of Se [143]. Application of Se at 25 µM in broccoli has shown negative effects at both translational and transcriptional levels. Proteins of the BIN family, which express during stress conditions and heat shock proteins (HSPs), were badly affected in Se-treated plants.
Moreover, down-regulation of different transcription genes such as MYB (28, 34), BCAT4, MAM1, CYP79B2 and FMO2 was also detected in Se-treated plants [32]. Regulation of various antioxidant and thiol metabolism genes in response to Se was also observed in Oryza sativa exposed to As stress [94]. Exogenous Se considerably increased the expression levels of the NtPT2 gene in tobacco [114]. Selenium also up-regulated genes responsible for seed germination and seedling growth in rapeseed [45]. In Glycine max, SeNPs treatment significantly upregulated various stress marker genes in response to As stress [27]. However, the addition of Se in rice cell culture in the presence of Cd positively up-regulated OsHMA3 and OsPAL, OsCoMT and Os4CL3 genes responsible for Cd accumulation in the vacuole and lignin biosynthesis. On the other hand, Se also represses the expression of genes (OsNramp5, OsLCT1) involved in Cd uptake and transport [23]. Moreover, the expression of SBP1 protein in arabidopsis seedlings indicated its role in alleviating Cd-induced oxidative stress by promoting its binding to Cd [144]. Furthermore, foliar treatment of Se (10 µM) induced genes responsible for Se accumulation and transport to shoots and differentially expressed the proteins related to Se uptake, aggregation, antioxidants, and heat shock proteins [145]. Meanwhile, Se treatment upregulated genes responsible for cell wall lignification and triggered resistance to Cd by inducing the expression of BZR1, LOX3, and NCDE1 genes [22]. Selenium (5, 10 µM) supplementation negatively affected the expression of genes (TaNramp5, TaHMA2) responsible for Cd uptake and translocation but also up-regulated TaHMA3-gene required for Cd translocation to the vacuole in both roots and shoot in wheat [91].
An important area of the plants to respond or increase tolerance potential to stresses, including HM, is the regulation of genes at both transcriptional and translational levels. During stress conditions, activation/expression of different stress-responsive proteins or genes occurs, which help the plant to generate tolerance for survival against stress. In response to metalloid toxicity, Selenium application down-regulates various genes responsible for the uptake and transport of HM in plants, thus indicating its importance in plant stress tolerance. Moreover, Se also regulates the expression of various antioxidant genes that are significant players in developing the tolerance and hence protection of plants against abiotic stress.

8. Selenium Interplay with Secondary Metabolites (Phenolics, N-Containing Metabolites and Terpenes)

In the era of climate change, abiotic stress hampered the growth and development of plants. Plants develop several defense mechanisms to cope with such conditions. Plant secondary metabolites display their functions against stresses [146,147]. The amounts of SMs under stress conditions are slightly higher and are involved in signaling, thereby regulating the activity of genes responsible for plant defense [148]. Among different SMs, terpenes, phenolics and nitrogen compounds are widely known for their protective effects against various environmental constraints [149]. To investigate the role of these compounds in plant defense, the exogenous use of monoterpenoid on Quercus suber leaves indicated its role in endogenous terpene accumulation and improved photosynthesis of plants subjected to heat stress [150]. The production of plant metabolites is regulated genetically, environmental factors and also the mineral status of the plant [151]. Plants accumulate different types of metabolites under varying conditions. For instance, the production of flavonoids during drought stress in Arabidopsis thaliana is thought to be involved in drought tolerance [152]. Matricaria chamomilla plants subjected to copper (Cu) and Cd stress have shown more phenolic content than the non-stressed plants [153].
Similarly, increased levels of phenolics were observed in Vaccinium corymbosum plants facing Cd stress [154]. Increased biosynthesis of phenolics was also observed in Kandelia obovata under HM stress [155]. Increased accumulation of different types of SMs was also noticed in Brassica juncea, which has a higher Cd tolerance capability without external mitigators [156]. To overcome plant metal toxicity, the use of elicitors can be done to enhance SMs production [157]. The effect of Se on SMs production and their function in plants has been studied (Table 3) and discussed in detail below:
Research on selenium has shown that it plays a crucial part in plant metal tolerance by limiting the uptake of metals from the soil by changing the root architecture system and preventing their translocation to leaves [19]. Selenium at low concentrations benefits plant secondary metabolites, as it enhances the levels of carotenoids and phenolic compounds in rice [163]. Selenium nanoparticles supplementation in bitter melon induced the activity of phenylalanine ammonia-lyase (PAL), an important enzyme involved in the biosynthesis of phenolic compounds [141]. The addition of Se upregulated PAL and Chalcone synthase genes and increased the contents of phenolics, flavonoids and anthocyanin compounds in mustard under chromium (Cr) stress [17,31]. The report showed that Se decreased the accumulation of glucosinolates (GLS) in broccoli [33]. In Se-treated Arabidopsis thaliana, a reduction in GLS and carotenoid content was observed in shoots by suppressing their biosynthetic genes [34]. Selenium application negatively affected the accretion of GLS by suppressing genes involved in GLS biosynthesis and their precursors in broccoli [32]. An increment in the levels of plant essential oils (secondary metabolites) in response to Se was also observed in Melissa officinalis [20]. However, the supplementation of Se at low doses increased the accumulation of GLS in Brassica oleracea [29]. In Allium sativum, treatment with Se improved PAL activity and increased the content of phenolics under salt stress [161]. Selenium application increased the levels of total phenolics and flavonoids in Zea mays and Valerianella locusta [159,162]. Recently, it has been reported that spraying of SeNPs on the leaves of Mentha suaveolens induced the accumulation of various types of essential oils under salt stress [158]. Reports also indicate that Se ameliorated the Cd toxicity of Brassica juncea by improving its antioxidant system and SMs status [88]. Selenium also enhances the biosynthesis of flavonoids and tocopherols, which are also involved in relieving oxidative stress of different abiotic stresses [80]. Selenium also attenuated As toxicity in Oryza sativa by inducing the aggregation of phenolics in both underground and aerial parts of the plant [160]. Secondary metabolites are an important class of plant defense compounds synthesized by plants as a response to different types of stress conditions that can cause harm to plants. Plant exudates such as tannins, resins, gums etc., are the common secretions mostly related to stress factors, i.e., both biotic and abiotic. However, the root exudates also limit the uptake and translocation of HMs, thus helping protect plants against HMs toxicity. Based on the results of the aforesaid studies on Se and secondary metabolites, it has been concluded that Se is involved in improving the antioxidant system of plants by enhancing the synthesis of different types of SMs, which play an essential part in the plant defense system. As per the literature, the effect of SMs on forming other SMs can vary depending on the specific metabolites involved, the plant species and the environmental conditions.
In some cases, the synthesis of one SM may stimulate or inhibit the synthesis of another metabolite. Conversely, it is also possible that the synthesis of one SM may not affect the production of another. Overall, the interactions between different SMs in plants are still not fully understood, and more research is needed to determine the specific effects of each compound on the synthesis of others.

9. Selenium Interaction with Mineral Nutrients under Heavy Metal Stress

Selenium is an essential plant micro-nutrient vital in various metabolic pathways in plant life. However, its accumulation in plants varies from species to species. Moreover, at high concentrations, Se shows toxic effects on plants, but at optimal concentrations, it is reported to be safe for plants. However, some plant species can accumulate higher concentrations of Se in their body. Selenium is known for its both beneficial and toxic effects on plants. Apart from developing resistance to metal/metalloid uptake, it is also known to increase nutrient absorption and accumulation in plants. Reports indicated that the coordinative supply of Se and S in mung bean increased the uptake of various mineral nutrients such as NPK, Fe, Zn and S under drought stress [36]. Selenium helps plants relieve oxidative stress by acting as an antioxidant that also improves their nutritional status by enhancing the uptake and aggregation of mineral nutrients [164]. A significant increment in the uptake of some nutrients such as Fe, Mn and Cu was also observed in radishes subjected to Cd stress in the presence of Se [35]. However, the supplementation of Se in the nutrient solution favoured more uptakes of P and Ca from the media, indicating its role in maintaining mineral nutrition in Zea mays [165]. Application of Se significantly enhanced the uptake of micro-nutrients that regulated various metabolic processes to develop tolerance against As stress [160]. Moreover, Se treatment improves the nutritional quality of crops by accumulating more Se in edible parts of plants [38]. Supplementation of Se at different concentrations showed fluctuations in the contents of both macro and microelements in different species [166].
Furthermore, Se at higher concentrations affected the uptake of micronutrients in Eruca sativa [167]. However, improvement in N and Ca content was also observed in wheat subjected to exogenous Se treatment [168]. Selenium supplementation significantly up-regulated various essential mineral elements uptake in Brassica campestris by modifying root architecture to reduce Cr uptake [169]. Introducing the biosynthetic form of SeNPs to different HMs (Cd, Pb, Hg), stress improved the nutritional status of Brassica chinensis by enhancing the uptake of nutrient elements [37].
Availability of mineral nutrients is one of the chief processes in the life cycle of a plant to regulate all its growth and developmental processes. However, the uptake of these mineral elements is severely affected by the soils contaminated with metals, thus affecting the overall growth and metabolism of plants. On the other hand, Se is directly involved in the uptake of these nutrients from the soil by inhibiting metal uptake and therefore helps the plants to restore the activities of all their physiological, biochemical, and developmental processes to withstand such harsh environmental constraints.

10. Conclusions

Rising concentrations of HMs in the soil and their alarming negative effects on plants and, thus, on other living life forms attracted scientists in the field of research more towards this environmental constraint in the present era of science. Due to its increasing threat to the agronomy field, various methods have been adopted by scientists to overcome this problem. Exogenous supplementation of Se in the form of selenite, selenate, SeCys, SeMT and SeNPs has been successfully done to cope with the harsh effects of HM stress in plants. Selenium is directly involved in alleviating HM toxicity by inhibiting or limiting its uptake and translocation from soil to aerial parts of plants. Primarily, Se is involved in improving plants’ growth and photosynthesis by improving nutritional status, osmoprotectants concentration, antioxidant metabolism and secondary metabolites under metal stress conditions.
Further, the crosstalk between HMs, Se and phytohormones is needed to be understood. By understanding this problem, it will become more feasible to overcome such threats, and crops with higher nutritional quality and yield can be produced in the future. And hence, this approach can also become helpful in reducing global food scarcity/hunger. Therefore, future research in this area is likely to shed new light on the molecular, physiological, and ecological mechanisms underlying Se-mediated stress tolerance and to identify new opportunities for the development of sustainable and effective strategies for crop improvement.

Author Contributions

Conceptualization, M.A. (Mohd Asgher) and N.A.K. Validation, A.R., S.N.u.I. and M.A. (Mohd Arshad). Writing—Original Draft, M.A. (Mohd Asgher), A.R., S.N.u.I. and M.A. (Mohd Arshad). Writing and editing, M.A. (Mohd Asgher), N.A.K., A.R., S.N.u.I. and M.A. (Mohd Arshad) Supervision M.A. (Mohd Asgher) and N.A.K.; funding acquisition, M.A. (Mohd Asgher). All authors read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. The number of publications in the literature on the effect of Se, HMs and their coordinated role in plants from 2010–2022.
Figure 1. The number of publications in the literature on the effect of Se, HMs and their coordinated role in plants from 2010–2022.
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Figure 2. The number of publications country-wise that have studied the effect of selenium and HMs on plants in the last eight years.
Figure 2. The number of publications country-wise that have studied the effect of selenium and HMs on plants in the last eight years.
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Figure 3. Role of selenium in the mitigation of heavy metals stress.
Figure 3. Role of selenium in the mitigation of heavy metals stress.
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Figure 4. Supplementation of Se and phytohormones ameliorated heavy metal toxicity via reduced uptake of HMs and activation of various defence systems.
Figure 4. Supplementation of Se and phytohormones ameliorated heavy metal toxicity via reduced uptake of HMs and activation of various defence systems.
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Table 1. Role of Selenium under HM stress conditions.
Table 1. Role of Selenium under HM stress conditions.
Species Heavy Metal Conc. Se Conc.Effect of Se on Antioxidants MetabolismReference(s)
Brassica napus5 µM Cd
500 µM Pb
15 µMEnhanced SOD and GSH-Px levels to minimize Cd/Pb-induced oxidative stress[87]
Brassica juncea300 µM Cr4 µM Strengthen the inheretent defense system by inducing the expression of SOD, CAT, APX, GPOX, GR, GST, DHAR M-DHAR, AsA and GSH against Cr stress.[31]
Brassica juncea100 or 200 µM Cd50 µMIncrement in CAT, APX and GR activity was observed [88]
Brassica spp.50 µM
Cd
3 µMInduced ROS detoxification, maintained the levels of SOD, CAT and POD[89]
Glycine maxAs
25 µM
25 μMReduced As toxicity by improving photosynthesis, antioxidants, and regulation of some defense genes[27]
Vicia faba50 µM Pb6 µMUp-regulated CAT, GPOX and GSH-Px levels[86]
Phaseolus aureus10 µM As5 µMReduced As-induced oxidative stress by increasing CAT, APX GR, AsA and GSH levels[85]
Satureja hortensis150 µM
Cd
40 µMElevated the levels of CAT and POD and reduced Cd toxicity.[90]
Cucumus sativus25 µM Cd
200 µM Ni
100 µM Pb
8 µMStimulated antioxidant system by enhancing the activity of CAT, APX and GPOX[83]
Triticum aestivumCd
50 µM
5 and 10 μMDown-regulation of genes of Cd uptake and transport[91]
Oryza sativaAs
100 µM
25 μMUp-regulated various As-tolerant genes and also induced the antioxidant expression [92]
Oryza sativa20 µM
Cd
1 µMUp-regulated CAT and GSH-Px activity and reduced lipid oxidation[93]
Oryza sativa25 µM
As
25 µMPositively enhanced the activities of CAT, APX, GSH-Px, GR, GST and GSH[94]
Lolium perenne0.2 µM
Al
5 µMReduced lipid peroxidation by Increasing SOD and APX activity [84]
Table 2. Role of phytohormones under heavy metals stress.
Table 2. Role of phytohormones under heavy metals stress.
SpeciesHM Conc.Phytohormones Conc.ResponseReference(s)
Brassica juncea24 µM
As
200 μL/L
Ethephon
Improvement of photosynthetic attributes by reduced As and ABA accumulation, increment in antioxidant activity[123]
Sedum alfredii100 µM Cd0.2 mg/L
ABA
Exogenous ABA enhanced endogenous ABA production, which is involved in Cd tolerance by regulating the expression of Cd tolerance genes[119]
Populus × Canescens3 µM
Pb
10 μM
ABA
Ameliorated the toxic effects of Pb by minimizing oxidative stress and induced expression of genes responsible for Pb resistance[124]
Oryza sativa150 μM
As
3 μM
IAA
Improved growth by accumulating more amino acids, proteins etc.[120]
Cajanus cajan5 µM
Cu2+
1 nM
JA
Improved photosynthesis, antioxidative system and reduced oxidative stress[125]
Solanum lycopersicum3 µM Cd10 nM
HBL
Improved overall growth and productivity of plants, positively regulated N-metabolism.[126]
Zea mays50 µM
Cd
10−9 M
IBA
Reduced Cd toxicity by inducing ROS detoxification and improved nutrient status of plants[127]
Vigna radiata60 µM
Ni
10−4 M
GA3
Improved growth and biomass of plants by reducing the uptake of Ni from soil[113]
Helianthus annuus4 µM
U/Cd
500 mg/L
IAA
ROS detoxification by inducing antioxidants, increased uptake of U/Cd from soil[122]
Brassica juncea50 μM
Cd
200 μL/L
Ethephon
Improved growth, induced antioxidants, and amino acids accumulation[128]
Brassica juncea1.2 µM
Cr
200 μL/L
Ethephon
Mitigated Cr stress by improving photosynthesis, reduced oxidative stress, and also enhanced proline accumulation[15]
Hordeum vulgare10 μM
Cd
1 μM
GR24 (strigol analogue)
Reduced Cd toxicity by improving photosynthesis, uptake of essential nutrients, and inducing stress markers[121]
Panicum virgatum10 µM
Cd
1 μM
GR24 (strigol analogue)
Increment in photosynthetic parameters, stimulation of antioxidant system and improvement in the mineral status of plants[129]
Solanum lycopersicum150 μM Cd100 μM
NO
Positive effect on photosynthesis and improved growth, antioxidant system, osmoprotectants and secondary metabolites accumulation, hence involved in Cd tolerance.[130]
Oryza sativa5 µM
Cr
0.1 nM
24-EBL
Induced Cd detoxification by enhancing photosynthesis and regulating the genes expression[131]
Table 3. Effect of Se on the production of secondary metabolites.
Table 3. Effect of Se on the production of secondary metabolites.
Plant SpeciesSe DoseSecondary MetabolitesResponseReference(s)
Mentha suaveolens10 µMProduction of essential oils such as piperitenone oxide, limonene, jasmone etc.Enhanced growth and SMs production[158]
Brassica juncea4 µMEnhanced levels of phenols, flavonoids, and anthocyaninsImprovement in the photosynthesis thereby growth of plant by inducing the accumulation of antioxidants, SMs, etc., against Cr stress[31]
Brassica oleracea25 µMAffected the production or accumulation of glucoraphanin, an important glucosinolateReduced content of glucosinlates precursors, Suppressed the expression of genes involved in glucosinlates biosynthesis[32]
Melissa officinalis5 µMIncreased the accretion of essential oils such as z-citral, citral and geranyl acetatePositive effect on the growth at low concentration[20]
Brassica oleracea10 µMIncrement in the levels of phenolic compounds and glucosinolatesImproved growth and yield characteristics of plants by inducing antioxidant and SMs levels[30]
Zea mays10 µMEnhancement of phenols and flavonoids contentInduced accumulation of proteins, sugars and SMs[159]
Oryza sativa25 µMInduced the accumulation of essential phenolics such as gallic, protocatechuic and ferulic (acids)Increased the uptake of nutrients from the soil and regulated SMs production, hence reducing As toxicity[160]
Brassica juncea50 µMEnhanced phenolic contentReduced Cd stress by inducing the antioxidant system and SMs status of plants[88]
Allium sativum4 µMIncreased total phenolic contentIncreased tolerance ability of garlic to salt stress by preventing membrane oxidation, phenols accumulation and regulation of phenylalanine ammonia-lyase activity[161]
Vallerianella locusta5 µMEnhanced the endogenous levels of flavonoids and phenolicsImproved growth, antioxidant activity and accumulation of SMs[162]
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Asgher, M.; Rehaman, A.; Islam, S.N.u.; Arshad, M.; Khan, N.A. Appraisal of Functions and Role of Selenium in Heavy Metal Stress Adaptation in Plants. Agriculture 2023, 13, 1083. https://doi.org/10.3390/agriculture13051083

AMA Style

Asgher M, Rehaman A, Islam SNu, Arshad M, Khan NA. Appraisal of Functions and Role of Selenium in Heavy Metal Stress Adaptation in Plants. Agriculture. 2023; 13(5):1083. https://doi.org/10.3390/agriculture13051083

Chicago/Turabian Style

Asgher, Mohd, Abdul Rehaman, Syed Nazar ul Islam, Mohd Arshad, and Nafees A. Khan. 2023. "Appraisal of Functions and Role of Selenium in Heavy Metal Stress Adaptation in Plants" Agriculture 13, no. 5: 1083. https://doi.org/10.3390/agriculture13051083

APA Style

Asgher, M., Rehaman, A., Islam, S. N. u., Arshad, M., & Khan, N. A. (2023). Appraisal of Functions and Role of Selenium in Heavy Metal Stress Adaptation in Plants. Agriculture, 13(5), 1083. https://doi.org/10.3390/agriculture13051083

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