Abscisic-Acid-Modulated Stomatal Conductance Governs High-Temperature Stress Tolerance in Rice Accessions

Round 1

Reviewer 1 Report

Comments for author File: Comments.pdf

Author Response

Please see the attachment

Author Response File: Author Response.doc

Reviewer 2 Report

Manuscript Title: Abscisic acid modulated stomatal conductance governs high temperature stress tolerance in rice accessions

Journal: Agriculture

Authors: M. K. Malini¹, Sourabh Karwa¹, Payal Priyadarshini¹, Pramod Kumar¹, Shivani Nagar¹, Mahesh Kumar¹, Sudhir Kumar¹, Viswanathan Chinnusamy¹, Renu Pandey^1* and

Madan Pal^1*

Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi, India-110012

 

Reviewer Recommendation: Major Revision

 

Reviewer’s comments to Authors

1.      Grammatical errors are present, please revise the whole manuscript to remove any possible grammatical and typos errors.

2.      Error in sentence formation, please revise the whole manuscript to avoid the use of long sentences.

3.      In the keywords, it is strongly advisable to use suitable words that can aid in finding out the manuscript in current registers or indexes. Strictly avoid the use of title words in the keywords.

4.      Please maintain uniformity while in-text citation and referencing.

5.      The reference does not meet the format requirements of the Journal so please check the references as per the author’s guideline of the Journal.

6.      There are some of the citations that did not mention in the text and reference list, so a critical check and corrections of the references appeared in the text and list are required.

For example, below reference cited in the reference list but missing in the text.

Manh HD, Tuyet OT. 2020. Larvicidal and Repellent Activity of Menthaarvensis L. Essential Oil against Aedesaegypti. Insects. 11:198. doi:10.3390/insects11030198.

 

Abstract and Introduction:

1. The abstract was not clear and the objective of the paper was not clearly validated from the abstract.
2. Add significant results from all the sections precisely in the abstract.
3. Novelty statement is omitted from the manuscript which is important for emphasizing the exclusivity of your study.
4. Line 13, page 1, the spelling mistake was there i.e., stge, it should be stage. Please revise it.
5. Line 19, page 1, please provide the abbreviation full form before using in the manuscript, For example: HT
6. The future perspective of the experiment should be mentioned in the abstract.
7. The literature from past work done in the same field missing to strength the introduction section.
8. Add the objectives of your study at the end of ‘Introduction’ section.
9. “Though it is a light-dependent processbut is highly sensitiveto high temperature” there must be some space between ‘process but’ and ‘sensitive to’ as they seem one word.
10. “In many studies, heat-tolerant rice varietiesare characterized by higher photosynthetic rates, increased membrane thermostability and heat avoidance”, ‘heat avoidance’ word does not seem correct in this place, please rephrase.

 

Materials and Methods:

1.     “Pots were kept flooded (water 3–5 cm above the soil surface) until two weeks before the physiological maturity”, what duration was considered for physiological maturity, please mention.

2.      “For each accession, three plants were selected per treatment and single plant basis measurements were done for traits number of tillers and panicles, panicle weight, grain weight per panicle and shoot biomass”, single plant basis measurements can be considered significant for confirming any final conclusion?

3.      “the flow rate was maintained at 500 μmol s^-1, CO₂ adjusted to 400 μmol mol^-1 using CO2 mixer”, maintain uniformity as “CO2” is written in two different ways in a single line, it reflects that the manuscript is vaguely written.

4.      “Photosynthetic Water Use Efficiency (pWUE) and Carboxylation Efficiency (CE) were calculated as the ratio of Photosynthetic rate to stomatal conductance (Pn/gs)”, both were calculated in same way? Then what is the difference in the two parameters?

  Results and Discussion:

1.      “On the other hand in accession IRGC127222 the increased due to high temperature was marginally compare to other accession”, rephrase this sentence as it is incorrect.

2.      “Under HT condition, significant decrease in flag leaf Fv/Fm was observed and the rate of reduction was highest in sensitive accession IRGC127222 (21%), followed by 14 per cent reduction in CX99 and 9 per cent in NL 44”, maintain uniformity, either use ‘%’ sign or use ‘percent’ across the manuscript, and this decrease is in comparison to which treatment, please specify.

3.      Discussion section must include more studies from previous reports as evidence for your study, recent studies must be included. Follow and cite some of the recommended important papers in the reference section of this comment.

4.      The authors failed to explain all of their findings in the discussion section with specific mechanisms. It is advisable to maintain balance between the result and the discussion section.

References:

There are lots of statements throughout the manuscript that essentially require proper validations and citations with previous studies which are seriously missing in the present manuscript. Introduction, Result, and Discussion sections poorly cited with the references and strongly recommended to update and validation with previous studies. Therefore, the relevant papers listed below should be considered and cited appropriately in the Introduction, Result, and Discussion sections of this manuscript which will certainly upgrade and enhance the Ms. quality significantly. Omitting any of these citations will certainly compromise the scientific quality of this manuscript.

·     Agnihotri, A., et al. (2020). Does jasmonic acid regulate photosynthesis, clastogenecity, and phytochelatins in Brassica juncea L. in response to Pb-subcellular distribution? Chemosphere, 243, 125361.

·       Kumar, D., et al. (2022). Photosynthesis, lipid peroxidation, and antioxidative responses of Helianthus annuus L. against chromium (VI) accumulation. International Journal of Phytoremediation, 24(6), 590-599.

·         Yadav, M., et al. (2022). Foliar application of α-lipoic acid attenuates cadmium toxicity on photosynthetic pigments and nitrogen metabolism in Solanumlycopersicum L. ActaPhysiologiaePlantarum, 44(11), 1-10.

Conclusion:

1.      The conclusion section should be in accordance to the result and discussion section, random addition of results should be avoided.

2.      Conclusion section failed to enlighten the spirit of the finding and is missing the results. Revise it precisely.

3.      Conclusion section must also include the future perspectives of this study which is lacking in the manuscript.

 

Tables and Figures:

1.      The legends of the figures are not crisp and not completely bringing out the sense of the figures. Rewrite it accordingly.

2.      The placement of tables and figures in the manuscript should be done appropriately, which is missing in this manuscript. Please revise it.

Author Response

Please see the attachment.

Author Response File: Author Response.doc

Round 2

Reviewer 2 Report

Dear Editor

I asked the authors to address the following issue but the authors did not consider this. As a reviewer I strongly feel that this will certainly compromise the Ms quality. Editor should ensure that the reviewer's concern should be addressed. Without Addressing this issue I can not recommend the paper for publication.   Thanks and regards  

There are lots of statements throughout the manuscript that essentially require proper validations and citations with previous studies which are seriously missing in the present manuscript. Introduction, Result, and Discussion sections poorly cited with the references and strongly recommended to update and validation with previous studies. Therefore, the relevant papers listed below should be considered and cited appropriately in the Introduction, Result, and Discussion sections of this manuscript which will certainly upgrade and enhance the Ms. quality significantly. Omitting any of these citations will certainly compromise the scientific quality of this manuscript.

·     Agnihotri, A., et al. (2020). Does jasmonic acid regulate photosynthesis, clastogenecity, and phytochelatins in Brassica juncea L. in response to Pb-subcellular distribution? Chemosphere, 243, 125361.

·       Kumar, D., et al. (2022). Photosynthesis, lipid peroxidation, and antioxidative responses of Helianthus annuus L. against chromium (VI) accumulation. International Journal of Phytoremediation, 24(6), 590-599.

·         Yadav, M., et al. (2022). Foliar application of α-lipoic acid attenuates cadmium toxicity on photosynthetic pigments and nitrogen metabolism in Solanumlycopersicum L. ActaPhysiologiaePlantarum, 44(11), 1-10.