1. Introduction
With more than 10 million cultivated hectares, sugarcane (
Saccharum officinarum L.) is one of the main agricultural activities that drive the Brazilian economy, making Brazil the largest sugar producer and the second largest ethanol producer worldwide [
1]. Among the producing states, São Paulo stands out with 55% of the total production in the country [
1]. To reach this level, major investments have been made in technologies used in establishing and managing the crop, including strategies for integrated pest management. Even so, countless insects attack and damage sugarcane, and among the most important are the soil dwelling pests that feed on the root system.
The sugarcane billbug,
Sphenophorus levis (Vaurie) (Coleoptera: Curculionidae), has assumed great importance in recent years, mainly because it occurs in areas of São Paulo state and surrounding states [
2]. The main damage to the crop is caused by the insect’s immature phase, when the larvae open tunnels in the plant’s rhizome, which can result in the death of 50 to 60% of tillers, losses in production of up to 30% and reduction in plant longevity [
3]. This insect is attracted to and infests mainly the areas fertigated with vinasse, which is the liquid fraction generated from the alcohol production [
4] and discarded onto the sugarcane fields.
To minimize losses, chemical insecticides have been used extensively in sugarcane pest management. However, the chemical insecticides tend to have low efficiency against
S. levis because the larvae feed inside the roots and the adults remain buried or below the straw; therefore, the targets are hard to reach [
3,
5]. Thus, alternative strategies must be developed such as the use of entomopathogenic nematodes (EPNs) of the genera
Heterorhabditis and
Steinernema (Nematoda: Heterorhabditidae, Steinernematidae). These nematodes are effective in controlling various species of curculionid pests, including those in the genus
Sphenophorus in the USA and Japan [
6,
7]. Both genera of nematodes exclusively attack insects and have similar life cycles, starting with an infective juvenile (IJ) that carries an entomopathogenic bacterium inside its intestine and releases the symbiont into the insect host’s hemocoel [
8]. The nematodes and symbiotic bacteria act together to overcome the insect’s immune system, causing its death within 24 to 48 h [
9]. Subsequently, the bacteria spread and multiply inside the insect and the nematode reproduces by feeding on both. When food is depleted, the nematode is induced into the IJ form and leaves the insect’s cadaver in search of a new host [
10].
The nematode
Steinernema brazilense Nguyen et al. (isolate IBCB 06) was effective in controlling
S. levis in greenhouse and field tests, causing 80% mortality of larvae within rhizomes and 60% mortality of adults in the field [
2,
11,
12], and with gains in yield of up to 17 tons of cane per ha [
3]. Despite these results,
S. brazilense may not be the most suitable nematode for a biological control program as it is a relatively large species (1157.2 µm) [
13] and therefore generates lower yields in commercial production processes compared to smaller nematodes, such as
S. rarum (Doucet, 1986) Mamiya, 1988 (511 µm) [
14]. In addition, little is known about the effect of vinasse on EPN populations after its application to the field for sugarcane fertigation, which is a very common practice in Brazil.
Thus, the present study aimed to assess the nematode
S. rarum (isolate Pam 25), a native nematode found in South and North America [
15,
16,
17], regarding its ability to search for insects within the cane rhizome, its performance at different application rates, survival in vinasse and field efficacy to control
S. levis as well as the other sugarcane soil-inhabiting pests,
Hyponeuma taltula Schaus (Lepidoptera, Erebidae, Herminiinae) and
Leucothyreus alvarengai (Frey) (Coleoptera, Melolonthidae, Rutelinae).
2. Material and Methods
For all the experiments conducted under laboratory and greenhouse conditions, nematodes were produced in vivo using
Galleria mellonella (L.) (Lepidoptera, Pyralidae) as a host [
18]. For the field experiments, nematodes were produced in vitro by the sponge process [
19].
2.1. Ability to Search for the Host
The nematode
S. rarum (Pam 25), isolated from soil samples of the Pampa biome (31°42′1.5′′ S 54°14′9.8′′ W) located at Aceguá, RS, Brazil, was compared with
S. carpocapsae (Weiser) (IL 1), isolated from a population of larvae and pupae of
S. levis in a cane field (21°54’38” S 46°54’34” W) at São João da Boa Vista, SP, Brazil [
20], and with
H. bacteriophora Poinar (HBEN01), commercially available in Brazil, with respect to the nematodes’ ability to search for larvae of two insect hosts (
G. mellonella and
S. levis) within the cane rhizome.
2.1.1. Galleria Mellonella as an Insect Host
For this study, G. mellonella larvae were used within the cane rhizome to simulate the behavior of S. levis larvae. The use of G. mellonella as an alternative host was due to the great difficulty of rearing S. levis in the laboratory, or to obtain its larvae in sufficient quantities. There were eight treatments: the three nematodes applied to insect larvae inserted into the cane rhizome (partially buried in the soil), the three nematodes applied to the larvae just buried in the soil and the two controls consisting of larvae held inside the rhizome or larvae buried in the soil (without EPNs applied). Each treatment had 4 replications. Each replication was represented by a plastic pot (1.8 L, 14 cm diameter) containing 900 mL of fine sand (10% moisture) plus 4 insect larvae.
For the treatments with the cane rhizome,
G. mellonella larvae were held in small metal cages (3 cm long) and introduced individually into 10 cm long sugarcane rhizomes, through a 5 cm deep and 1.5 cm wide hole drilled at one of the sectioned ends of the rhizome. One larva was inserted per rhizome. After the insertion of the larvae, the holes were filled with cane fibers (simulating the frass left by
S. levis larvae) and the rhizomes were buried 5 cm deep in the sand (10% moisture) with the holes facing the soil (
Figure 1). Four rhizomes were buried per pot. This methodology was devised considering that
S. levis penetrate into the cane rhizome, which allows EPNs to enter the tunnels caused by the insect’s larvae and, consequently, reach the host [
20].
To verify the natural ability of the three EPN species to find the host in the soil, G. mellonella larvae were individually held in the cages and buried superficially. For both situations (larvae inserted in the rhizome and buried in the soil), the nematodes were tested at a rate of 100 IJs/insect (2.6 IJs/cm2), which was applied with the aid of a pipette at a volume of 5 mL/pot. The pots were incubated at 25 °C and with a 12-h light–dark period, and larval mortality was assessed one week after application. Dead larvae were transferred to Petri dishes (9 cm) over a filter paper slightly moisturized with water to confirm the cause of death, if by the nematodes, characterized by nematode reproduction. The experiment was conducted twice, maintaining the same conditions for each trial.
2.1.2. Sphenophorus levis Larvae as a Host
For this study, S. levis larvae collected from the sugarcane field, at 2°/3° instar, were used as the host. The methodology used was the same as mentioned above using G. mellonella, except that the S. levis larvae were held freely inside the rhizome, not inside a cage as used for G. mellonella, in order to allow the insect to feed on the inner tissue of the rhizome.
There were eight treatments: the three nematodes applied to insect larvae inserted into the cane rhizome (partially buried in the soil), the three nematodes applied to the larvae just buried in the soil and the two controls consisting of larvae held inside the rhizome or larvae buried in the soil (without EPNs applied). Each treatment had four replications. Each replication was represented by a plastic pot (1.8 L, 14 cm diameter) containing 900 mL of fine sand (10% moisture) plus four insect larvae
For the larvae buried in the soil, insects were held individually inside cages containing a small piece of stem for feeding. The experiment was conducted twice, maintaining the same conditions for each trial.
2.2. Performance at Different Application Rates
This study assessed the ability of S. rarum (Pam 25) to search for the host inside the rhizome at five different rates. The experiment was conducted under greenhouse conditions with the goal of estimating the best rate to control S. levis populations in sugarcane fields. For this study, as for the previous one, G. mellonella larvae were used as a model host. The experiments were conducted in basins with 40 cm diameters, which represented 40 cm of cane row.
Two experiments were conducted. One with larvae of
G. mellonella held individually in metal cages and inserted into the cane rhizome partially buried in the soil; and another with larvae of the insect held individually in the cages and buried in the soil. For each experiment, there were five treatments represented by the five nematode rates: 125, 500, 2000, 8000 and 32,000 IJs/40 cm of row; therefore, rates were represented as number of IJs per 40 cm of cane row. A non-treated control was also included. Each treatment had four replications, with each replication consisting of a plastic basin (40 cm diameter × 20 cm depth) containing 8 kg of fine sand (10% moisture) plus five insect larvae. Thus, five rhizomes were partially buried per basin (
Figure 2).
The rest of the methodology was the same as used in the previous study, except the insects inside and outside the rhizomes were arranged in a row along the diameter of the basin, 5 cm apart; the nematodes were applied in a volume of 10 mL next to both sides of the row; and, after application, the soil was covered with cane straw (10 cm thick) to simulate conditions of sugarcane fields. The experiment was conducted twice, maintaining the same conditions for each trial, with temperature ranging from 20 to 28 °C.
2.3. Survival in Vinasse
This study aimed to assess the compatibility of vinasse with the nematode S. rarum (Pam 25). There were six treatments: liquid vinasse, cane straw moistened with vinasse, sand moistened with vinasse, cane straw moistened with water, sand moistened with water and just water. Vinasse was obtained at the Company Abengoa, São João da Boa Vista, SP.
Each treatment had three replications, each replication consisting of a cylindrical plastic pot (500 mL) containing the nematode plus the substrate. For straw treatments, 20 g of straw was dipped in 500 mL of water or vinasse until the liquid was absorbed (80 mL). Subsequently, the excess liquid was discarded, and the moisturized straw was transferred into the pots. For sand treatments, 80 g of sand was moistened with water or vinasse until reaching field capacity. The sand with a final moisture content of 17% was then transferred to the pot. For treatments without straw and sand, 50 mL of water or vinasse was added to the pots.
S. rarum was applied to each substrate in a 2 mL suspension containing 10,000 IJs. The pots were capped and incubated in a chamber with controlled temperature (25 °C). Evaluations were carried out after 3, 6, 12, 24 and 48 h, as well as after 4, 8, 15 and 30 days, based on 3 samples of about 0.5 to 1 g, taken at random from each pot. Each sample was transferred to a Petri dish containing 10 mL of water, and the IJs found were evaluated whether alive or dead to determine viability. For straw and sand substrates, the samples were slightly shaken/stirred with the help of a spatula to force the release of the IJs into the water and allow their visualization separately from their substrates. At least 100 IJs were counted randomly per sample.
2.4. Field Test I—Efficacy of Nematodes
This study aimed to assess combinations between S. brazilense (IBCB 06) (isolated from soil samples collected in Porto Murtinho, MT, Brazil, 22°10′16′′ S, 57°07′ W) and S. rarum (Pam 25) for the control of S. levis and H. taltula populations in a sugarcane field. The experiment was carried out in a sugarcane field (variety CTC 20) (21°53′56.53′′ S, 46°57′35.48′′ W) fertigated with vinasse, with high levels of S. levis and H. taltula infestations in the previous ratoon. Fertigation began on 1 September 2017, and nematodes were applied three days after fertigation when the cane plants were 0.3 m high, after the second ratoon.
There were three treatments including S. brazilense alone, S. rarum alone, a mixture of the two nematodes and a non-treated control group. Each treatment and control had six replications with each replication consisting of a 100 m long plot with three rows of cane (spaced 1.5 m apart). The plots were spaced laterally from each other by another 3 rows of cane, thus being 4.5 m apart. Therefore, each plot was 450 m2, comprising a total experimental area of 10,800 m².
The nematodes were applied at 1–2 cm depth in the soil of the cane rows, with the aid of three-row disc cutters, coupled to a tractor, covering the entire plot in each pass of the tractor. The nematodes were applied at a rate of 2 × 10
8 IJs/ha, and for the mixture of the two species, each was used at half the rate. This rate was chosen based on a previous study with
S. brazilense [
3] and considering that rates higher than 3 × 10
8 IJs/ha are cost-prohibitive for sugarcane farmers. Evaluation was carried out 21 days after the application by removing 10 cane clumps per plot, each spaced 10 m apart. To remove clumps, trenches of 0.5 × 0.5 × 0.5 m were made. After the removal of each clump, live specimens of
S. levis and
H. taltula found within the cane rhizomes or in the soil around the roots of the plants (larvae, pupae and adults) were counted.
2.5. Field Test II—Rate Effects
The goal of this study was to assess the effect of two rates of S. rarum on the control of S. levis and L. alvarengai in a sugarcane field. Two identical experiments were carried out in two closed areas of a sugarcane field (variety CTC 20), 400 m apart (21°55′17.24′′ S, 47°1′20.75′′ W), with high levels of S. levis and L. alvarengai infestations in the previous ratoon. The first experiment was set up on 25 September and the second on 26 September 2019, when the cane was about 0.3 m high, after the third ratoon, three months after the last harvest.
For each experiment, there were three treatments which consisted of two rates of S. rarum (1 × 10⁸ IJs/ha and 3 × 10⁸ IJs/ha), and the insecticide fipronil (Regent 800WG) at 1.2 L/ha; a non-treated control was also included. Each treatment and the control, in each experiment, had 4 replications, with each replication consisting of a 100 m long plot with 3 rows of cane (1.5 m between rows). The plots were spaced laterally from each other by another 3 rows of cane; thus, rows were 4.5 m apart from each other. Therefore, each plot was 450 m2, comprising a total experimental area of 14,400 m2.
The nematode and the chemical insecticide were applied at 1–2 cm deep in the soil of the cane rows, with the aid of three-row disc cutters, coupled to a tractor, covering the entire parcel in each pass of the tractor. Evaluations were carried out 21 days after each application by removing six cane clumps per plot, spaced 15 m apart. For the removal of clumps, trenches of 0.5 × 0.5 × 0.5 m were made. After the removal of each clump, the live insects of S. levis (larvae, pupae and adults) found inside the rhizomes of the cane or in the soil around the roots of the plants, and larvae of L. alvarengai found in the soil, were counted.
2.6. Statistical Analyses
The first two experiments with
G. mellonella and
S. levis larvae (effect of nematode species and insect placement) were conducted as a factorial with the effects of nematode species and insect placement as the main factors. If the effects of nematode species and insect placement were found to act independently (no interaction detected between them), then the analysis focused solely on these main effects, and simple effects were not elucidated further [
21].
For the experiment in the greenhouse (testing different rates of S. rarum on G. mellonella larvae buried in the soil as well as those held inside the rhizome), data were analyzed by logarithmic regression between rate and mortality.
For all experiments, except the one in the greenhouse (testing different rates of
S. rarum on
G. mellonella larvae buried in the soil as well as those held inside rhizome), treatment effects were detected using analysis of variance (ANOVA). If the F value was significant, then treatment differences were further elucidated with Tukey’s test (
p < 0.05). Numerical data (insects’ populations) were square root transformed prior to analysis, and percentage data were arcsine transformed (arcsine of the square root) [
22]; nontransformed means are presented in the
Section 3.
All statistical comparisons were conducted using SPSS version 16.0 software, with a p-value of ≤ 0.05 indicating significance.
4. Discussion
The entomopathogenic nematodes S. rarum, S. carpocapsae and H. bacteriophora caused >90% mortality of G. mellonella and S. levis larvae buried outside the rhizome, indicating that they were equally attracted and virulent to the insects. The differential results obtained with G. mellonella larvae inside the rhizomes indicate that S. carpocapsae and H. bacteriophora were probably less able to enter the rhizomes and infect the targets. Moreover, the similar trends of performances for the three nematodes tested on larvae of G. mellonella and of S. levis inside the rhizome validate the use of G. mellonella as an alternative host model to select EPNs for the control of S. levis.
The native EPN species
S. rarum (Pam 25) which originated from soils of the Pampa biome, RS, Brazil, showed the best performance to reach and kill larvae of
G. mellonella within the cane rhizome but did not differ from
S. carpocapsae (IL1) when larvae of
S. levis were used as a target within the rhizome.
Steinernema carpocapsae (IL 1) was found causing natural infections in the population of
S. levis within the cane rhizome [
20] but has not been able to keep the insect population below the threshold in the sugarcane area, which suggests the need for an inundative application with this or another more efficacious nematode. Comparing
S. brazilense with
H. indica against larvae of
S. levis inside the rhizome, the former EPN showed significantly better performance (80% mortality) compared to the latter (35%) at the lowest dose tested (150 IJs/insect or 2.4 IJs/cm
2) [
11].
In the present study, the holes in the rhizome were filled with cane fibers to simulate the frass left by
S. levis in the field. Thus, another aspect that may have contributed to the better performance of
S. rarum is its smaller size (511 µm) when compared to
S. carpocapsae (558 µm) and
H. indica (588 µm) [
23], allowing the nematode to enter narrower openings in the rhizome to reach the insect, and in the host to reach its hemocoel [
24,
25,
26,
27]. Their performances were assessed just one week post-inoculation; thus, additional research is needed to assess the efficacy of three nematode species in different substrates with different organic contents [
28], and over a longer period in laboratory, greenhouse and field conditions. It is not clear at this point whether the superior activity of
S. rarum against
S. levis may have been due solely to higher efficiency in penetrating the host material, or whether
S. rarum may possess higher levels of innate virulence, or a better host-seeking ability to the target pest; additional research is needed to elucidate the basis for the increased virulence observed in our study.
Steinernema rarum exhibits an intermediate behavior between ambush and cruiser when
G. mellonella larvae are the host, with a preferential searching site at the depth of 0–2 cm below the soil surface [
29]. This characteristic is of great importance to the control of
S. levis because most of the holes left by this insect in the cane rhizome are located below the soil surface (90 %), mainly at the depth of 0–2 cm (35.23%) [
20].
Steinernema rarum has been studied for more than three decades, and its potential to control insects of different orders, including curculionids, is well known [
30]. Comparing the virulence of
S. rarum, S. feltiae and
H. bacteriophora on 33 different species of insects distributed in the orders Anoplura, Coleoptera, Diptera, Hemiptera, Hymenoptera, Lepidoptera and Orthoptera, with a rate of 100 IJs/insect [
31],
Steinernema rarum stood out for causing mortalities above 90% for most tested insects, considered of agricultural importance:
Eriopis connexa (Germar)
, Hippodamia convergens (Guerin-Meneville)
, Naupactus cinereidorsum Hustache,
Epicauta adspersa (Klug)
, Tenebrio molitor L. and
Chrysodina sp.
Steinernema rarum also stood out for its successful development in adult and immature insects, while the other two species of nematodes developed only in immature insects.
Sugarcane vinasse is a residue from the sugar–ethanol industry, and it has been mostly used in fertigation practices, i.e., utilizing it as a liquid fertilizer for crops, reducing the water input for plant growth [
32]. However, vinasse was toxic to
S. rarum when IJs were suspended in the compost and kept for more than 6 h, but not when in contact with the straw and soil moistened with the vinasse. Vinasse is already known to affect the life cycle and reproduction of phytoparasitic nematodes. Two species of phytopathogenic nematodes,
Meloidogyne incognita (Kofoid & White) and
Meloidogyne javanica (Treub), were exposed to soils containing different concentrations of vinasse [
33]. After 90 days, there was a drastic decrease in reproductive activity and in the number of eggs for both due to the increased concentrations, especially for
M. incognita, which was almost completely inhibited. The nematicidal effect that resulted from the application of vinasse was due to the increase in organic matter provided by this by-product in the soil, generating compounds that stimulate the reproduction of organisms antagonistic to nematodes, such as fungi, bacteria and other microorganisms. In addition, the decomposition of organic matter promotes the release of organic acids that may negatively affect nematodes [
34].
In the present study, vinasse affected the nematode only in liquid form, probably due to the high concentration of salts (potassium, sulfate, nitrogen, magnesium, calcium and others) and organic matter [
35,
36], not its low pH value (4.2). Nematodes tolerate a wide pH range, from 4 to 8, but not an increase in the concentration of salts [
37]. Organic matter, on the other hand, reduces the availability of oxygen in the liquid vinasse [
38], also affecting the suspended IJs. Oxygen becomes a limiting factor in clay soils, water-saturated soils or soils with a high organic content [
39]. Sugarcane vinasse is reported to be a nitrogen-deficient medium, which is mostly composed of acid-insoluble nitrogen [
40]. Vinasse is also characterized as a feedstock rich in phenolic compounds and melanoidins [
41].
In straw and soil, vinasse did not affect the nematode, probably due to the lower moisture content, dilution of nitrogen and salts and increased oxygenation. Potassium, phosphorus, calcium, nitrate and other salts are adsorbed on solid materials of the soil when the vinasse is added in moderate doses [
35]. Although the liquid vinasse caused high mortality for
S. rarum, viability remained high (> 80%) for up to 6 h, which would allow for EPN application in the field together with vinasse during fertigation (as long as the vinasse is at an adequate temperature).
In the first field test,
S. rarum showed the same efficacy as
S. brazilense to control
S. levis and
H. taltula, with no improvement observed when applying the mixture of both species. In a similar study, mixtures of
S. carpocapsae and
H. bacteriophora were less effective against larvae of
Diabrotica speciosa compared to these species tested alone [
42]. On the other hand, application of two EPN species with different foraging strategies was superior to single-species application when targeting two insect hosts that occupy different soil niches [
43].
In the second field test, the results obtained to control
S. levis coincided with those obtained in greenhouse tests, where five rates of
S. rarum were tested on
G. mellonella larvae inserted within the cane rhizome and buried in the soil. Based on the linear equation (
Figure 6), the dose to kill 50% of
G. mellonella inserted in the rhizome was 3082 JIs/40 cm of row, which represents 5.1 × 10
7 JIs/ha considering that 1 ha has 6666 m of row (1.5 m apart). Thus, by the equations obtained in the greenhouse tests, the doses of 1 × 10
8 IJs/ha and 3 × 10
8 IJs/ha would result in 57% and 70.7% mortality, respectively, levels very close to those obtained in the field, 60% and 71%, respectively. These data highlight the methodologies used in the laboratory and greenhouse tests as valid for the selection of species and doses of EPNs aiming at the control of
S. levis.
Entomopathogenic nematodes have been used in the USA and Japan to control
Sphenophorus spp. in lawns, pastures and maize [
6,
44,
45,
46]. The control levels of these insects with EPNs are usually above 70%, but with a dose (2.5 × 10
9 IJs/ha) well above that tested in the present study (1–3 × 10
8 IJs/ha). In the present study, the satisfactory results (>60% of control) obtained with lower doses/ha (1–3 × 10
8 IJs/ha) were due to the high susceptibility of the
S. levis larvae, the use of the previously selected
S. rarum and its application directed to the rows of sugarcane planting. Considering the cane rows are 1.5 cm apart, the applied nematode reached the concentration of 7.5 IJs/cm
2 in the 20 cm wide strip of each row, where larvae, pupae and recently emerged adults of
S. levis were located. For most insects, EPNs are effective only if high rates are applied (e.g., a minimum of 25 IJs/cm
2) [
6]. For
S. levis, remaining inside the rhizome as larvae and pupa probably led these stages to evolve toward higher susceptibility to EPNs because they remain protected from the entomopathogens in their cryptic habitat and thus do not develop resistance. In contrast,
Sphenophorus species that occur in the USA feed on the root but may stay outside, buried in the soil [
6]. Conceivably, insects tend to be more resistant to EPNs if they are naturally exposed to the soil during their life cycle [
43,
47,
48].
Steinernema rarum caused 56.3–58.4% control of
L. alvarengai, levels which are slightly above those already obtained with
S. brazilense for the control of this insect (50%) [
3]. These results can be considered satisfactory in the biological control of
S. levis,
S. alvarengai and
H. taltula, especially if compared to those obtained with fipronil. It appears that application of
S. rarum for control of
S. levis will provide additional benefits in controlling other sugarcane pests such as
L. alvarengai and
H. taltula, but additional trials to measure efficacy against these two pests are needed. Moreover, the experiments conducted in laboratory and field conditions proved vinasse is compatible with infective juveniles of
S. rarum when applied to the soil or to the straw. However, further studies should also be carried out in order to assess the persistence of
S. rarum in a sugarcane field with and without fertigation using vinasse.
This study is the first to assess
S. rarum in a sugarcane field and highlight its potential to control
S. levis in the crop. Prior studies also indicated that EPNs are able to reach
S. levis within the cane root, and that larvae, pupae and newly emerged adults are quite susceptible to EPNs [
7,
10,
20]. The use of
S. rarum presents itself as a viable alternative to the control of
S. levis and other sugarcane soil insect pests and can be recommended at concentrations of 1–3 × 10
8 IJs/ha, with satisfactory results and without adversely affecting the environment. Application should be conducted immediately after the cane harvest or at the beginning of the plant’s development when the tractor can still enter the field. In Brazil, this period comprises April to December when the populations of larvae and pupae reach the highest peaks [
5]. In drier seasons, soil moisture may be preserved by the thick layer of straw covering the ground or by the frequent application of vinasse, favoring a better performance by EPNs; thus, application of vinasse may be recommended along with the nematode application. Expanded field tests are needed to further optimize application of
S. rarum for control of
S. levis and other sugarcane pests.