Stearoyl-CoA Desaturase Activity and Gene Expression in the Adipose Tissue of Buffalo Bulls Was Unaffected by Diets with Different Fat Content and Fatty Acid Profile

Round 1

Reviewer 1 Report

: In this manuscript, the researchers tried to explain Stearoyl-CoA desaturase activity and gene expression in the adipose tissue of buffalo bulls fed diet with different fat content and fatty acid profile. It is an interesting work .

-     The grammar errors should be checked in the whole manuscript.

-       In abstract, the first four lines should be summarized.

-       In introduction, the main objective has been repeated so it should be refined.

-       Some recent and relevant articles may be added as many of articles have been published on this topic.

-       Conclusion should be refined as it is not properly written as per results.

Author Response

Attached you can find the replies to revision 1.

Author Response File: Author Response.pdf

Reviewer 2 Report

all the abbreviations should be defined on first time. 

Include some latest references and remove the old ones. 

Add sex of the bufflaoes. 

add the name of fatty acids in table 4. 

In the current study, the authors have investigated Stearoyl-CoA desaturase activity and gene expression in the adipose tissue of buffalo bulls fed diets with different fat content and fatty acid profile. The study is important and interesting from several point of view both to the nutritionists and buffaloes meat consumers. The study results are rare in its nature and provides new insight into the research area. Title is well developed although could be better modified to the contents of the study. Introduction provides the updated review of the previous works done in the domain although most recent references could be added to improve the quality of the paper.  Materials and methods are comprehensive and detailed. Results and discussion are concise although complete. conclusion has been well drawn on the basis of the results although it could be shortened.

title of the paper should be modified according to the contents of the paper. 

Line 24-25: remove the readings in parenthesis.

Line 29: add this sentence in the beginning of the abstract. 

.... please give more information  and exact information of the statistical analysis. Why you did not use t-test?
---I think the results of Table 2 should be added in the materials and method section. 
....please give me more possible reasons why there was no significant difference in the fatty concentration of the two diets
Table 4: in the third column, i think it is the p value and not the SEM
A table of forward and reverse RNA sequence should be provided used in the study. 

Author Response

Attached you can find the replies to reviewer 2.

Author Response File: Author Response.pdf

Reviewer 3 Report

The paper is an interesting study of the lack of difference in fatty acid composition of buffalo muscle after feeding a high or low fat diet.

The introduction makes some claims about health benefits from buffalo meat, but these are not supported by the references give. For example, in lines 38-40 claims are made with no supporting reference and the reference in the previous sentence suggest buffalo meat may be healthy, but provides no data bout human health. This report should avoid claims about health aspects unless supported by good studies in humans.

However, the research itself is valid and useful in showing the consistency of muscle fat content, despite dietary differences.

Minor comments

Line 67. “Kilo bites” should be “kilobytes”, although thye abbreviation Kb may not need writing out in full.

Line 70. “polimorphysm” should be “polymorphism”.

Line 73. “GCCA-la” should be “GCCAla.

Line 184. “k” should probably be “j”. Also “Ti” should be “Di” if it matches Di in line 185.

Table 4. It is not clear whether the values shown in columns LF and HF are standard error or standard deviation. In either case, some of the SEM values appear far too high, e.g. C16:0, C18:1 trans-10, C18:1 cis-9, C18:1 cis-11. Some other values appear to be very low, so all results may need to be checked. Despite these anomalies, the conclusion that there is little difference between LF and HF groups seems valid.

Line 264. “found” should be “find”

Author Response

Attached you can find the replies to reviewer 3.

Author Response File: Author Response.pdf