Coordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Germination Assay
2.2. Measurement of Chlorophyll Content
2.3. Quantification of H2O2 Content
2.4. Quantification of Superoxide Content
2.5. Measurement of Nonenzymatic Antioxidants Content
2.6. Measurement of Activities of Antioxidant Enzymes
2.7. RNA Extraction and Expression Analysis
2.8. Statistical Methods
3. Results
3.1. Germination Rate of Different Elite Maize Inbred Accessions under Low Temperature
3.2. Variation in Seedling Establishment among Maize Accessions under Low Temperature
3.3. Ameliorated Oxidative Stress in LT-Tolerant Accession
3.4. Coordinated Antioxidant Defense Systems in LT-Tolerant Accession
3.5. Transcriptional Profile Analysis of AsA-GSH Pathway
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
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Xiao, S.; Xu, T.; Wang, Y.; Xing, J.; Wang, R.; Su, A.; Wang, S.; Song, W.; Zhao, J. Coordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature. Agriculture 2021, 11, 1160. https://doi.org/10.3390/agriculture11111160
Xiao S, Xu T, Wang Y, Xing J, Wang R, Su A, Wang S, Song W, Zhao J. Coordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature. Agriculture. 2021; 11(11):1160. https://doi.org/10.3390/agriculture11111160
Chicago/Turabian StyleXiao, Senlin, Tianjun Xu, Yuandong Wang, Jinfeng Xing, Ronghuan Wang, Aiguo Su, Shuaishuai Wang, Wei Song, and Jiuran Zhao. 2021. "Coordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature" Agriculture 11, no. 11: 1160. https://doi.org/10.3390/agriculture11111160