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Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa Silage

College of Grassland Science and Technology, China Agricultural University, Beijing 100093, China
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Agriculture 2020, 10(1), 10; https://doi.org/10.3390/agriculture10010010
Received: 13 November 2019 / Revised: 23 December 2019 / Accepted: 24 December 2019 / Published: 31 December 2019
In this study, an experiment was performed to evaluate the effect of lactic acid bacteria and 3-phenyllactic acid (PLA) on the fermentation quality and chemical composition of alfalfa silage. Several PLA-tolerant strains were screened from silages and identified. The selected strains (1 × 106 colony forming units/g fresh alfalfa) and PLA (1.0, 2.0, or 3.0 g/kg) were applied to alfalfa before ensiling. After 45 days of storage, the silages were unsealed and subjected to component analysis. Biochemical methods and 16S rDNA gene sequencing were used for the identification of the two strains as Lactobacillus plantarum. The characteristics of chemical and fermentation compounds indicated that PLA and the two strains efficiently improved the quality of the alfalfa silage. It can be concluded that the use of the strains and PLA can significantly improve the quality of silage. View Full-Text
Keywords: 3-phenyllactic acid; alfalfa silage; Lactobacillus plantarum; strain screening 3-phenyllactic acid; alfalfa silage; Lactobacillus plantarum; strain screening
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Wu, Z.; Xu, S.; Yun, Y.; Jia, T.; Yu, Z. Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa Silage. Agriculture 2020, 10, 10.

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