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Open AccessArticle

Insight in miRNome of Long-Term Non-Progressors and Elite Controllers Exposes Potential RNAi Role in Restraining HIV-1 Infection

1
AIDS Immunopathology Unit, National Center for Microbiology, Institute of Health Carlos III, Majadahonda, 28220 Madrid, Spain
2
HIV Unit, Hospital Clínic de Barcelona, 08036 Barcelona, Spain
3
Sección de Enfermedades Infecciosas, Medicina Interna, Complejo Hospitalario de Navarra, 31008 Pamplona, Spain
*
Authors to whom correspondence should be addressed.
J. Clin. Med. 2020, 9(8), 2452; https://doi.org/10.3390/jcm9082452
Received: 19 June 2020 / Revised: 17 July 2020 / Accepted: 29 July 2020 / Published: 31 July 2020
(This article belongs to the Section Molecular Diagnostics)
Long-term non-progressors (LTNP) and elite controllers (EC) represent spontaneous natural models of efficient HIV-1 response in the absence of treatment. The main purposes of this work are to describe the miRNome of HIV-1 infected patients with different extreme phenotypes and identify potentially altered pathways regulated by differentially expressed (DE) miRNAs. The miRNomes from peripheral blood mononuclear cells (PBMCs) of dual phenotype EC-LTNP or LTNP with detectable viremia and HIV-infected patients with typical progression before and after treatment, were obtained through miRNA-Seq and compared among them. The administration of treatment produces 18 DE miRNAs in typical progressors. LTNP condition shows 14 DE miRNA when compared to typical progressors, allowing LTNP phenotype differentiation. A set of four miRNAs: miR-144-3p, miR-18a-5p, miR-451a, and miR-324 is strongly downregulated in LTNP and related to protein regulation as AKT, mTOR, ERK or IKK, involved in immune response pathways. Deregulation of 28 miRNA is observed between EC-LTNP and viremic-LTNP, including previously described anti-HIV miRNAs: miR-29a, associated with LTNP phenotype, and miR-155, targeting different pre-integration complexes such as ADAM10 and TNPO3. A holistic perspective of the changes observed in the miRNome of patients with different phenotypes of HIV-control and non-progression is provided. View Full-Text
Keywords: HIV-1 infection; long-term non-progressors; elite controllers; miRNA-Seq; miRNome; differential expression; biomarker discovery HIV-1 infection; long-term non-progressors; elite controllers; miRNA-Seq; miRNome; differential expression; biomarker discovery
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MDPI and ACS Style

Ayala-Suárez, R.; Díez-Fuertes, F.; Calonge, E.; De La Torre Tarazona, H.E.; Gracia-Ruíz de Alda, M.; Capa, L.; Alcamí, J. Insight in miRNome of Long-Term Non-Progressors and Elite Controllers Exposes Potential RNAi Role in Restraining HIV-1 Infection. J. Clin. Med. 2020, 9, 2452. https://doi.org/10.3390/jcm9082452

AMA Style

Ayala-Suárez R, Díez-Fuertes F, Calonge E, De La Torre Tarazona HE, Gracia-Ruíz de Alda M, Capa L, Alcamí J. Insight in miRNome of Long-Term Non-Progressors and Elite Controllers Exposes Potential RNAi Role in Restraining HIV-1 Infection. Journal of Clinical Medicine. 2020; 9(8):2452. https://doi.org/10.3390/jcm9082452

Chicago/Turabian Style

Ayala-Suárez, Rubén; Díez-Fuertes, Francisco; Calonge, Esther; De La Torre Tarazona, Humberto E.; Gracia-Ruíz de Alda, María; Capa, Laura; Alcamí, José. 2020. "Insight in miRNome of Long-Term Non-Progressors and Elite Controllers Exposes Potential RNAi Role in Restraining HIV-1 Infection" J. Clin. Med. 9, no. 8: 2452. https://doi.org/10.3390/jcm9082452

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