Association of HSV-1 and Reduced Oral Bacteriota Diversity with Chemotherapy-Induced Oral Mucositis in Patients Undergoing Autologous Hematopoietic Stem Cell Transplantation
Abstract
:1. Introduction
2. Experimental Section
2.1. Study Design and Sampling
2.2. Autologous HSCT Procedures
2.3. Evaluation of Candida spp. and HSV in the Oral Cavity
2.4. Analysis of Bacterial Communities
2.5. Statistical Analysis
3. Results
3.1. OM Is Associated with the Detection of HSV-1
3.2. Chemotherapy-Accompanied Dysbiosis of Buccal Mucosal Bacteriota Is Associated with Severe OM
3.3. The Presence of HSV-1 after Chemotherapy Is Associated with the Dysbiosis of Buccal Bacteriota
3.4. The Severity of OM Is Correlated with Depletion of Commensals
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Control (n = 15) | Patients Undergoing Autologous HSCT | p Values | ||||
---|---|---|---|---|---|---|
Total (n = 46) | OM-Free (n = 26) | OM-Ex (n = 20) | p1 | p2 | ||
Age | 38.1±11.6 | 52.7 ± 9.8 | 52.0 ± 10.0 | 53.5 ± 9.8 | < 0.001 a | 0.626 a |
Gender (% male) | 40 | 54.3 | 42.3 | 70 | 0.338 b | 0.062 b |
Type of disease | ||||||
Multiple myeloma (%) | 60.9 | 65.4 | 55 | 0.714 b | ||
Lymphoma (%) | 32.6 | 26.9 | 40 | |||
Others (%) | 6.5 | 7.7 | 5 | |||
HSV-1 (%) | 0 | 23.9 | 19.2 | 30 | 0.038 b | 0.401 b |
Candidaspp. (%) | 37 | 34.6 | 40 | 0.711 b | ||
ANC < 500/μL(%) | 2.2 | 0 | 5 | 0.254 b | ||
ALC < 1000/μL(%) | 69.6 | 65.4 | 75 | 0.487 b | ||
Prophylactic antibiotic use (% yes) | 100 | 100 | 100 | 0.249 b | ||
Prophylactic antifungal use (% yes) | 100 | 100 | 100 | |||
Prophylactic acyclovir use (% yes) | 4.3 | 15.4 | 0 | 0.498 b |
Variables | OM | OM (yes vs. no) | OM Severity (OMAS) | |||||
---|---|---|---|---|---|---|---|---|
No (n = 54) a | Yes (n = 25) a | Odds Ratio | 95% CI | pb | β | pb | ||
HSV-1 | Negative | 63% | 25% | 3.668 | 1.512–8.895 | 0.004 | 0.321 ± 0.223 | 0.151 |
Positive | 37% | 75% | ||||||
Candida spp. | Negative | 64.8% | 66.7% | 1.008 | 0.362–2.807 | 0.988 | 0.064 ± 0.215 | 0.765 |
Positive | 35.2% | 33.3% | ||||||
Bacterial diversity c | 1.89 | 1.95 | 0.604 | 0.275–1.328 | 0.210 | −0.533 ± 0.220 | 0.015 | |
(0.77–3.88) | (0.36–3.68) |
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Lee, A.; Hong, J.; Shin, D.-Y.; Koh, Y.; Yoon, S.-S.; Kim, P.-J.; Kim, H.-G.; Kim, I.; Park, H.-K.; Choi, Y. Association of HSV-1 and Reduced Oral Bacteriota Diversity with Chemotherapy-Induced Oral Mucositis in Patients Undergoing Autologous Hematopoietic Stem Cell Transplantation. J. Clin. Med. 2020, 9, 1090. https://doi.org/10.3390/jcm9041090
Lee A, Hong J, Shin D-Y, Koh Y, Yoon S-S, Kim P-J, Kim H-G, Kim I, Park H-K, Choi Y. Association of HSV-1 and Reduced Oral Bacteriota Diversity with Chemotherapy-Induced Oral Mucositis in Patients Undergoing Autologous Hematopoietic Stem Cell Transplantation. Journal of Clinical Medicine. 2020; 9(4):1090. https://doi.org/10.3390/jcm9041090
Chicago/Turabian StyleLee, Ahreum, Junshik Hong, Dong-Yeop Shin, Youngil Koh, Sung-Soo Yoon, Pil-Jong Kim, Hong-Gee Kim, Inho Kim, Hee-Kyung Park, and Youngnim Choi. 2020. "Association of HSV-1 and Reduced Oral Bacteriota Diversity with Chemotherapy-Induced Oral Mucositis in Patients Undergoing Autologous Hematopoietic Stem Cell Transplantation" Journal of Clinical Medicine 9, no. 4: 1090. https://doi.org/10.3390/jcm9041090