Magnetic-Activated Cell Sorting (MACS): A Useful Sperm-Selection Technique in Cases of High Levels of Sperm DNA Fragmentation
Abstract
1. Introduction
2. Experiment Section
2.1. Study Population and Design
2.2. Sperm DNA Fragmentation (SDF) Assessment
2.3. Male Evaluation and Conventional Sperm Selection
2.4. MACS Sperm-Selection Technique
2.5. Cycle Procedure and Outcome
2.6. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Study Group MACS (n = 366) | Control Group w/o MACS (n = 358) | p | |
---|---|---|---|
Female age (year) a | 372 ± 3.6 | 36.7 ± 3.5 | 0.3 |
Male age (year) a | 40.0 ± 15.7 | 38.7 ± 15.4 | 0.28 |
SDF (%) a | 28.9 ± 8.2 | 29.6 ± 9.1 | 0.30 |
Total sperm count (mil) a | 89.1 ± 79.4 | 95.1 ± 87.2 | 0.15 |
Progressive motility (%) a | 35.2 ± 18.1 | 33.3 ± 16.7 | 0.12 |
Number of collected oocytes | 11.3 ± 7.2 | 11.9 ± 6.8 | 0.36 |
Number of Metaphase II oocytes | 9.7 ± 5.0 | 9.5 ± 5.1 | 0.31 |
Study Group MACS (n = 366) | Control Group w/o MACS (n = 358) | p | |
---|---|---|---|
Fertilization rate (%) | 75.1 | 73.3 | 0.133 |
Pregnancy rate (%) | 60.7 | 51.5 | 0.014 |
Miscarriage rate (%) | 14.7 | 20.6 | 0.034 |
Livebirth rate (%) | 47.4 | 31.2 | 0.001 |
Preimplantation Genetic Testing for Aneuploidy (PGT-A) Cycles | |||
Study Group MACS (n = 126) | Control Group w/o MACS (n = 116) | p | |
SDF (%) | 28.8 | 30.1 | 0.168 |
Fertilization rate (%) | 76 | 76.8 | 0.201 |
Pregnancy rate (%) | 60.4 | 50.6 | 0.121 |
Miscarriage rate (%) | 15.1 | 11.4 | 0.307 |
Live-birth rate (%) | 43.4 | 31.6 | 0.127 |
Autologous Oocyte ICSI Cycles | |||
Study Group MACS (n = 121) | Control Group w/o MACS (n = 120) | p | |
SDF (%) | 30.7 ± 10.2 | 30.8 ± 10.8 | 0.958 |
Fertilization rate (%) | 76.8 | 75.1 | 0.586 |
Pregnancy rate (%) | 52.2 | 50.0 | 0.424 |
Miscarriage rate (%) | 11.3 | 25.5 | 0.005 |
Live-birth rate (%) | 40.9 | 24.6 | 0.03 |
Oocyte-Donation Cycles | |||
Study Group MACS (n = 121) | Control Group w/o MACS (n = 120) | p | |
SDF (%) | 27.7 ± 6.8 | 28.0 ± 7.2 | 0.959 |
Fertilization rate (%) | 76.85 | 76.9 | 0.750 |
Pregnancy rate (%) | 69.6 | 53.9 | 0.013 |
Miscarriage rate (%) | 17.9 | 22.5 | 0.247 |
Live-birth rate (%) | 51.8 | 29.4 | 0.03 |
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Pacheco, A.; Blanco, A.; Bronet, F.; Cruz, M.; García-Fernández, J.; García-Velasco, J.A. Magnetic-Activated Cell Sorting (MACS): A Useful Sperm-Selection Technique in Cases of High Levels of Sperm DNA Fragmentation. J. Clin. Med. 2020, 9, 3976. https://doi.org/10.3390/jcm9123976
Pacheco A, Blanco A, Bronet F, Cruz M, García-Fernández J, García-Velasco JA. Magnetic-Activated Cell Sorting (MACS): A Useful Sperm-Selection Technique in Cases of High Levels of Sperm DNA Fragmentation. Journal of Clinical Medicine. 2020; 9(12):3976. https://doi.org/10.3390/jcm9123976
Chicago/Turabian StylePacheco, Alberto, Arancha Blanco, Fernando Bronet, María Cruz, Jaime García-Fernández, and Juan Antonio García-Velasco. 2020. "Magnetic-Activated Cell Sorting (MACS): A Useful Sperm-Selection Technique in Cases of High Levels of Sperm DNA Fragmentation" Journal of Clinical Medicine 9, no. 12: 3976. https://doi.org/10.3390/jcm9123976
APA StylePacheco, A., Blanco, A., Bronet, F., Cruz, M., García-Fernández, J., & García-Velasco, J. A. (2020). Magnetic-Activated Cell Sorting (MACS): A Useful Sperm-Selection Technique in Cases of High Levels of Sperm DNA Fragmentation. Journal of Clinical Medicine, 9(12), 3976. https://doi.org/10.3390/jcm9123976