Metabolomic Investigation of β-Thalassemia in Chorionic Villi Samples
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Preparation
2.2. Gas Chromatography Mass–Spectrometry Analysis and Data Processing
2.3. Statistical Analysis
2.4. Pathways Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Patients (n) | Age (years) | Gestational Age (weeks) | NT (mm) | CRL (mm) | |
---|---|---|---|---|---|
Control | 27 | 36.8 ± 5.3 | 12.72 ± 0.75 | 2.07 ± 0.9 | 61.34 ± 9.8 |
Heterozygotes | 7 | 31.2 ± 4.1 | 12.2 ± 1.8 | 1.4 ± 0.4 | 53.0 ± 5.4 |
Homozygotes | 6 | 34.7 ± 2.9 | 11.9 ± 0.55 | 1.5 ± 0.3 | 52.9 ± 5.2 |
Supervised Models | ||||||
---|---|---|---|---|---|---|
N | R2X | R2Y | Q2 | p-Value | Permutation Test: Intercept R2\Q2 | |
C vs. Hom vs. Het | 39 | 0.351 | 0.484 | 0.159 | ns | 0.27/−0.14 |
Het vs. Hom | 13 | 0.515 | 0.699 | 0.246 | ns | 0.73/−0.03 |
C vs. Het | 34 | 0.352 | 0.689 | 0.390 | 0.003 | 0.44/−0.21 |
C vs. Hom | 33 | 0.486 | 0.917 | 0.658 | <0.0001 | 0.55/−0.33 |
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Monni, G.; Murgia, F.; Corda, V.; Peddes, C.; Iuculano, A.; Tronci, L.; Balsamo, A.; Atzori, L. Metabolomic Investigation of β-Thalassemia in Chorionic Villi Samples. J. Clin. Med. 2019, 8, 798. https://doi.org/10.3390/jcm8060798
Monni G, Murgia F, Corda V, Peddes C, Iuculano A, Tronci L, Balsamo A, Atzori L. Metabolomic Investigation of β-Thalassemia in Chorionic Villi Samples. Journal of Clinical Medicine. 2019; 8(6):798. https://doi.org/10.3390/jcm8060798
Chicago/Turabian StyleMonni, Giovanni, Federica Murgia, Valentina Corda, Cristina Peddes, Ambra Iuculano, Laura Tronci, Antonella Balsamo, and Luigi Atzori. 2019. "Metabolomic Investigation of β-Thalassemia in Chorionic Villi Samples" Journal of Clinical Medicine 8, no. 6: 798. https://doi.org/10.3390/jcm8060798