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Open AccessArticle

Identification and Expression of Neurotrophin-6 in the Brain of Nothobranchius furzeri: One More Piece in Neurotrophin Research

Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, I-80137 Naples, Italy
Interdepartmental Center for Research in Biomaterials (CRIB) University of Naples Federico II, I-80125 Naples, Italy
Center for Advanced Biomaterials for Healthcare-Istituto Italiano di Tecnologia, I-80125 Naples, Italy
Laboratory of Biology [email protected], Scuola Normale Superiore, I-56126 Pisa, Italy
Laboratory of Biology of Aging, Leibniz Institute on Aging-Fritz Lipmann Institute, D-0445 Jena, Germany
Department of Sciences and Technologies, University of Sannio, I-82100 Benevento, Italy
Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, I-80121 Naples, Italy
Author to whom correspondence should be addressed.
These two authors shared the co-authorship.
J. Clin. Med. 2019, 8(5), 595;
Received: 13 March 2019 / Revised: 19 April 2019 / Accepted: 22 April 2019 / Published: 30 April 2019
(This article belongs to the Special Issue Cutting Edge Preclinical Models in Translational Medicine)
Neurotrophins contribute to the complexity of vertebrate nervous system, being involved in cognition and memory. Abnormalities associated with neurotrophin synthesis may lead to neuropathies, neurodegenerative disorders and age-associated cognitive decline. The genome of teleost fishes contains homologs of some mammalian neurotrophins as well as a gene coding for an additional neurotrophin (NT-6). In this study, we characterized this specific neurotrophin in the short-lived fish Nothobranchius furzeri, a relatively new model for aging studies. Thus, we report herein for the first time the age-related expression of a neurotrophin in a non-mammalian vertebrate. Interestingly, we found comparable expression levels of NT-6 in the brain of both young and old animals. More in detail, we used a locked nucleic acid probe and a riboprobe to investigate the neuroanatomical distribution of NT-6 mRNA revealing a significant expression of the neurotrophin in neurons of the forebrain (olfactory bulbs, dorsal and ventral telencephalon, and several diencephalic nuclei), midbrain (optic tectum, longitudinal tori, and semicircular tori), and hindbrain (valvula and body of cerebellum, reticular formation and octavolateral area of medulla oblongata). By combining in situ hybridization and immunohistochemistry, we showed that NT-6 mRNA is synthesized in mature neurons. These results contribute to better understanding the evolutionary history of neurotrophins in vertebrates, and their role in the adult brain. View Full-Text
Keywords: neurotrophin-6; phylogeny; LNA probe; riboprobe; neuroanatomy; fish; aging neurotrophin-6; phylogeny; LNA probe; riboprobe; neuroanatomy; fish; aging
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Leggieri, A.; Attanasio, C.; Palladino, A.; Cellerino, A.; Lucini, C.; Paolucci, M.; Terzibasi Tozzini, E.; de Girolamo, P.; D’Angelo, L. Identification and Expression of Neurotrophin-6 in the Brain of Nothobranchius furzeri: One More Piece in Neurotrophin Research. J. Clin. Med. 2019, 8, 595.

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