Next Article in Journal
Environment and Male Fertility: Effects of Benzo-α-Pyrene and Resveratrol on Human Sperm Function In Vitro
Previous Article in Journal
Biomarkers in the Diagnosis, Management, and Prognostication of Perioperative Right Ventricular Failure in Cardiac Surgery—Are We There Yet?
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle

UNR/CSDE1 Expression Is Critical to Maintain Invasive Phenotype of Colorectal Cancer through Regulation of c-MYC and Epithelial-to-Mesenchymal Transition

1
Translational Oncology Division, OncoHealth Institute, FIIS-Fundacion Jimenez Diaz University Hospital, Autonomous University of Madrid, 28040 Madrid, Spain
2
Department of Pathology, Clinico San Carlos University Hospital, 28040 Madrid, Spain
*
Authors to whom correspondence should be addressed.
J. Clin. Med. 2019, 8(4), 560; https://doi.org/10.3390/jcm8040560
Received: 27 March 2019 / Revised: 11 April 2019 / Accepted: 23 April 2019 / Published: 25 April 2019
(This article belongs to the Section Molecular Medicine)
  |  
PDF [6620 KB, uploaded 26 April 2019]
  |  

Abstract

CSDE1 (cold shock domain containing E1) gene is located upstream of the N-RAS locus, and codes for an RNA-binding protein named Upstream of N-Ras (UNR). In cancer, CSDE1 has been shown to regulate c-Fos, c-Myc, Pten, Rac1, or Vimentin. UNR/CSDE1 has been studied in breast, melanoma, pancreatic and prostate cancer. Then, the aim of this study is to evaluate the role of CSDE1/UNR in colorectal cancer progression and maintenance of aggressive phenotype. We firstly evaluated UNR/CSDE1 expression in human colon cancer derived cell lines and patient samples. Subsequently, we performed functional experiments by UNR/CSDE1 downregulation. We also evaluated UNR/CSDE1 prognostic relevance in two independent sets of patients. Not only was UNR/CSDE1 expression higher in tumor samples compared to untransformed samples, but also in colonospheres and metastatic origin cell lines than their parental and primary cell lines, respectively. Downregulation of UNR/CSDE1 reduced cell viability and migration throughout a restrain of epithelial-to-mesenchymal transition and increases sensitivity to apoptosis. Interestingly, high UNR/CSDE1 expression was associated with poor prognosis and correlated positively with c-MYC expression in colorectal cancer samples and cell lines. Here, we show for the first time compelling data reporting the oncogenic role of UNR/CSDE1 in human colorectal cancer. View Full-Text
Keywords: UNR; CSDE1; colorectal cancer; metastasis; prognosis biomarker; c-MYC UNR; CSDE1; colorectal cancer; metastasis; prognosis biomarker; c-MYC
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Martinez-Useros, J.; Garcia-Carbonero, N.; Li, W.; Fernandez-Aceñero, M.J.; Cristobal, I.; Rincon, R.; Rodriguez-Remirez, M.; Borrero-Palacios, A.; Garcia-Foncillas, J. UNR/CSDE1 Expression Is Critical to Maintain Invasive Phenotype of Colorectal Cancer through Regulation of c-MYC and Epithelial-to-Mesenchymal Transition. J. Clin. Med. 2019, 8, 560.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J. Clin. Med. EISSN 2077-0383 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top