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Open AccessArticle

Circulating Endometrial Cells: A New Source of Information on Endometriosis Dynamics

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Department of Laboratory Genetics, Laboratory Diagnostics, Faculty Hospital Královské Vinohrady, Srobarova 50, 100 34 Prague 10, Czech Republic
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Department of Gynecology, Military University Hospital and 3rd Faculty of Medicine, U Vojenske nemocnice 1200, 169 02 Prague 6, Czech Republic
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Department of Obstetrics and Gynecology, University Hospital, Faculty of Medicine Charles University, Alej Svobody 80, 301 66 Pilsen, Czech Republic
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Cellpeutics Sp. z o.o., Duńska 9,54-424 Wrocław, Poland
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Department of Oncology, Wroclaw Medical University, Wybrzeże Ludwika Pasteura 1, 50-367 Wrocław, Poland and Wroclaw Comprehensive Cancer Center, Plac Ludwika Hirszfelda 12, 53-413 Wrocław, Poland
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Academic Unit of Obstetrics and Gynecology Ospedale Policlinico San Martino Genoa, Italy Department of Neurosciences, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DiNOGMI) University of Genoa Genoa, Italy Academic Unit of Gynecology and Obstetrics University of Genoa, 16 132 Genoa, Italy
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Department of Histology and Embryology, Wroclaw Medical University, L. Pasteur 1, 503 67 Wroclaw, Poland
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Department of Thoracic Surgery, Krajská zdravotní a.s. Hospital, 41100 Ústí nad Labem, Czech Republic
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3rd Department of Surgery University Hospital FN Motol and 1st Faculty of Medicine Charles University, V Uvalu 84, 150 06 Prague 5, Czech Republic
*
Author to whom correspondence should be addressed.
The authors contributed equally.
J. Clin. Med. 2019, 8(11), 1938; https://doi.org/10.3390/jcm8111938
Received: 28 September 2019 / Revised: 22 October 2019 / Accepted: 4 November 2019 / Published: 11 November 2019
(This article belongs to the Section Obstetrics & Gynecology)
The focus of the presented work was to isolate and characterize circulating endometrial cells (CECs) enriched from peripheral blood (PB) of patients with diagnosed endometriosis. The molecular characteristics of CECs could be supportive for an understanding of endometriosis pathogenesis and treatment decisions in the future. Material and Methods: Blood samples (n = 423) were tested for CECs presence. Subsequently, gene expression analysis (GEA) was carried out for CECs. In parallel, CECs presence and characteristics were tested during menstrual cycle (MC) phases in 11 patients. CECs were enriched by size-based separation. Results: CECs were present in 78.4% of the tested blood samples. In line with the revised American Fertility Society (rAFS) classification, CECs presence was confirmed in all the acknowledged endometriosis stages: minimal, mild, moderate, and severe. Surprisingly, CECs negativity rate was also reported for severe disease in 21.1% of cases. The CECs captured during MC phases displayed different cytomorphology, including epithelial, stromal, and stem cell-like characteristics. The highest CECs numbers were detected in the mid-secretory phase of MC, which corresponds to uterine lining decidualization. CECs captured during mid-secretory periods expressed genes KRT18, NANOG, and VIM in higher amounts when compared to the proliferative phase of MC, where genes KRT19 and ESR1 were mostly elevated. GEA of the super-positive CECs samples (1000 CECs/8 mL PB) revealed high expression of genes KRT18, VIM, NANOG, and FLT1. The expression of these genes was also elevated in the endometriosis tissue samples and endometrioma. Conclusion: The panel of the identified CEC genes could be tested in a prospective manner to confirm the role of CECs in endometriosis pathogenesis and diagnostics. View Full-Text
Keywords: circulating endometrial cells; endometriosis; rare cells; menstrual cycle; liquid biopsy circulating endometrial cells; endometriosis; rare cells; menstrual cycle; liquid biopsy
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MDPI and ACS Style

Pospisilova, E.; Kiss, I.; Souckova, H.; Tomes, P.; Spicka, J.; Matkowski, R.; Jedryka, M.; Ferrero, S.; Bobek, V.; Kolostova, K. Circulating Endometrial Cells: A New Source of Information on Endometriosis Dynamics. J. Clin. Med. 2019, 8, 1938.

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