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Comment from the Editors on the Special Issue: Advanced Analytical Methods in Clinical Diagnosis and Therapy
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Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives

1
Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, Sydney 2109, Australia
2
QIMR Berghofer Medical Research Institute, Brisbane QLD 4006, Australia
3
Southwestern Academic Limb Salvage Alliance (SALSA), Keck School of Medicine of University of Southern California (USC), Los Angeles, CA 90007, USA
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2019, 8(11), 1935; https://doi.org/10.3390/jcm8111935
Received: 17 October 2019 / Revised: 6 November 2019 / Accepted: 8 November 2019 / Published: 10 November 2019
(This article belongs to the Section Infectious Diseases)
Diabetic foot ulcers (DFUs) and diabetic foot infections (DFIs) are associated with reduced patient quality of life, lower-extremity amputation, hospitalization, and high morbidity and mortality. Diverse bacterial communities have been identified in DFUs/DFIs, playing a significant role in infection prognosis. However, due to the high heterogeneity of bacterial communities colonized in DFUs/DFIs, culture-based methods may not isolate all of the bacterial population or unexpected microorganisms. Recently, high sensitivity and specificity of DNA (metagenomics) and RNA (metatranscriptomics) technologies have addressed limitations of culture-based methods and have taken a step beyond bacterial identification. As a consequence, new advances obtained from DNA- and RNA-based techniques for bacterial identification can improve therapeutic approaches. This review evaluated the current state of play in aetiology of DFUs/DFIs on culture and molecular approaches, and discussed the impact of metagenomic and metatranscriptomic methods in bacterial identification approaches. View Full-Text
Keywords: diabetic foot ulcers; diabetic foot infections; microbiology; culture; culturomics; 16S rRNA sequencing; microbiota; metagenomics; metatranscriptomics diabetic foot ulcers; diabetic foot infections; microbiology; culture; culturomics; 16S rRNA sequencing; microbiota; metagenomics; metatranscriptomics
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MDPI and ACS Style

Sadeghpour Heravi, F.; Zakrzewski, M.; Vickery, K.; G. Armstrong, D.; Hu, H. Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives. J. Clin. Med. 2019, 8, 1935.

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