Next Article in Journal
Nitric Oxide and Biological Mediators in Pediatric Chronic Rhinosinusitis and Asthma
Next Article in Special Issue
Modular Strategies to Build Cell-Free and Cell-Laden Scaffolds towards Bioengineered Tissues and Organs
Previous Article in Journal
Histopathological Verification of the Diagnostic Performance of the EU-TIRADS Classification of Thyroid Nodules—Results of a Multicenter Study Performed in a Previously Iodine-Deficient Region
Previous Article in Special Issue
3D Bioprinted Human Cortical Neural Constructs Derived from Induced Pluripotent Stem Cells
Open AccessReview

Induced Pluripotent Stem Cells as Vasculature Forming Entities

CESMA—Centro Servizi Metrologici e Tecnologici Avanzati, University of Naples Federico II, 80146 Naples, Italy
Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, I-80137 Naples, Italy
Interdepartmental Center for Research in Biomaterials (CRIB) University of Naples Federico II, I-80125 Naples, Italy
School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, MC, Italy
Center for Advanced Biomaterials for Healthcare, Istituto Italiano di Tecnologia, 80125 Naples, Italy
Author to whom correspondence should be addressed.
J. Clin. Med. 2019, 8(11), 1782;
Received: 27 September 2019 / Revised: 21 October 2019 / Accepted: 23 October 2019 / Published: 25 October 2019
(This article belongs to the Special Issue Cutting Edge Preclinical Models in Translational Medicine)
Tissue engineering (TE) pursues the ambitious goal to heal damaged tissues. One of the most successful TE approaches relies on the use of scaffolds specifically designed and fabricated to promote tissue growth. During regeneration the guidance of biological events may be essential to sustain vasculature neoformation inside the engineered scaffold. In this context, one of the most effective strategies includes the incorporation of vasculature forming cells, namely endothelial cells (EC), into engineered constructs. However, the most common EC sources currently available, intended as primary cells, are affected by several limitations that make them inappropriate to personalized medicine. Human induced Pluripotent Stem Cells (hiPSC), since the time of their discovery, represent an unprecedented opportunity for regenerative medicine applications. Unfortunately, human induced Pluripotent Stem Cells-Endothelial Cells (hiPSC-ECs) still display significant safety issues. In this work, we reviewed the most effective protocols to induce pluripotency, to generate cells displaying the endothelial phenotype and to perform an efficient and safe cell selection. We also provide noteworthy examples of both in vitro and in vivo applications of hiPSC-ECs in order to highlight their ability to form functional blood vessels. In conclusion, we propose hiPSC-ECs as the preferred source of endothelial cells currently available in the field of personalized regenerative medicine. View Full-Text
Keywords: induced pluripotent stem cells; tissue engineering; angiogenesis; tissue regeneration; from bench to bedside induced pluripotent stem cells; tissue engineering; angiogenesis; tissue regeneration; from bench to bedside
Show Figures

Figure 1

MDPI and ACS Style

Palladino, A.; Mavaro, I.; Pizzoleo, C.; De Felice, E.; Lucini, C.; de Girolamo, P.; Netti, P.A.; Attanasio, C. Induced Pluripotent Stem Cells as Vasculature Forming Entities. J. Clin. Med. 2019, 8, 1782.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop