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Open AccessArticle

Assessment of the Pro12Ala Polymorphism in the PPAR-γ2 Gene among Type 2 Diabetes Patients in a Nigerian Population

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Department of Chemical Sciences, Godfrey Okoye University, Thinkers Corner, Enugu P.M.B 01014, Nigeria
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Department of Biotechnology, Faculty of Science, Ebonyi State University, Abakaliki P.M.B 53, Nigeria
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Department of Biotechnology, Federal University, Ndufu-Alike Ikwo (FUNAI), Abakaliki P.M.B. 1010, Nigeria
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Department of Microbiology, Godfrey Okoye University, Thinkers Corner, Enugu P.M.B 01014, Nigeria
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Diabetes Research Group, Department of Biology, Chemistry and Forensic Science, School of Science, Faculty of Science, University of Wolverhampton, Wulfruna Street, WV1 1LY Wolverhampton, UK
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2018, 7(4), 69; https://doi.org/10.3390/jcm7040069
Received: 7 March 2018 / Revised: 24 March 2018 / Accepted: 2 April 2018 / Published: 5 April 2018
(This article belongs to the Section Molecular Medicine)
The association between the Pro12Ala polymorphism of the PPARγ2 gene, type 2 diabetes (T2D), and obesity in certain ethnic populations has been reported. However, this relationship has not yet been described among diabetes patients in Nigeria. This study investigated the relationship between the Pro12Ala polymorphism in the PPARγ2 gene, obesity, and lipid abnormalities characterizing T2D among patients in Nigeria. This case-control study recruited 73 T2D and 75 non-diabetic (ND) patients. Demographic and clinical data were collected and blood glucose levels together with serum lipid profile for patients were measured. Pro12Ala polymorphism in the PPARγ2 gene was genotyped by restriction fragment length-Polymerase Chain Reaction (RFLP-PCR). The PPAR-γ2 gene (amplicon size = 270 base pair) was successfully amplified for all samples. Following restriction enzyme digestion and analysis by agarose gel electrophoresis, amplicons from samples showed a band of size 270 bp and were of the wild homozygous Pro/Pro genotype. Ala12 variant was totally absent from the study population. Obesity, estimated using Body Mass Index (BMI) and waist circumference (WC), was significantly higher (p < 0.05) in T2D patients compared to the non-diabetic patients. More so, the prevalence of lipid abnormalities; hypercholesterolaemia (TC > 200 mg/dL), hypertriglyceridaemia (TG > 150 mg/dL), high HDL (>100 mg/dL), and low HDL (<50 mg/dL) was significantly greater (p < 0.001) in T2D patients compared to non-diabetic patients. Results obtained further indicated lack of significant association between PPAR-γ2 gene polymorphism, T2D, and obesity. However, obesity and dyslipidaemia were strongly associated in T2D patients. View Full-Text
Keywords: type 2 diabetes; PPAR-γ2 gene; Pro12Ala polymorphism; obesity; lipid profile indices; Nigeria; genetic association type 2 diabetes; PPAR-γ2 gene; Pro12Ala polymorphism; obesity; lipid profile indices; Nigeria; genetic association
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Engwa, G.A.; Nwalo, F.N.; Chiezey, V.O.; Unachukwu, M.N.; Ojo, O.O.; Ubi, B.E. Assessment of the Pro12Ala Polymorphism in the PPAR-γ2 Gene among Type 2 Diabetes Patients in a Nigerian Population. J. Clin. Med. 2018, 7, 69.

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