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Amiloride Promotes Oligodendrocyte Survival and Remyelination after Spinal Cord Injury in Rats

Department of Orthopaedic Surgery, Surgical Science Tokai University School of Medicine 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan
Author to whom correspondence should be addressed.
J. Clin. Med. 2018, 7(3), 46;
Received: 29 December 2017 / Revised: 22 February 2018 / Accepted: 2 March 2018 / Published: 5 March 2018
(This article belongs to the Special Issue Injury and Repair in the Nervous System)
After spinal cord injury (SCI), secondary injury results in an expanding area of glial cell apoptosis. Oligodendrocyte precursor cells (OPCs) actively proliferate after SCI, but many of these cells undergo apoptosis. One of the factors that exacerbates secondary injury is endoplasmic reticulum (ER) stress. In this study, we tested the effects of amiloride treatment on the fate of OPCs during secondary injury in rats. Amiloride is an FDA-approved diuretic for treating hypertension, which in rats enhances ER stress response and suppresses the apoptosis of glial cells after SCI. A severe contusive SCI was induced in Sprague-Dawley rats using an infinite horizon (IH)-impactor (200 kdyne). Beginning 24 h after SCI, 10 mg/kg of amiloride or phosphate buffered saline (PBS) was intraperitoneally administered daily for a period of 14 days. At 7, 14, 28, and 56 days after SCI, animals were subsequently euthanized in order to analyze the injured spinal cord. We labeled proliferating OPCs and demonstrated that amiloride treatment led to greater numbers of OPCs and oligodendrocytes in the injured spinal cord. Increased myelin basic protein (MBP) expression levels were observed, suggesting that increased numbers of mature oligodendrocytes led to improved remyelination, significantly improving motor function recovery. View Full-Text
Keywords: spinal cord injury; amiloride; oligodendrocyte precursor cell spinal cord injury; amiloride; oligodendrocyte precursor cell
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Imai, T.; Katoh, H.; Suyama, K.; Kuroiwa, M.; Yanagisawa, S.; Watanabe, M. Amiloride Promotes Oligodendrocyte Survival and Remyelination after Spinal Cord Injury in Rats. J. Clin. Med. 2018, 7, 46.

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