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Open AccessBrief Report

Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis

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Clinical HIV Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of Witwatersrand, Johannesburg 2092, South Africa
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Center for Biostatistics in AIDS Research, Harvard T.H. Chan School of Public Health Boston, Boston, MA 02115, USA
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Instituto Nacional de Infectologia Evandro Chagas-INI-Fiocruz, Rio de Janeiro 21.045-900, Brazil
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Pulmonary and Critical Care Department, Baylor Scott and White Hospital, Temple, TX 76508, USA
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Social & Scientific Systems, Inc., Silver Spring, MD 20910, USA
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Cepheid Inc., Sunnyvale, CA 94089, USA
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Division of Infectious Disease, Rutgers-New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USA
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Division of Tuberculosis Elimination, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
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The Veterans Affairs Medical Center, The George Washington University, Washington, DC 20422, USA
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Division of HIV, Infectious Diseases and Global Medicine, Zuckerberg San Francisco General, University of California, San Francisco, CA 94110, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Mehdi Mirsaeidi
J. Clin. Med. 2017, 6(8), 78; https://doi.org/10.3390/jcm6080078
Received: 23 May 2017 / Revised: 20 July 2017 / Accepted: 31 July 2017 / Published: 7 August 2017
(This article belongs to the Special Issue Tuberculosis Treatment and Management)
As a strategy to improve the sensitivity of nucleic acid-based testing in acid-fast bacilli (AFB) negative samples, larger volumes of sputum (5–10 mL) were tested with Xpert® MTB/RIF from 176 individuals with smear-negative sputum undergoing tuberculosis evaluation. Despite larger volumes, this strategy had a suboptimal sensitivity of 50% (4/8). View Full-Text
Keywords: larger volume sputum; Xpert MTB/RIF; smear-negative suspects larger volume sputum; Xpert MTB/RIF; smear-negative suspects
MDPI and ACS Style

Badal-Faesen, S.; Firnhaber, C.; Kendall, M.A.; Wu, X.; Grinsztejn, B.; Escada, R.O.S.; Fernandez, M.; Hogg, E.; Sanne, I.; Johnson, P.; Alland, D.; Mazurek, G.H.; Benator, D.A.; Luetkemeyer, A.F.; The AIDS Clinical Trials Group A5295; Tuberculosis Trials Consortium Study 34 Teams. Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis. J. Clin. Med. 2017, 6, 78.

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