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J. Clin. Med. 2017, 6(8), 78;

Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis

Clinical HIV Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of Witwatersrand, Johannesburg 2092, South Africa
Center for Biostatistics in AIDS Research, Harvard T.H. Chan School of Public Health Boston, Boston, MA 02115, USA
Instituto Nacional de Infectologia Evandro Chagas-INI-Fiocruz, Rio de Janeiro 21.045-900, Brazil
Pulmonary and Critical Care Department, Baylor Scott and White Hospital, Temple, TX 76508, USA
Social & Scientific Systems, Inc., Silver Spring, MD 20910, USA
Cepheid Inc., Sunnyvale, CA 94089, USA
Division of Infectious Disease, Rutgers-New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USA
Division of Tuberculosis Elimination, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
The Veterans Affairs Medical Center, The George Washington University, Washington, DC 20422, USA
Division of HIV, Infectious Diseases and Global Medicine, Zuckerberg San Francisco General, University of California, San Francisco, CA 94110, USA
Authors to whom correspondence should be addressed.
Academic Editor: Mehdi Mirsaeidi
Received: 23 May 2017 / Revised: 20 July 2017 / Accepted: 31 July 2017 / Published: 7 August 2017
(This article belongs to the Special Issue Tuberculosis Treatment and Management)
Full-Text   |   PDF [200 KB, uploaded 7 August 2017]


As a strategy to improve the sensitivity of nucleic acid-based testing in acid-fast bacilli (AFB) negative samples, larger volumes of sputum (5–10 mL) were tested with Xpert® MTB/RIF from 176 individuals with smear-negative sputum undergoing tuberculosis evaluation. Despite larger volumes, this strategy had a suboptimal sensitivity of 50% (4/8). View Full-Text
Keywords: larger volume sputum; Xpert MTB/RIF; smear-negative suspects larger volume sputum; Xpert MTB/RIF; smear-negative suspects
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Badal-Faesen, S.; Firnhaber, C.; Kendall, M.A.; Wu, X.; Grinsztejn, B.; Escada, R.O.S.; Fernandez, M.; Hogg, E.; Sanne, I.; Johnson, P.; Alland, D.; Mazurek, G.H.; Benator, D.A.; Luetkemeyer, A.F.; the AIDS Clinical Trials Group A5295; Tuberculosis Trials Consortium Study 34 Teams. Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis. J. Clin. Med. 2017, 6, 78.

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