1. Introduction
The prevalence of overweight and obesity has become a public health concern around the world [
1]. According to the World Health Organization’s 2010 report, 35% of adults aged 20 years and older worldwide were clinically overweight (body mass index, BMI ≥ 25 kg/m
2) based on data from 2008. More than half a billion adults in the same category were obese (BMI ≥ 30 kg/m
2) [
2]. Moreover, 2.8 million deaths are linked to raised BMI each year [
2]. Overweight and obesity are associated with an increased risk of developing an assortment of metabolic diseases, which mainly include hypertension, type 2 diabetes mellitus, non-alcoholic fatty liver disease, coronary heart disease, and stroke [
1,
3]. The implications of all those health conditions have brought gloomy biomedical and socioeconomic consequences to the well-being of the global population [
3].
Both genetic and nutritional/environmental factors are instrumental to the development of obesity and its related metabolic diseases [
4,
5]. Mutations in the appetite-regulating hormones (e.g., leptin and proopiomelanocortin) and their corresponding receptors (e.g., leptin receptor and melanocortin 4 receptor, respectively) result in early onset of obesity and obesity-associated metabolic dysregulation in both humans and experimental rodent models [
6]. Given the increasing numbers of the monogenic contributors, the majority of the obesity cases in the human population are believed to be polygenic [
6]. On the other hand, overnutrition related to unhealthy eating habits probably drives the rising global prevalence of overweight and obesity [
7,
8]. In spite of the self-evident link between overnutrition and obesity, the underlying mechanisms that cause obesity and its related metabolic diseases due to excessive intake of nutrients have not been fully understood.
The utilization of dietary macronutrients requires coordinative regulation of the metabolic pathways in different organs and tissues of the body. In a fed state, excessive dietary macronutrients are conserved in the form of glycogen and triglycerides (TG) for future energy needs. During fasting, glycogen and TG are, respectively, broken down into glucose and fatty acids (FAs) to meet the energy needs of different organs and tissues in the body. These processes are coordinately regulated by both hormonal and nutritional stimuli in response to feeding and energy states so that the body reaches energy homeostasis [
4,
5]. Dysregulated hormonal balance and nutrient metabolism not only disrupt the energy homeostasis, but also predispose individuals to developing obesity and related metabolic diseases [
5].
Insulin, secreted from pancreatic β-cells in response to physiological stimuli, is an important peptide hormone in anabolism [
9]. Upon secretion, it binds to the insulin receptor on cell membranes and exerts its action through a signal transduction cascade that is mainly composed of kinases and phosphatases. The binding causes conformational changes of the insulin receptor and transphosphorylation of the β-subunit tyrosine kinase [
10,
11], which initiates the signal transduction cascade [
12]. The signal is transduced by multiple components in a complex network containing multiple kinases and phosphatases [
13,
14]. The activation of the insulin signaling pathway results in changes in the activities and/or the expression levels of enzymes involved in nutrient metabolism. These subsequently alter the metabolic state of tissues and organs in the body. For example, insulin promotes glycolysis, glycogenesis, and lipogenesis, and, at the same time, suppresses gluconeogenesis in the liver [
14]. However, in overweight and obese individuals, a certain dose of insulin only produces subnormal physiological responses. This observation is regarded as insulin resistance, which occurs concurrently with profound alterations in glucose and lipid metabolism [
9,
15,
16]. Even though the molecular mechanisms leading to insulin resistance have not been fully understood, it is postulated that systemic inflammation, dysregulated lipid metabolism, and gastrointestinal dysbiosis, triggered by overnutrition, interplay with each other and result in the impairment of insulin action [
16].
Overnutrition not only provides excessive energy from macronutrients, but also superfluous amounts of vitamins and essential factors, which have regulatory roles. How micronutrients, such as vitamin A (VA; retinol), regulate the homeostasis of macronutrients is still an ongoing research topic. As an essential and lipophilic micronutrient, VA plays a key role in the general health of an individual [
5]. This review summarizes recent research progresses regarding the role of VA in carbohydrate, lipid and protein metabolism in the liver, pancreas, skeletal muscle, and adipose tissues, which are major active players in glucose and FA metabolism.
2. Overview of VA and Its Metabolism
VA is important for a myriad of physiological functions, including vision formation, immune response, cell differentiation and proliferation, embryonic development and metabolism [
17]. The investigation of its pleiotropic effect on animals resulted in the discovery of the retinoid signaling pathway by which VA exerts its function by modulating gene expression in target cells [
18,
19]. Dietary molecules with VA activities exist in two forms: provitamin A (carotenoids and cryptoxanthins) and preformed VA (retinol or retinyl esters). Provitamin A molecules are mainly found in plants. Herbivorous and omnivorous animals convert provitamin A molecules into retinol before storing it as retinyl esters in different organs and tissues, depending on the species [
20]. Retinol and retinyl esters are then introduced into the food chain and transferred to carnivorous and omnivorous animals at the higher tiers of the food chain [
21].
After consumption (
Figure 1), dietary retinol, retinyl esters, and carotenoids are released from the digested food matrix in the gastrointestinal tract. In the intestinal lumen, the brush-border retinyl ester hydrolase hydrolyzes retinyl esters into retinol and free FAs [
22,
23]. Due to their lipophilic characteristic, these molecules are incorporated into micelles in the intestinal lumen prior to absorption. Carotenoids enter enterocyte (intestinal mucosal cell) via passive diffusion, which neither requires cell membrane transporters nor energy [
24,
25]. Retinol is thought to be taken up into enterocytes by a brush border membrane transporter via facilitated diffusion [
26].
Inside the enterocytes, the majority of β-carotene (major dietary carotenoid) will be symmetrically cleaved by 15,15′-dioxygenase to generate retinal [
27]. Residual intact β-carotene can be delivered to the liver, where the same enzyme in hepatocytes catalyzes the cleavage reaction [
25]. Aside from the symmetrical cleavage, β-carotene can also be asymmetrically cleaved at the 9′,10′ double bond by a specific enzyme in the enterocyte [
28,
29]. The resulting retinal from the cleavage of the β-carotene is readily reduced to retinol, which converges into the retinol repertoire from the hydrolysis of retinyl esters [
30]. The majority of the retinol in the enterocyte is re-esterified into retinyl ester by lecithin:retinol acyltransferase [
31] (LRAT) or acyl-CoA:retinol acyltransferase [
32] (ARAT), and then incorporated into chylomicrons for delivery [
30].
Figure 1.
Schematic representation of vitamin A (VA) digestion, absorption, transport, and metabolism in the body. Dietary VA from plant and animal sources is digested in the intestinal lumen and absorbed by enterocytes via different mechanisms. Within the enterocytes, the dietary VA is converted into retinyl esters, which are packaged into chylomicrons for the secretion into the lymph and eventually enter the circulation. The lipoprotein lipase hydrolyzes triglycerides (TGs) on the chylomicrons to produce chylomicron remnants. The retinyl ester-containing chylomicron remnants are eventually taken up by hepatocytes, where the retinyl esters are again hydrolyzed into retinol. The released retinol can be transported to target cells and catabolized into retinal, retinoic acid (RA), or other metabolites. Excessive retinol is re-esterified into retinyl esters, which are stored in stellate cells. BB-REH, brush-border retinyl ester hydrolase; LRAT, lecithin:retinol acyltransferase; ARAT, acyl-CoA:retinol acyltransferase; RBP, retinol binding protein; RDH, retinol dehydrogenase; RALDH, retinal dehydrogenase; REH, retinol ester hydrolase; STRA6, stimulated by retinoic acid 6; RA, retinoic acid; RARE, retinoic acid responsive element; RAR, retinoic acid receptor; RXR, retinoid X receptor; TTR, transthyretin; PPAR, peroxisome proliferator-activated receptor; HNF4α, hepatocyte nuclear factor 4α; COUP-TFII, chicken ovalbumin up-stream transcription factor II.
Figure 1.
Schematic representation of vitamin A (VA) digestion, absorption, transport, and metabolism in the body. Dietary VA from plant and animal sources is digested in the intestinal lumen and absorbed by enterocytes via different mechanisms. Within the enterocytes, the dietary VA is converted into retinyl esters, which are packaged into chylomicrons for the secretion into the lymph and eventually enter the circulation. The lipoprotein lipase hydrolyzes triglycerides (TGs) on the chylomicrons to produce chylomicron remnants. The retinyl ester-containing chylomicron remnants are eventually taken up by hepatocytes, where the retinyl esters are again hydrolyzed into retinol. The released retinol can be transported to target cells and catabolized into retinal, retinoic acid (RA), or other metabolites. Excessive retinol is re-esterified into retinyl esters, which are stored in stellate cells. BB-REH, brush-border retinyl ester hydrolase; LRAT, lecithin:retinol acyltransferase; ARAT, acyl-CoA:retinol acyltransferase; RBP, retinol binding protein; RDH, retinol dehydrogenase; RALDH, retinal dehydrogenase; REH, retinol ester hydrolase; STRA6, stimulated by retinoic acid 6; RA, retinoic acid; RARE, retinoic acid responsive element; RAR, retinoic acid receptor; RXR, retinoid X receptor; TTR, transthyretin; PPAR, peroxisome proliferator-activated receptor; HNF4α, hepatocyte nuclear factor 4α; COUP-TFII, chicken ovalbumin up-stream transcription factor II.

In the circulation, retinyl ester-containing chylomicrons interact with the lipoprotein lipase at the inner lining of endothelial cells of the vascular system, which gradually hydrolyzes the TGs on the chylomicrons to free FAs. This process decreases the size of the chylomicrons and converts them into chylomicron remnants. The retinyl ester-containing chylomicron remnants are eventually taken up by hepatocytes, where retinyl esters are again hydrolyzed into retinol. The released retinol can be transported to target cells and catabolized into retinal, retinoic acid (RA), or other metabolites for the different physiological functions. Excessive retinol is re-esterified into retinyl esters, which are stored in stellate cells inside the liver [
5]. A combination of retinol and RA is shown to increase retinol uptake and esterification in the lung [
33]. A portion of retinyl esters is absorbed by extrahepatic tissues [
34].
Retinol derived from the liver is associated with retinol binding protein 4 (RBP4) and transthyretin (TTR) as a complex for the delivery to extrahepatic tissues. The uptake of retinol in the holo-RBP4-TTR complex is supposedly mediated by a receptor on the cell’s membrane. Stimulated by retinoic acid gene 6 (STRA6), a membrane receptor protein, is identified from bovine retinal pigment epithelium cells due to its high affinity to bind to RBPs [
35]. Mutations in the human
STRA6 gene cause Matthew-Wood Syndrome [
36,
37]. However, the deletion of the
Stra6 gene in mice does not cause any apparent developmental or growth defect [
38,
39,
40]. Interestingly, other than retinal pigment endothelium abnormalities [
38,
39,
40], the levels of retinol and retinyl esters in the plasma and other tissues in
Stra6−/− mice are similar to those in wild type control mice [
38].
Stra6−/− mice have normal glucose tolerance [
38,
39]. When
Stra6−/− mice were fed a VA deficient diet for 5 weeks, they were shown to have less retinol and retinyl ester levels in the white adipose tissue, kidney, heart, and testis than wild type control mice, indicating that STRA6 is responsible for part of the retinol uptake in these tissues [
38]. The deletion of
Stra6 in mice reduces insulin resistance mediated by the injection of holo-RBP and feeding of a high-fat diet, suggesting that STRA6 functions as a receptor to mediate RBP-induced insulin resistance [
38]. These results indicate that STRA6 is not the only pathway for retinol transport, and other transporters or mechanisms may exist for retinol uptake in cells.
Many of VA’s physiological functions are mediated by RA. The generation and disposal of RA are highly regulated processes involving complex retinoid binding proteins and metabolic enzymes [
41,
42]. In essence, retinol can be reversibly oxidized into retinal by retinol dehydrogenases (RDHs), and further irreversibly oxidized into RA by retinal dehydrogenases (RALDHs), as reviewed in [
41].
In vitro biochemical experiments have so far identified almost a dozen enzymes with retinol oxidation capabilities, but only RDH1, RDH10, and dehydrogenase/reductase member 9 (DHRS9) are verified and supported by
in vivo evidence [
41]. Comparatively, four RALDHs have been identified, which are expressed and regulated in a tissue specific manner [
41]. RALDH1 is universally expressed and accounts for over 90% of retinal oxidation capability in rat liver and kidney [
43]. Despite its ubiquitous expression profile, RALDH1 knockout mice do not manifest developmental differences compared to their wild type counterparts [
44]. Interestingly, RALDH1 knockout mice are resistant against high-fat diet induced glucose intolerance and obesity [
45,
46]. In contrast to RALDH1, RALDH2 knockout mice are embryonically lethal. This suggests that RALDH2 is responsible for the local production of RA during embryo development [
47]. RALDH3 does not recognize 9-
cis retinal as a substrate, whereas RALDH4 only recognizes 9-
cis retinal as a substrate [
48,
49]. The selective recognition of substrates suggests distinctly different functions of the two enzymes
in vivo. However, more research is necessary to elucidate the underlying mechanisms [
48,
49]. The catabolic cytochrome P450 (CYP) family of enzymes partially contributes to RA homeostasis. These enzymes require NADPH and dioxygen to convert RA into a myriad of end products, including 4-hydroxy-RA, 4-oxo-RA, and 18-hydroxy-RA [
50]. A representative member of the family, CYP26A1, was cloned and investigated for its role in the regulation of RA homeostasis in animals [
51,
52,
53]. CYP26A1 levels are low in VA deficient animals, but CYP26A1 is substantially induced by RA or a VA sufficient diet [
52]. CYP26A1 null mice are embryonically lethal, showing phenotypes similar to wild type mice treated with excessive RA [
54]. These data implicate an autoregulatory mechanism by which CYP26A1 controls RA catabolism to maintain RA levels
in vivo.
After RA molecule synthesis, they translocate to the nucleus, where they bind to and activate two subfamilies of nuclear receptors known as the retinoid acid receptors (RARs) and retinoid X receptors (RXRs). Nuclear receptors are ligand-activated transcription factors involved in a variety of physiological processes [
55]. RARs and RXRs each contain three isoforms that have different tissue and developmental expression profiles [
55]. It has been thought that all-
trans RA is an exclusive ligand for RARs, whereas 9-
cis RA is a common ligand for both RARs and RXRs [
56]. Transcription factors mediating RA activity can recognize short consensus sequences, which are termed retinoic acid response elements (RAREs), located in the promoter region of the RA responsive genes. Depending on the specific gene, RXR can bind to RARE as a homodimer or a heterodimer with RAR or other nuclear receptors [
19]. As shown in
Figure 1, in addition to RARs and RXRs, RA signals have been suggested to be mediated by other nuclear receptors, such as peroxisome proliferator-activated receptor (PPAR) β/δ, hepatocyte nuclear factor 4α, and chicken ovalbumin up-stream transcription factor II [
5,
57]. The binding of their specific ligands to the nuclear receptors will help recruit corresponding transcriptional coactivators and dissociate transcriptional corepressors in the general transcription machinery, leading to the regulation of RA-targeted gene expression.
3. VA and Growth, Appetite, Taste and Olfaction
VA was first recognized as an essential factor for animal growth. Following the withdrawal of VA from the diet, growth retardation is the earliest and most reproducible sign of deficiency in many experimental animals [
58]. Rats fed on a VA deficient diet upon weaning (about three weeks old), manifest cessation of growth and significant weight loss later on [
59,
60]. The reduction of body mass can be prevented by RA supplementation. However, once RA is removed from those rats fed on the VA deficient diet, the weight loss will reappear in a couple of days [
61,
62]. These results demonstrate that VA is important for normal growth and development of animals.
In comparison to rats, mice are more resistant to the development of VA deficiency. A normal growth curve is usually observed in weaning mice with sufficient hepatic VA storage, despite being sustained on a VA deficient diet [
63,
64]. In one study, a significant weight loss was achieved in mice fed on a VA deficient diet only when the VA storage was pre-depleted prior to weaning [
63]. This suggests that mice might have special mechanisms to maintain VA homeostasis or they might use VA more efficiently. As a result, experimental conclusions obtained in mice cannot be directly extrapolated to rats or humans in terms of VA and its effect on growth and metabolism.
VA deficient animals exhibit decreased appetite and food intake during the period of growth cessation [
59,
60]. The underlying molecular mechanisms by which VA regulates energy intake remain elusive. Since leptin, an adipocyte-derived peptide hormone, exerts its action in the brain to regulate appetite, energy expenditure, and metabolism [
65], some researchers investigated the effects of VA on the expression of leptin in rodents. The results showed that both RA treatment and VA supplementation were able to decrease leptin mRNA in adipocytes, which, in turn, decreased serum leptin levels [
66,
67]. Interestingly, the energy intake of these rodents did not change during the course of VA supplementation [
68]. These data suggest that the VA-induced decrease in leptin levels does not correlate with food intake in these animals [
68].
VA has also been implicated in the normal functions of the taste and olfactory system. VA depletion causes the loss of both preference to sodium chloride and aversion to quinine in rats, and these abnormal responses could be restored by VA supplementation [
69]. One study also shows that the response to sweetness in VA deficient rats is impaired [
70]. Furthermore, VA supplementation can improve the impairment of taste and olfaction in patients with cirrhosis [
71]. Despite these many observations, the mechanism is not clear. Keratin infiltration into the taste buds is proposed to be a mechanism by which VA deficiency affects taste in rats [
69]. But the physical change could not be confirmed in all VA deficient rodent models [
70].
4. VA and Plasma Parameters: Clinical Evidence
The association between VA and metabolism was discovered from clinical observations in human subjects with type 2 diabetes. Biopsies of the liver of diabetic patients showed that the hepatic VA content was twice as much compared with healthy individuals [
72]. Independent studies carried out more than eighty years ago also found that about 85% of adult patients with type 2 diabetes had elevated plasma carotene levels, and more than 10% were clinically diagnosed with xanthosis [
73,
74]. Similar results were obtained to a lesser degree in type 2 diabetic children, but their plasma VA levels were found to be subnormal [
75]. Additionally, reduction of serum retinol and RBP levels was found in type 1 diabetic patients [
76]. These data collectively suggest that VA plays important roles in metabolic homeostasis.
Recently, RBP4, a serum retinol transporter, was shown to be elevated in insulin resistant and type 2 diabetic subjects [
77,
78,
79]. Multiple single nucleotide polymorphisms (SNP) of RBP4 were also discovered in the human genome, which predict the susceptibility to insulin resistance, obesity, and type 2 diabetes [
80,
81]. Despite suggesting a positive association, several clinical studies did not identify significant associations between changes in RBP4 levels and metabolic diseases [
82,
83,
84]. It is worth to note that elevation of plasma RBP4 levels reduced insulin sensitivity in mice, and RBP4 knockout mice had improved insulin sensitivity [
85]. When fenretinide, a synthetic retinoid derivative, was used to disrupt the association of RBP4 and TTR, insulin sensitivity in diet-induced obese mice was improved [
85]. However, when a specific compound was developed to disrupt the RBP4 and TTR interaction and successfully reduced the plasma RBP4 level, insulin sensitivity in the insulin resistant mice was not improved, suggesting that another mechanism may be responsible for the fenretinide-mediated improvement of insulin sensitivity in mice [
86]. Moreover, the improvement of insulin sensitivity in RBP4 knockout mice could not be observed in this study [
86]. Further studies are needed to understand the role of VA in insulin resistance.
The association between VA and plasma lipid metabolism was also observed in patients taking retinoid drugs. Medical administration of isotretinoin (13-
cis RA) results in hypertriglyceridemia in human subjects with acne [
87]. Treatment of patients with acute promyelocytic leukemia with all-
trans RA leads to weight gain and elevation of plasma TG and cholesterol levels [
88,
89]. It is postulated that the dysregulated plasma lipid levels are caused by the RA-induced apolipoprotein CIII expression [
90]. Apolipoprotein CIII is regarded as an inhibitor of lipoprotein lipase activity [
91]. On the contrary, long-term administration of fenretinide, a synthetic retinoid drug currently in phase II clinical trial evaluation, could prevent diet-induced obesity, insulin resistance, and hepatosteatosis [
92,
93]. As more and more retinoid drugs are synthesized and tested, it would be interesting to see how they affect human metabolism in any given health condition.
6. VA and Islets of Langerhans in the Pancreas
Islets of Langerhans, which account for less than 2% of the total pancreas mass, produce and secrete peptide hormones from five different types of specialized cells, including α-, β-, polypeptide (PP-), δ-, and ε-cells [
138,
139]. Among all the hormones produced by islets, glucagon from α-cells and insulin from β-cells are of great clinical interest due to their concerted roles in the regulation of blood glucose levels. Glucagon promotes hepatic glucose production, glycogenolysis, and ketone production in response to hypoglycemia, whereas insulin promotes glucose disposal, glycogenesis, and lipogenesis in response to the increase of plasma glucose levels [
140].
The secretion of glucagon is controlled by the autonomous nervous system, direct action of glucose on α-cells, and indirect effects of paracrine factors from non-α-cells on α-cells [
141]. On the other hand, the secretion of insulin is mainly stimulated by the influx of glucose into β-cells. Glucose metabolism in β-cells leads to elevated ATP/ADP ratios, which in turn, inhibit the ATP-sensitive potassium channels on the cell membrane. The inhibition depolarizes the plasma membrane, which results in the secretion of insulin. In addition to glucose, amino acids and neural stimuli can also stimulate insulin secretion [
142].
It has been shown that VA and its metabolites can affect the secretion of glucagon. In VA deficient rats, impaired glucagon secretion occurs from early stages of deficiency, and the impairment cannot be rescued by RA replenishment [
143]. This demonstrates a critical physiologic role of VA in the normal function of α-cells. Interestingly, in the isolated intact rat islets and glucagon secreting cell lines, both retinol and RA inhibit glucagon secretion in a dose-dependent manner [
144]. This result suggests that the acute effects of VA on glucagon secretion
in vitro do not correlate with its physiological effects
in vivo.
VA deficiency has been shown to decrease β-cell functions in rats in two ways. First, it reduces the β-cell mass in fetal islets by reducing fetal β-cell replication [
145]. Second, it impairs glucose-stimulated insulin secretion (GSIS) from β-cells [
146]. In comparison, the effects of VA and its metabolites on isolated islets and insulin secreting cells are both chemical- and dosage-dependent. For example, retinol at 10
−7 M stimulates GSIS from isolated rat islets, which is in opposition to the inhibitory effect of retinol at 10
−4‒10
−5 M [
147]. Additionally, all-
trans RA potentiates GSIS [
148,
149,
150], whereas 9-
cis RA inhibits GSIS [
151] from rodent islets and INS-1 cells. Cellular retinol-binding protein I knockout mice exhibit increased levels of 9-
cis RA in the pancreas and reduced GSIS [
152]. It is also observed that all-
trans RA increases
Gck mRNA levels and induces enzyme activity of glucokinase [
150], whereas 9-
cis RA treatment is associated with decreased GLUT2 and glucokinase activities in rodent islets [
151]. Given the fact that retinoid receptors require preferential ligands to exert their full function, these results suggest that the production and balance of VA and its metabolites are critical for the normal functioning of β-cells.