The Impact of Obesity on Clostridioides difficile Infection Outcomes: A Retrospective Cohort Study
Abstract
1. Introduction
2. Methods
2.1. Study Setting and Population
2.2. Definitions
2.3. Outcomes
2.4. Microbiological Methods
2.5. Data Collection
2.6. Statistical Analyses
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Formerly, C.; Infection, C.; Gerding, D.N.; Young, V.B.; Donskey, C.J. 243—Clostridioides difficile (Formerly Clostridium difficile) Infection. In Mandell, Douglas, and Bennett’s Principles and Practice of Infectious Diseases, 9th ed.; Elsevier Inc.: Oxford, UK, 2022; pp. 2933–2947.e4. [Google Scholar]
- Kelly, C.P.; Pothoulakis, C.; LaMont, J.T. Clostridium difficile colitis. N. Engl. J. Med. 1994, 330, 257–262. [Google Scholar] [CrossRef]
- Dallal, R.M.; Harbrecht, B.G.; Boujoukas, A.J.; Sirio, C.A.; Farkas, L.M.; Lee, K.K.; Simmons, R. Fulminant Clostridium difficile: An underappreciated and increasing cause of death and complications. Ann. Surg. 2002, 235, 363–372. [Google Scholar] [CrossRef]
- Barbut, F.; Petit, J.C. Epidemiology of Clostridium difficile-associated infections. Clin. Microbiol. Infect. 2001, 7, 405–410. [Google Scholar] [CrossRef] [PubMed]
- Thorpe, C.M.; Gorbach, S.L. Update on Clostridium difficile. Curr. Treat. Options Gastroenterol. 2006, 9, 265–271. [Google Scholar] [CrossRef]
- Kelly, C.P.; LaMont, J.T. Clostridium difficile—More difficult than ever. N. Engl. J. Med. 2008, 359, 1932–1940, Erratum in N. Engl. J. Med. 2010, 363, 1585. [Google Scholar] [CrossRef] [PubMed]
- European Centre for Disease Prevention and Control. Point Prevalence Survey of Healthcare-Associated Infections and Antimicrobial Use in European Acute Care Hospitals, 2016–2017; ECDC: Stockholm, Sweden, 2023. [Google Scholar]
- The National Center for Infection Control of Israel. Available online: https://www.gov.il/he/Departments/DynamicCollectors/infection-control-reports (accessed on 1 January 2025).
- Sullivan, A.; Edlund, C.; Nord, C.E. Effect of antimicrobial agents on the ecological balance of human microflora. Lancet Infect. Dis. 2001, 1, 101–114. [Google Scholar] [CrossRef] [PubMed]
- Manges, A.R.; Labbe, A.; Loo, V.G.; Atherton, J.K.; Behr, M.A.; Masson, L.; Tellis, P.A.; Brousseau, R. Comparative metagenomic study of alterations to the intestinal microbiota and risk of nosocomial Clostridum difficile—Associated disease. J. Infect. Dis. 2010, 202, 1877–1884. [Google Scholar] [CrossRef]
- Hopkins, M.J.; Macfarlane, G.T. Changes in predominant bacterial populations in human faeces with age and with Clostridium difficile infection. J. Med. Microbiol. 2002, 51, 448–454. [Google Scholar] [CrossRef]
- Buffie, C.G.; Pamer, E.G. Microbiota-mediated colonization resistance against intestinal pathogens. Nat. Rev. Immunol. 2013, 13, 790–801. [Google Scholar] [CrossRef]
- Obesity and Overweight—World Health Organization (WHO). Available online: https://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight (accessed on 7 May 2025).
- Boicean, A.; Ichim, C.; Sasu, S.M.; Todor, S.B. Key Insights into Gut Alterations in Metabolic Syndrome. J. Clin. Med. 2025, 14, 2678. [Google Scholar] [CrossRef]
- Crovesy, L.; Masterson, D.; Rosado, E.L. Profile of the gut microbiota of adults with obesity: A systematic review. Eur. J. Clin. Nutr. 2020, 74, 1251–1262. [Google Scholar] [CrossRef]
- Spigaglia, P. Clostridioides difficile and Gut Microbiota: From Colonization to Infection and Treatment. Pathogens 2024, 13, 646. [Google Scholar] [CrossRef]
- El Feghaly, R.E.; Stauber, J.L.; Deych, E.; Gonzalez, C.; Tarr, P.I.; Haslam, D.B. Markers of intestinal inflammation, not bacterial burden, correlate with clinical outcomes in Clostridium difficile infection. Clin. Infect. Dis. 2013, 56, 1713–1721. [Google Scholar] [CrossRef] [PubMed] [PubMed Central]
- Thomas-Dupont, P.; Velázquez-Soto, H.; Izaguirre-Hernández, I.Y.; Amieva-Balmori, M.; Triana-Romero, A.; Islas-Vázquez, L.; Jiménez-Martínez, M.D.C.; Remes-Troche, J.M. Obesity Contributes to Inflammation in Patients with IBS via Complement Component 3 and C-Reactive Protein. Nutrients 2022, 14, 5227. [Google Scholar] [CrossRef] [PubMed]
- Bishara, J.; Farah, R.; Mograbi, J.; Khalaila, W.; Abu-Elheja, O.; Mahamid, M.; Nseir, W. Obesity as a risk factor for Clostridium difficile infection. Clin. Infect. Dis. 2013, 57, 489–493. [Google Scholar] [CrossRef] [PubMed]
- Leung, J.; Burke, B.; Ford, D.; Garvin, G.; Korn, C.; Sulis, C.; Bhadelia, N. Possible association between obesity and Clostridium difficile infection. Emerg. Infect. Dis. 2013, 19, 1791–1798. [Google Scholar] [CrossRef] [PubMed]
- Charoenngam, N.; Ponvilawan, B.; Thongpiya, J.; Yingchoncharoen, P.; Chaikijurajai, T.; Chaisidhivej, N.; Apovian, C.M.; Ungprasert, P. Body mass index and risk of clostridioides difficile infection: A systematic review and meta-analysis. Infection 2022, 50, 725–737. [Google Scholar] [CrossRef] [PubMed]
- Mulki, R.; Baumann, A.J.; Alnabelsi, T.; Sandhu, N.; Alhamshari, Y.; Wheeler, D.S.; Perloff, S.; Katz, P.O. Body mass index greater than 35 is associated with severe Clostridium difficile infection. Aliment. Pharmacol. Ther. 2017, 45, 75–81. [Google Scholar] [CrossRef]
- Chatterjee, T.; Bansal, S.; Abuzar, A.; Hussain, H.; Gupta, L. Is Increased BMI a Risk Factor for Developing Severe Clostridioides Difficile Infection? A Retrospective Study. J. Community Hosp. Intern. Med. Perspect. 2022, 12, 43–50. [Google Scholar] [CrossRef] [PubMed]
- McDonald, L.C.; Gerding, D.N.; Johnson, S.; Bakken, J.S.; Carroll, K.C.; Coffin, S.E.; Dubberke, E.R.; Garey, K.W.; Gould, C.V.; Kelly, C.; et al. Clinical Practice Guidelines for Clostridium difficile Infection in Adults and Children: 2017 Update by the Infectious Diseases Society of America (IDSA) and Society for Healthcare Epidemiology of America (SHEA). Clin. Infect. Dis. 2018, 66, e1–e48. [Google Scholar] [CrossRef]
- Clostridioides Difficle Infection Diagnosis Guidelines of the Israeli Ministry of Health. Available online: https://www.gov.il/he/pages/bz12-2012 (accessed on 7 May 2025).
- Charlson, M.E.; Pompei, P.; Ales, K.L.; MacKenzie, C.R. A new method of classifying prognostic comorbidity in longitudinal studies: Development and validation. J. Chronic Dis. 1987, 40, 373–383. [Google Scholar] [CrossRef] [PubMed]
- Sutherland, C.; Hare, D.; Johnson, P.J.; Linden, D.W.; Montgomery, R.A.; Droge, E. Practical advice on variable selection and reporting using Akaike information criterion. Proc. Biol. Sci. 2023, 290, 20231261. [Google Scholar] [CrossRef] [PubMed] [PubMed Central]
- Malick, A.; Wang, Y.; Axelrad, J.; Salmasian, H.; Freedberg, D. Obesity is not associated with adverse outcomes among hospitalized patients with Clostridioides difficile infection. Gut Pathog. 2022, 14, 7. [Google Scholar] [CrossRef] [PubMed]
- Nathanson, B.H.; Higgins, T.L.; McGee, W.T. The dangers of extreme body mass index values in patients with Clostridium difficile. Infection 2017, 45, 787–793. [Google Scholar] [CrossRef]
- Raseen, T.; Abdullah, A.; Abdul, W.; Mohammed, A.; Srishti, S.; Maryrose, L.-V.; Darrell, P.; Sahil, K. Outcomes of Clostridium difficile Infection in Patients with Obesity: A Nationwide Analysis: 208. Am. J. Gastroenterol. 2018, 113, S121–S122. [Google Scholar]
- Jaikumar, V.; Jha, S.; Naik, R.; Karki, A. Does Obesity Paradox Hold True for Clostridium Difficile Infection? A National Inpatient Database Analysis. In Proceedings of the Abstract Presentation (J8) at Society of Hospital Medicine (SHM) Converge 2022, Nashville, TN, USA, 7–10 April 2022. [Google Scholar]
- Schuijt, T.J.; Lankelma, J.M.; Scicluna, B.P.; de Sousa e Melo, F.; Roelofs, J.J.T.H.; de Boer, J.D.; Hoogendijk, A.J.; de Beer, R.; de Vos, A.; Belzer, C.; et al. The gut microbiota plays a protective role in the host defence against pneumococcal pneumonia. Gut 2016, 65, 575–583. [Google Scholar] [CrossRef]
- Lesniak, N.A.; Schubert, A.M.; Flynn, K.J.; Leslie, J.L.; Sinani, H.; Bergin, I.L.; Young, V.B.; Schloss, P.D. The Gut Bacterial Community Potentiates Clostridioides difficile Infection Severity. mBio 2022, 13, e0118322. [Google Scholar] [CrossRef]
- Hakim, H.; Dallas, R.; Wolf, J.; Tang, L.; Schultz-Cherry, S.; Darling, V.; Johnson, C.; Karlsson, E.A.; Chang, T.-C.; Jeha, S.; et al. Gut Microbiome Composition Predicts Infection Risk During Chemotherapy in Children with Acute Lymphoblastic Leukemia. Clin. Infect. Dis. 2018, 67, 541–548. [Google Scholar] [CrossRef] [PubMed]
- Todor, S.B.; Ichim, C. Microbiome Modulation in Pediatric Leukemia: Impact on Graft-Versus-Host Disease and Treatment Outcomes: A Narrative Review. Children 2025, 12, 166. [Google Scholar] [CrossRef]
Variable | Entire Cohort (n = 889) | BMI ≥ 30 kg/m2 (n = 131) | BMI < 30 kg/m2 (n = 758) | p-Value |
---|---|---|---|---|
Age, years, median (IQR) | 71 (59–83) | 65 (56–79) | 73 (60–84) | <0.01 |
BMI, kg/m2, median (IQR) | 24.4 (21.3–27.3) | 33.3 (31.2–37.1) | 23.6 (20.7–25.5) | <0.01 |
Female gender, n/total n. (%) | 460/888 (52%) | 69/131 (53%) | 391/757 (52%) | 0.8 |
CCI, median (IQR) | 5 (3–7) | 5 (3–7) | 5 (3–7) | 0.4 |
CCI ≥ 3 points, n (%) | 629 (71%) | 82 (63%) | 547 (72%) | 0.03 |
Assistance in ADL, n (%) | 395 (44%) | 51 (39%) | 344 (45%) | 0.2 |
Nursing home residency, n (%) | 138/887 (16%) | 18 (14%) | 120/756 (16%) | 0.5 |
Comorbidities: | ||||
Hypertension, n (%) | 400 (45%) | 62 (47%) | 338 (45%) | 0.6 |
Diabetes mellitus, n (%) | 237 (27%) | 57 (44%) | 180 (24%) | <0.01 |
Chronic kidney disease, n (%) | 115 (13%) | 17 (13%) | 98 (13%) | 0.9 |
Liver disease, n (%) | 21 (2%) | 6 (5%) | 15 (2%) | 0.07 |
Inflammatory bowel disease, n (%) | 31 (4%) | 1 (0.8%) | 30 (4%) | 0.07 |
Solid tumor, n (%) | 131 (15%) | 24 (18%) | 107 (14%) | 0.2 |
Hematologic malignancy, n (%) | 84 (9%) | 9 (7%) | 75 (10%) | 0.3 |
Solid organ transplant, n (%) | 62 (7%) | 8 (6%) | 54 (7%) | 0.7 |
HSC transplant, n (%) | 29 (3%) | 4 (3%) | 25 (3%) | 1 |
PPI treatment, n (%) | 369 (42%) | 59 (45%) | 310 (41%) | 0.4 |
Variable | Entire Cohort (n = 889) | BMI ≥ 30 kg/m2 (n = 131) | BMI < 30 kg/m2 (n = 758) | p-Value |
---|---|---|---|---|
Vital signs | ||||
Temperature, C°, median (IQR) | 36.9 (36.6–37.4) | 36.8 (36.6–37.3) | 36.9 (36.6–37.5) | 0.08 |
Systolic blood pressure, mmHg, median (IQR) | 119 (105–138) | 127 (112–141) | 118 (103–138) | <0.01 |
Laboratory results | ||||
WBC count, 109 cells/L, median (IQR) | 11.2 (6.72–17.3) | 9.39 (6.55–14.1) | 11.6 (6.8–17.4) | 0.01 |
Creatinine level, mg/dL, median (IQR) | 1.05 (0.73–1.65) | 1.04 (0.76–1.73) | 1.05 (0.72–1.64) | 0.5 |
Creatinine level > 1.5 mg/dL, n (%) | 248/864 (29%) | 39/129 (30%) | 209/735 (28%) | 0.7 |
Albumin, g/dL, mean ± SD | 3.18 ± 0.67 | 3.28 ± 0.71 | 3.16 ± 0.67 | 0.1 |
Albumin ≤ 3 g/dL, n/total n (%) | 316/718 (44%) | 41/105 (39%) | 275/613 (45%) | 0.3 |
CRP, mg/dL, median (IQR) | 6.9 (2.67–15.14) | 6.6 (2.86–13.7) | 7.2 (2.62–15.3) | 0.8 |
CRP ≥ 10 mg/dL, n/total n (%) | 245/639 (38%) | 34/96 (35%) | 211/543 (39%) | 0.5 |
Microbiological diagnosis by PCR, n (%) | 485 (55%) | 73 (56%) | 412 (54%) | 0.8 |
Severe CDI * | 590/814 (72%) | 80/119 (67%) | 510/695 (73%) | 0.2 |
CDI treatment | ||||
Vancomycin, n (%) | 554 (62%) | 87 (66%) | 467 (62%) | 0.3 |
Metronidazole, n (%) | 258 (29%) | 30 (23%) | 228 (30%) | 0.09 |
Fidaxomicin, n (%) | 80 (9%) | 14 (11%) | 66 (9%) | 0.5 |
Tigecyclin, n (%) | 27 (3%) | 3 (2%) | 24 (3%) | 0.8 |
Variable | BMI ≥ 30 kg/m2 (n = 131) | BMI < 30 kg/m2 (n = 758) | p-Value |
---|---|---|---|
90-day mortality, n/total n (%) | 19/131 (15%) | 170/752 (23%) | 0.04 |
30-day mortality, n/total n (%) | 8/131 (6%) | 96/757 (13%) | 0.03 |
ICU admission, n (%) | 9 (7%) | 23 (3%) | 0.03 |
Colectomy, n (%) | 2 (1.5%) | 6 (0.8%) | 0.3 |
Length of hospital stay, days, median (IQR) | 19 (7–33) | 12 (6–25) | <0.01 |
Variable | 90-Day Mortality | p-Value | |
---|---|---|---|
Yes (n = 189) | No (n = 694) | ||
Age, years, median (IQR) | 77 (65–86) | 70 (57–82) | <0.01 |
Female gender, n (%) | 95 (50%) | 362 (52%) | 0.6 |
BMI, kg/m2, median (IQR) | 23.4 (20.6–26) | 24.9 (21.6–28) | 0.008 |
Obesity (BMI ≥ 30 kg/m2) | 19 (10%) | 112 (16%) | 0.04 |
CCI, median (IQR) | 6 (4–8) | 5 (3–7) | <0.01 |
CCI ≥ 3 points, n (%) | 165 (87%) | 461 (66%) | <0.01 |
Assistance in ADL, n (%) | 105 (56%) | 288 (42%) | <0.01 |
Nursing home residency, n (%) | 31 (16%) | 106 (15%) | 0.7 |
Hypertension, n (%) | 94 (50%) | 305 (44%) | 0.2 |
Diabetes mellitus, n (%) | 54 (29%) | 182 (26%) | 0.5 |
Liver disease, n (%) | 5 (3%) | 16 (2%) | 0.8 |
Inflammatory bowel disease, n (%) | 0 (0%) | 29 (4%) | 0.002 |
Chronic kidney disease, n (%) | 28 (15%) | 86 (12%) | 0.4 |
Solid tumors, n (%) | 46 (24%) | 84 (12%) | <0.01 |
Hematologic malignancy, n (%) | 16 (9%) | 67 (10%) | 0.6 |
Solid organ transplant, n (%) | 6 (3%) | 56 (8%) | 0.02 |
HSCT, n (%) | 7 (4%) | 21 (3%) | 0.6 |
PPI treatment, n (%) | 93 (49%) | 274 (40%) | 0.02 |
WBC count, 109 cells/L, median (IQR) | 12.1 (6.8–19.6) | 10.9 (6.7–16.7) | 0.06 |
Creatinine level, mg/dL, median (IQR) | 1.11 (0.74–1.77) | 1.02 (0.73–1.6) | 0.3 |
Creatinine level > 1.5 mg/dL, n (%) | 60/187 (32%) | 188/677 (28%) | 0.2 |
Albumin, g/dL, mean ± SD | 2.98 ± 0.62 | 3.23 ± 0.68 | <0.01 |
Albumin ≤ 3 g/dL, n/total n (%) | 86/156 (55%) | 230/556 (41%) | <0.01 |
CRP, mg/dL, median (IQR) | 7.6 (3.54–0.85) | 6.6 (2.53–15.09) | 0.3 |
CRP > 10 mg/dL, n/total n (%) | 55 (40%) | 188 (38%) | 0.6 |
Microbiological diagnosis by PCR, n (%) | 93 (49%) | 387 (56%) | 0.1 |
Severe CDI *, n/total n (%) | 145/182 (80%) | 444/631 (70%) | 0.01 |
Colectomy, n (%) | 4 (2.1%) | 4 (0.6%) | 0.05 |
ICU admission, n (%) | 12 (6%) | 20 (3%) | 0.02 |
CDI treatment | |||
Metronidazole, n (%) | 59 (31%) | 199 (29%) | 0.5 |
Vancomycin, n (%) | 109 (58%) | 439 (63%) | 0.2 |
Fidaxomicin, n (%) | 19 (10%) | 60 (9%) | 0.5 |
Tigecyclin, n (%) | 7 (4%) | 20 (3%) | 0.6 |
Risk Factor | Adjusted OR (95% CI) | p-Value |
---|---|---|
Obesity (BMI ≥ 30 kg/m2) | 0.65 (0.38–1.01) | 0.1 |
Severe CDI * vs. non-severe | 1.47 (0.98–2.19) | 0.06 |
CCI ≥ 3 vs. <3 | 2.42 (1.40–4.18) | <0.01 |
PPI treatment | 1.35 (0.96–1.88) | 0.08 |
Age (years) | 1.01 (0.99–1.02) | 0.2 |
Assistance in ADL | 1.19 (0.83–1.72) | 0.3 |
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Atamna, A.; Khalaila, M.; Babich, T.; Zriek, A.; Ben Zvi, H.; Ayada, G.; Elis, A.; Bishara, J.; Nutman, A. The Impact of Obesity on Clostridioides difficile Infection Outcomes: A Retrospective Cohort Study. J. Clin. Med. 2025, 14, 5459. https://doi.org/10.3390/jcm14155459
Atamna A, Khalaila M, Babich T, Zriek A, Ben Zvi H, Ayada G, Elis A, Bishara J, Nutman A. The Impact of Obesity on Clostridioides difficile Infection Outcomes: A Retrospective Cohort Study. Journal of Clinical Medicine. 2025; 14(15):5459. https://doi.org/10.3390/jcm14155459
Chicago/Turabian StyleAtamna, Alaa, Manar Khalaila, Tanya Babich, Anan Zriek, Haim Ben Zvi, Gida Ayada, Avishay Elis, Jihad Bishara, and Amir Nutman. 2025. "The Impact of Obesity on Clostridioides difficile Infection Outcomes: A Retrospective Cohort Study" Journal of Clinical Medicine 14, no. 15: 5459. https://doi.org/10.3390/jcm14155459
APA StyleAtamna, A., Khalaila, M., Babich, T., Zriek, A., Ben Zvi, H., Ayada, G., Elis, A., Bishara, J., & Nutman, A. (2025). The Impact of Obesity on Clostridioides difficile Infection Outcomes: A Retrospective Cohort Study. Journal of Clinical Medicine, 14(15), 5459. https://doi.org/10.3390/jcm14155459