Exosomes Derived from Colon Cancer Cells Promote Tumor Progression and Affect the Tumor Microenvironment
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Exosome Isolation and Treatment
2.3. Immunoblotting
2.4. Transmission Electron Microscopy (TEM)
2.5. NanoSight Tracking Analysis (NTA)
2.6. MTT Assay
2.7. Wound-Healing Assay
2.8. Isolation and Culture of Cancer-Associated Fibroblasts (CAFs)
2.9. RNA Sequencing
2.10. Statistical Analysis
3. Results
3.1. Isolation of Colon Cancer-Cell-Derived Exosomes
3.2. Cellular Proliferation Induced by Colon Cancer-Cell-Derived Exosomes
3.3. EMT Induction by Colon Cancer-Cell-Derived Exosomes
3.4. RNA Sequencing Analysis of CAFs Treated with Colon Cancer Cell-Derived Exosomes
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, M.; Son, I.T.; Noh, G.T.; Woo, S.-Y.; Lee, R.-A.; Oh, B.Y. Exosomes Derived from Colon Cancer Cells Promote Tumor Progression and Affect the Tumor Microenvironment. J. Clin. Med. 2023, 12, 3905. https://doi.org/10.3390/jcm12123905
Kim M, Son IT, Noh GT, Woo S-Y, Lee R-A, Oh BY. Exosomes Derived from Colon Cancer Cells Promote Tumor Progression and Affect the Tumor Microenvironment. Journal of Clinical Medicine. 2023; 12(12):3905. https://doi.org/10.3390/jcm12123905
Chicago/Turabian StyleKim, Minsung, Il Tae Son, Gyoung Tae Noh, So-Youn Woo, Ryung-Ah Lee, and Bo Young Oh. 2023. "Exosomes Derived from Colon Cancer Cells Promote Tumor Progression and Affect the Tumor Microenvironment" Journal of Clinical Medicine 12, no. 12: 3905. https://doi.org/10.3390/jcm12123905