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Article

Long-Term Follow-Up of Fractional CO2 Laser Therapy for Genitourinary Syndrome of Menopause in Breast Cancer Survivors

by
Allison M. Quick
1,*,
Andrew Hundley
2,
Cynthia Evans
2,
Julie A. Stephens
3,
Bhuvaneswari Ramaswamy
4,
Raquel E. Reinbolt
4,
Anne M. Noonan
4,
Jeffrey Bryan Van Deusen
4,
Robert Wesolowski
4,
Daniel G. Stover
4,
Nicole Olivia Williams
4,
Sagar D. Sardesai
4,
Stephanie S. Faubion
5,
Charles L. Loprinzi
6 and
Maryam B. Lustberg
7
1
Department of Radiation Oncology, The Ohio State University Medical Center, Columbus, OH 43210, USA
2
Department of Obstetrics and Gynecology, The Ohio State University Medical Center, Columbus, OH 43210, USA
3
The Ohio State University Center for Biostatistics, Columbus, OH 43210, USA
4
Division of Medical Oncology, The Ohio State University Medical Center, Columbus, OH 43210, USA
5
Department of Medicine, Mayo Clinic, Jacksonville, FL 32256, USA
6
Division of Medical Oncology, Mayo Clinic, Rochester, MN 55902, USA
7
Division of Medical Oncology Yale Cancer Center, New Haven, CT 06520, USA
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2022, 11(3), 774; https://doi.org/10.3390/jcm11030774
Submission received: 11 January 2022 / Revised: 25 January 2022 / Accepted: 27 January 2022 / Published: 31 January 2022
(This article belongs to the Special Issue Side Effects of Cancer Therapeutics in Clinical Practice)
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Abstract

:
(1) Background: The objective of this study was to determine the long-term efficacy of fractional CO2 laser therapy in breast cancer survivors. (2) Methods: This was a single-arm study of breast cancer survivors. Participants received three treatments of fractional CO2 laser therapy and returned for a 4 week follow-up. Participants were contacted for follow-up at annual intervals. The Vaginal Assessment Scale (VAS), the Female Sexual Function Index (FSFI), the Female Sexual Distress Scare Revised (FSDS-R), the Urinary Distress Inventory (UDI), and adverse events were collected and reported for the two-year follow-up. The changes in scores were compared between the four-week and two-year and the one-year and two-year follow-ups using paired t-tests. (3) Results: In total, 67 BC survivors were enrolled, 59 completed treatments and the four week follow-up, 39 participated in the one-year follow-up, and 33 participated in the two-year follow-up. After initial improvement in the VAS from baseline to the four week follow-up, there was no statistically significant difference in the VAS score (mean Δ 0.23; 95% CI [−0.05, 0.51], p = 0.150) between the four week follow-up and the two-year follow-up. At the two-year follow-up, the FSFI and FSDS-R scores remained improved from baseline and there was no statistically significant change in the FSFI score (mean Δ −0.83; 95% CI [−3.07, 2.38] p = 0.794) or the FSDS-R score (mean Δ −2.85; 95% CI [−1.88, 7.59] p = 0.227) from the one to two-year follow-up. The UDI scores approached baseline at the two-year follow-up; however, the change between the one- and two-year follow-ups was not statistically significant (mean Δ 4.76; 95% CI [−1.89, 11.41], p = 0.15). (4) Conclusions: Breast cancer survivors treated with fractional CO2 laser therapy have sustained improvement in sexual function two years after treatment completion, suggesting potential long-term benefit.

1. Introduction

Genitourinary syndrome of menopause (GSM) is a constellation of symptoms that affect many breast cancer survivors due to the direct effect of cancer treatments, from change in menopausal status related to treatment, or from discontinuation of the use of hormone therapy because of their diagnosis [1,2,3]. Symptoms of GSM include vaginal dryness and dyspareunia that can affect long-term quality of life [1,4,5]. The vaginal changes from estrogen deprivation after menopause can lead to chronic symptoms that worsen over time without treatment [3,6]. While low-dose vaginal estrogens are a treatment approach for postmenopausal women, concerns about systemic absorption limit their use in the breast cancer population [6].
Fractional CO2 laser therapy is a new modality that has been used to treat GSM in postmenopausal women [7,8,9,10,11,12], with results similar to those observed with vaginal estrogen treatment [13,14,15,16]. Fractional CO2 laser therapy remodels vaginal tissue by activation of fibroblasts and increasing collagen production and neovascularization [17] through direct controlled thermal effect on the vaginal mucosa [18]. Two recent small, randomized sham-controlled studies showed improvement in GSM symptoms with laser treatment compared to sham treatment [19,20]. Fractional CO2 laser treatment has also been used in women with cancer, most commonly breast cancer. Data based on retrospective [21,22,23] and several small single-arm studies have shown improvement in vaginal symptoms and sexual function [24,25,26,27,28]. A major limitation of these studies has been a lack of long-term follow-up to evaluate persistence of benefit of this intervention.
We conducted a pilot study of fractional CO2 laser treatment in breast cancer survivors with GSM and demonstrated feasibility based on 88% of those enrolled completing all treatments according to protocol without serious adverse events. In addition, the treatment appeared to reduce symptoms of GSM at the initial one-month follow-up [25]. This study protocol was amended to collect follow-up data at one and two years to augment the limited long-term data on this treatment. Patient-reported sexual function was collected at one-year and demonstrated improvement in the FSFI and FSDS-R scores [26]. Here, we report the two-year follow-up results.

2. Materials and Methods

2.1. Sample and Eligibility

Women with non-metastatic breast cancer with symptoms of GSM including dyspareunia and/or vaginal dryness were recruited for this study as previously reported [25]. Patients had completed surgery, chemotherapy, and/or radiation for their breast cancer. They may have been on endocrine therapy and/or trastuzumab at the time of this study.
Exclusion criteria for this study were metastatic breast cancer, vaginal stenosis preventing placement of the vaginal probe, active urogenital infection, stage II or higher pelvic organ prolapse, a history of mesh-based reconstructive pelvic surgery, and systemic estrogen therapy or vaginal estrogen or dehydroepiandrosterone within six weeks prior to enrollment.
Women meeting the eligibility criteria and willing to participate consented for treatment. This study and the amendment were approved by The Ohio State University Cancer Institutional Review Board in July 2017 and May 2019, respectively. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

2.2. Procedures

Demographics, clinical data, and inclusion/exclusion criteria were obtained for all participants and have been previously reported [25]. All women were contacted for two-year follow-up either by phone or during a routine clinic visit to medical oncology or urogynecology. Three attempts were made by phone for follow-up assessment before classifying the participant as lost to follow-up. Subjective and objective symptoms of GSM were assessed at baseline and the 4 week follow-up. Subjective symptoms were also assessed at the two-year follow-up. Additional questionnaires assessing sexual function and urinary function were assessed at the same time points and were also collected at one-year follow-up. Adverse events (AE) were also solicited.
The treatment protocol which consisted of fractional microablative CO2 laser (MonaLisa TouchTM, DEKA Florence, Italy) at three time points 30–45 days apart has been previously described in detail [25]. The vaginal probe was inserted and laser pulses were delivered to the entire circumference and length of the vagina. The vestibule and posterior fourchette were treated using an external probe. For 48 h prior to and following the procedure, participants were informed to abstain from sexual intercourse and the use of topical vaginal products.

2.3. Measures

Subjective vaginal symptoms: The Vaginal and Vulvar Assessment Scales (VAS and VuAS), validated clinical measurement tools where a lower score indicated better function, were used to assess vaginal symptoms and dyspareunia over the preceding 4 weeks [29,30]. Because the VAS was designed as a physician-reported measure, it was initially not collected at the one-year follow-up since many participants were contacted via phone. With permission of the developer of the VAS [30], we used a patient-reported version of the questionnaire with the same questions and collected this information at the two-year follow-up.
Sexual function and distress: Sexual function was measured using the Female Sexual Function Index (FSFI), a 19 item self-reported instrument that measures six domains of sexual functioning and has been validated in breast cancer survivors [31]. A score of ≤26.55 indicates risk for sexual dysfunction, with higher scores indicating better sexual function [32]. The Female Sexual Distress Scale-Revised (FSDS-R) is a 13-item self-reported instrument designed to measure sexually-related psychological distress in women [33]. A higher score indicates more sexual distress and a total score of ≥11 shows high sensitivity (93%), specificity (96%), and positive predictive value (87.5%) for low libido after menopause [33,34].
Urinary function: Urinary symptoms were assessed using the Urogenital Distress Inventory-6 (UDI-6), a validated 6 item questionnaire with three subscales with scores that are added for a total score, with a higher score indicating higher disability [35]. The average score was calculated for the answered items only. The average UDI was multiplied by 25 to scale the total to a score of 100. If a subject answered “No” for the first question and “Not at All” for the second question, they were given a score of zero.
Adverse Events: AE were self-reported prior to each treatment and at the 4 week, one-year and two-year follow-ups. They were graded according to the NCI Common Terminology Criteria for Adverse Events v4.0 (CTCAE).
Statistical analysis: Estimates were calculated on the subset of participants who agreed to participate in the two-year follow-up. Descriptive statistics including means and 95% confidence intervals for continuous variables and proportions for categorical variables were used to summarize demographics, clinical characteristics and endpoints. The changes in endpoints from the four-week and one-year to the two-year follow-up were compared using paired t-tests. Although we present p-values in this paper, we consider these to be descriptive and hypothesis generating. All analyses were performed using SAS Version 9.4 (SAS Inc., Cary, NC, USA). All statistical tests were two sided with a 0.05 level of significance.

3. Results

A total of 67 patients enrolled in the initial pilot study and 3 withdrew from this study at screening, resulting in a total of 64 treated participants. Five patients withdrew after starting treatment. After extending follow-up of the study participants, 41 of the initial 59 women agreed to participate in the one-year follow-up and 33 were able to be contacted and agreed to participate in the two-year follow-up (Figure 1). Participant and breast cancer treatment characteristics are summarized in Table 1.

3.1. VAS/VuAS

We previously demonstrated that there was a statistically significant decrease in the total VAS score from baseline to the four-week follow-up, indicating an improvement in vaginal symptoms [25]. With long-term follow-up, there was a slight increase in the mean VAS score (n = 31) from the four-week follow-up to the two-year follow-up but it was not statistically significant (mean Δ 0.23; 95% CI [−0.05, 0.51], p = 0.150).
Similarly, there was initially a statistically significant improvement in the VuAS score from baseline to the four-week follow-up [25]. With additional long-term follow-up, there was no statistically significant difference between the four-week follow-up and the two-year follow-up scores (mean Δ −0.03; 95% CI [−0.33, 0.26] p = 0.817). Figure 2 shows VAS and VuAS scores at baseline, four-week follow-up, and two-year follow-up.

3.2. Sexual Function

At the two-year follow-up, the sexual function questionnaire scores remained improved from baseline and were stable from the one-year follow-up. There was no statistically significant change in the FSFI score (mean Δ −0.83; 95% CI [−3.07, 2.38] p = 0.794) or the FSDS-R scores (mean Δ −2.85; 95% CI [−1.88, 7.59] p = 0.227) from the one to the two-year follow-up. In fact, approximately 91% of participants had a FSDS-R score of ≥11 at baseline, indicating sexually-related distress, compared to 54% at the one-year follow-up and 52% at the two-year follow-up. Figure 3 shows the FSFI and FSDS scores at baseline as well as the four-week, one-year, and two-year follow-ups.

3.3. Urinary Symptoms

While UDI scores decreased, suggesting improvement, at the initial four-week follow-up and one-year follow-up, they approached baseline at the two-year follow-up. However, the change between the one- and two-year follow-ups was not statistically significant (mean Δ 4.76; 95% CI [−1.89, 11.41], p = 0.155). Figure 4 shows the UDI scores at baseline as well as the four-week, one-year, and two-year follow-ups.

3.4. Adverse Events

No grade 3 or higher adverse events were identified at the two-year follow-up.

4. Discussion

This study found that initial improvement in sexual function noted after fractional CO2 laser therapy in breast cancer survivors was sustained at two years post-treatment, in a subset of the initial study population. In contrast, the initial improvement in vaginal and urinary symptoms approached baseline levels after two years. This suggests the potential return of some symptoms over time without additional treatments. There was no SAE reported with long-term follow-up.
Approximately 50% of postmenopausal women will suffer symptoms of GSM that can be unrelenting without treatment [3]. Although vaginal dryness is typically the most bothersome symptom, it is often associated with sexual dysfunction [3]. The number of breast cancer survivors with GSM is higher than the general population of postmenopausal women, particularly breast cancer survivors on aromatase inhibitors (AI) [1,5,36,37]. Due to the severity of symptoms, some women discontinue AI therapy, potentially compromising their cancer outcomes [38]. Topical lubricants and moisturizers can be used for mild symptoms but symptoms often progress despite their use [3]. Low-dose vaginal estrogen use continues to be controversial in breast cancer survivors [39] but may have a role in some women with breast cancer with severe symptoms after close consultation with the oncologist for discussion of risk with treatment [40,41]. While permitted as second line therapy for dyspareunia by ESO-ESMO international consensus guidelines [42], topical hormone therapy did not improve vaginal symptoms or sexual problems in women with breast cancer in a recent retrospective study [43].
Laser treatments, including fractional CO2 laser therapy [11,12,15,18,44,45], erbium laser therapy (Er:YAG) [46,47], and other energy based devices, such as radiofrequency treatments [48], are being used to treatment symptoms of GSM. A meta-analysis of vaginal laser treatments [CO2 laser (n = 10 papers) and Er: YAG (n = 4 papers)] concluded that laser therapy appears to reduce symptoms of GSM and improve quality of life [44]. Similar to vaginal estrogen treatment, vaginal laser treatment has been shown to increase the thickness of vaginal epithelium and improve the quality of the vaginal mucosa [14]. However, there is still limited prospective data with long-term follow-up and there are some studies which failed to show an improvement with laser treatment [49,50].
The results of the current study are consistent with other prospective single-arm studies of fractional CO2 laser therapy in postmenopausal women and women with breast cancer. There are very few studies with long-term follow-up, however, limiting knowledge about duration of treatment effects and long-term side effects. A prospective single-arm study by Veron et al. demonstrated improvement in sexual and urinary function with laser treatment in breast cancer survivors (n = 46). The improvement in sexual function persisted at longer-term follow-up of 18 months. There was no patient-reported vaginal dryness or other vaginal symptoms reported [28]. The current study is the first to report follow-up at two years and supports sustained benefit in sexual function but suggests the possibility that the vaginal and urinary symptom benefit may be of shorter duration and may require additional treatments beyond one year.
Sham-controlled studies have mixed results. A small randomized double-blind, sham-controlled study in 30 postmenopausal women did not demonstrate a difference in vaginal symptoms, sexual function, or urinary symptoms between the treatment arm (n = 14) and the sham-controlled arm (n = 16). However, the study was likely underpowered due to its small sample size [49]. Another recently published sham-controlled randomized trial of 85 postmenopausal women, 50% of whom had a history of breast cancer, also showed no improvement in vaginal symptoms with laser treatment compared to sham treatment with 12 months follow-up [50]. However, a randomized double-blind, sham-controlled study of 58 postmenopausal women by Salvatore et al. showed that vaginal symptoms and FSFI and UDI-6 scores were improved in the treatment group compared to the sham group [19]. Another randomized sham-controlled pilot study was also conducted in women with gynecologic cancers which showed that laser treatments were safe and appeared to improve sexual function based on FSFI scores [51].
While the majority of studies have used three laser treatments, the exact number of treatments needed to achieve and maintain benefit remains unknown. A recent small prospective pilot study of 40 women with breast cancer delivered five laser treatments every four weeks and were re-evaluated at a four-week follow-up. The majority of women were satisfied with treatment and experienced improvement in subjective and objective symptoms of vulvovaginal atrophy, quality of life, and sexual function [52].
The strength of the current study is that it is has the longest follow-up of any the reported study on fractional CO2 laser therapy, including postmenopausal women or cancer survivors, which continues to support the lack of long-term adverse event from laser treatment.
The limitations of the study include lack of a sham comparison arm and the loss of a significant number of the treated women resulting in bias in the scores of the subjects who agreed to participate through two-year follow-up, compared to those who did not. Additionally, this study was not powered to detect differences at the two-year follow-up. Therefore, a double-blind, randomized controlled trial, comparing fractional CO2 laser therapy versus sham laser treatment is needed to further establish the short- and long-term safety and efficacy of CO2 laser treatment in women with breast cancer.

5. Conclusions

Breast cancer survivors treated with fractional CO2 laser therapy reported sustained improvement in sexual function with no serious adverse effects two years after initial treatment in this limited study.

Author Contributions

Conceptualization, A.M.Q., C.L.L., M.B.L., B.R., A.H. and C.E.; methodology, A.M.Q., C.L.L., M.B.L., A.H. and C.E.; software, J.A.S.; formal analysis, J.A.S.; investigation, A.M.Q., A.H., C.E., J.A.S., B.R., R.E.R., A.M.N., J.B.V.D., R.W., D.G.S., N.O.W., S.D.S., S.S.F., C.L.L. and M.B.L.; resources, A.H. and C.E.; data curation, J.A.S.; writing—original draft preparation, A.M.Q.; writing—review and editing, A.H., C.E., J.A.S., B.R., R.E.R., A.M.N., J.B.V.D., R.W., D.G.S., N.O.W., S.D.S., S.S.F., C.L.L. and M.B.L.; visualization, A.M.Q. and J.A.S.; supervision, C.L.L. and M.B.L.; project administration, A.M.Q. and M.B.L.; funding acquisition, A.M.Q. and M.B.L. All authors have read and agreed to the published version of the manuscript.

Funding

Funding was provided by OSU FAME PRO (AQ) and by the OSU Spielman Development Funds (ML).

Institutional Review Board Statement

This study was conducted according to the guidelines of the Declaration of Helsinki, and approved by the Institutional Review Board (or Ethics Committee) of The Ohio State University Medical Center (OSU-17153 and 9 July 2017 and amendment 9 May 2019).

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study.

Data Availability Statement

The corresponding author has full control of the primary data and can be reviewed by the journal if requested.

Acknowledgments

Acknowledgement is given to Jeanne Carter, for permitting the use of the patient reported version of the VAS/VuAS for this study.

Conflicts of Interest

The authors declare no conflict of interest. No financial support was provided by Hologic, Inc. The laser devise was provided by loan to OSU from Hologic/Cynosure. None of the investigators received financial support from Hologic/Cynosure. The company was not involved in study data analysis or manuscript preparation.

References

  1. Falk, S.J.; Bober, S. Vaginal health during breast cancer treatment. Curr. Oncol. Rep. 2016, 18, 32. [Google Scholar] [CrossRef] [PubMed]
  2. Faubion, S.S.; Sood, R.; Kapoor, E. Genitourinary syndrome of menopause: Management strategies for the clinician. Mayo Clin. Proc. 2017, 92, 1842–1849. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  3. Phillips, N.A.; Bachmann, G.A. The genitourinary syndrome of menopause. Menopause 2021, 28, 579–588. [Google Scholar] [CrossRef] [PubMed]
  4. Melisko, M.E.; Goldman, M.E.; Hwang, J.; De Luca, A.; Fang, S.; Esserman, L.J.; Chien, A.J.; Park, J.W.; Rugo, H.S. Vaginal testosterone cream vs estradiol vaginal ring for vaginal dryness or decreased libido in women receiving aromatase inhibitors for early-stage breast cancer. JAMA Oncol. 2017, 3, 313–319. [Google Scholar] [CrossRef]
  5. Schover, L.R.; Baum, G.P.; Fuson, L.A.; Brewster, A.; Melhem-Bertrandt, A. sexual problems during the first 2 years of adjuvant treatment with aromatase inhibitors. J. Sex. Med. 2014, 11, 3102–3111. [Google Scholar] [CrossRef] [Green Version]
  6. Faubion, S.S.; Larkin, L.C.; Stuenkel, C.A.; Bachmann, G.A.; Chism, L.A.; Kagan, R.; Kaunitz, A.M.; Krychman, M.L.; Parish, S.J.; Partridge, A.H.; et al. Management of genitourinary syndrome of menopause in women with or at high risk for breast cancer: Consensus recommendations from The North American Menopause Society and The International Society for the Study of Women’s Sexual Health. Menopause 2018, 25, 596–608. [Google Scholar] [CrossRef]
  7. Perino, A.; Calligaro, A.; Forlani, F.; Tiberio, C.; Cucinella, G.; Svelato, A.; Saitta, S.; Calagna, G. Vulvo-vaginal atrophy: A new treatment modality using thermo-ablative fractional CO2 laser. Maturitas 2015, 80, 296–301. [Google Scholar] [CrossRef]
  8. Sokol, E.R.; Karram, M.M. An assessment of the safety and efficacy of a fractional CO2 laser system for the treatment of vulvovaginal atrophy. Menopause 2016, 23, 1102–1107. [Google Scholar] [CrossRef]
  9. Pitsouni, E.; Grigoriadis, T.; Tsiveleka, A.; Zacharakis, D.; Salvatore, S.; Athanasiou, S. Microablative fractional CO2 -laser therapy and the genitourinary syndrome of menopause: An observational study. Maturitas 2016, 94, 131–136. [Google Scholar] [CrossRef]
  10. Salvatore, S.; Nappi, R.E.; Zerbinati, N.; Calligaro, A.; Ferrero, S.; Origoni, M.; Candiani, M.; Maggiore, U.L.R. A 12-week treatment with fractional CO2 laser for vulvovaginal atrophy: A pilot study. Climacteric 2014, 17, 363–369. [Google Scholar] [CrossRef]
  11. Athanasiou, S.; Pitsouni, E.; Grigoriadis, T.; Zacharakis, D.; Falagas, M.E.; Salvatore, S.; Protopapas, A.; Loutradis, D. Microablative fractional CO2 laser for the genitourinary syndrome of menopause: Up to 12-month results. Menopause 2019, 26, 248–255. [Google Scholar] [CrossRef] [PubMed]
  12. Behnia-Willison, F.; Sarraf, S.; Miller, J.; Mohamadi, B.; Care, A.; Lam, A.; Willison, N.; Behnia, L.; Salvatore, S. Safety and long-term efficacy of fractional CO2 laser treatment in women suffering from genitourinary syndrome of menopause. Eur. J. Obstet. Gynecol. Reprod. Biol. 2017, 213, 39–44. [Google Scholar] [CrossRef] [PubMed]
  13. Paraiso, M.F.R.; Ferrando, C.A.; Sokol, E.R.; Rardin, C.R.; Matthews, C.A.; Karram, M.M.; Iglesia, C.B. A randomized clinical trial comparing vaginal laser therapy to vaginal estrogen therapy in women with genitourinary syndrome of menopause: The VeLVET Trial. Menopause 2020, 27, 50–56. [Google Scholar] [CrossRef] [PubMed]
  14. Dutra, P.F.S.P.; Heinke, T.; Pinho, S.C.; Focchi, G.R.A.; Tso, F.K.; de Almeida, B.C.; Silva, I.; Speck, N.M.G. Comparison of topical fractional CO2 laser and vaginal estrogen for the treatment of genitourinary syndrome in postmenopausal women. Menopause 2021, 28, 756–763. [Google Scholar] [CrossRef]
  15. Cruz, V.L.; Steiner, M.L.; Pompei, L.M.; Strufaldi, R.; Fonseca, F.L.A.; Santiago, L.H.S.; Wajsfeld, T.; Fernandes, C.E. Randomized, double-blind, placebo-controlled clinical trial for evaluating the efficacy of fractional CO2 laser compared with topical estriol in the treatment of vaginal atrophy in postmenopausal women. Menopause 2018, 25, 21–28. [Google Scholar] [CrossRef]
  16. Li, J.; Li, H.; Zhou, Y.; Xie, M.; Miao, Y.; Wang, L.; Zhao, Y.; Ying, T.; Hu, Y.; Chen, Y.; et al. The Fractional CO2 laser for the treatment of genitourinary syndrome of menopause: A prospective multicenter cohort study. Lasers Surg. Med. 2020, 53, 647–653. [Google Scholar] [CrossRef]
  17. Stefano, S.; Stavros, A.; Massimo, C. The use of pulsed CO2 lasers for the treatment of vulvovaginal atrophy. Curr. Opin. Obstet. Gynecol. 2015, 27, 504–508. [Google Scholar] [CrossRef]
  18. Sokol, E.R.; Karram, M.M. Use of a novel fractional CO2 laser for the treatment of genitourinary syndrome of menopause: 1-year outcomes. Menopause 2017, 24, 810–814. [Google Scholar] [CrossRef]
  19. Salvatore, S.; Pitsouni, E.; Grigoriadis, T.; Zacharakis, D.; Pantaleo, G.; Candiani, M.; Athanasiou, S. CO2 laser and the genitourinary syndrome of menopause: A randomized sham-controlled trial. Climacteric J. Internat. Menopause Soc. 2021, 24, 187–193. [Google Scholar] [CrossRef]
  20. Ruanphoo, P.; Bunyavejchevin, S. Treatment for vaginal atrophy using microablative fractional CO2 laser: A randomized double-blinded sham-controlled trial. Menopause 2020, 27, 858–863. [Google Scholar] [CrossRef]
  21. Gittens, P.; Mullen, G. The effects of fractional microablative CO2 laser therapy on sexual function in postmenopausal women and women with a history of breast cancer treated with endocrine therapy. J. Cosmet. Laser Ther. 2019, 21, 127–131. [Google Scholar] [CrossRef] [PubMed]
  22. Pagano, T.; De Rosa, P.; Vallone, R.; Schettini, F.; Arpino, G.; De Placido, S.; Nazzaro, G.; Locci, M.; De Placido, G. Fractional microablative CO2 laser for vulvovaginal atrophy in women treated with chemotherapy and/or hormonal therapy for breast cancer: A retrospective study. Menopause 2016, 23, 1108–1113. [Google Scholar] [CrossRef] [PubMed]
  23. Pagano, T.; De Rosa, P.; Vallone, R.; Schettini, F.; Arpino, G.; Giuliano, M.; Lauria, R.; De Santo, I.; Conforti, A.; Gallo, A.; et al. Fractional microablative CO2 laser in breast cancer survivors affected by iatrogenic vulvovaginal atrophy after failure of nonestrogenic local treatments: A retrospective study. Menopause 2017, 25, 657–662. [Google Scholar] [CrossRef] [PubMed]
  24. Pearson, A.; Booker, A.; Tio, M.; Marx, G. Vaginal CO2 laser for the treatment of vulvovaginal atrophy in women with breast cancer: LAAVA pilot study. Breast Cancer Res. Treat. 2019, 178, 135–140. [Google Scholar] [CrossRef] [PubMed]
  25. Quick, A.M.; Zvinovski, F.; Hudson, C.; Hundley, A.; Evans, C.; Suresh, A.; Stephens, J.A.; Arthur, E.; Ramaswamy, B.; Reinbolt, R.E.; et al. Fractional CO2 laser therapy for genitourinary syndrome of menopause for breast cancer survivors. Support. Care Cancer 2020, 28, 3669–3677. [Google Scholar] [CrossRef] [PubMed]
  26. Quick, A.M.; Zvinovski, F.; Hudson, C.; Hundley, A.; Evans, C.; Stephens, J.A.; Arthur, E.; Ramaswamy, B.; Reinbolt, R.E.; Noonan, A.M.; et al. Patient-reported sexual function of breast cancer survivors with genitourinary syndrome of menopause after fractional CO2 laser therapy. Menopause 2021, 28, 642–649. [Google Scholar] [CrossRef]
  27. Pieralli, A.; Fallani, M.G.; Becorpi, A.; Bianchi, C.; Corioni, S.; Longinotti, M.; Tredici, Z.; Guaschino, S. Fractional CO2 laser for vulvovaginal atrophy (VVA) dyspareunia relief in breast cancer survivors. Arch. Gynecol. Obstet. 2016, 294, 841–846. [Google Scholar] [CrossRef]
  28. Veron, L.; Wehrer, D.; Annerose-Zéphir, G.; Suciu, V.; Delaloge, S.; Pistilli, B.; Chaltiel, D.; Pautier, P. Effects of local laser treatment on vulvovaginal atrophy among women with breast cancer: A prospective study with long-term follow-up. Breast Cancer Res. Treat. 2021, 1–9. [Google Scholar] [CrossRef]
  29. Carter, J.; Stabile, C.; Seidel, B.; Baser, R.E.; Goldfarb, S.; Goldfrank, D.J. Vaginal and sexual health treatment strategies within a female sexual medicine program for cancer patients and survivors. J. Cancer Surviv. Res. Pract. 2017, 11, 274–283. [Google Scholar] [CrossRef] [Green Version]
  30. Eaton, A.A.; Baser, R.E.; Seidel, B.; Stabile, C.; Canty, J.P.; Goldfrank, D.J.; Carter, J. Validation of clinical tools for vaginal and vulvar symptom assessment in cancer patients and survivors. J. Sex. Med. 2017, 14, 144–151. [Google Scholar] [CrossRef] [Green Version]
  31. Bartula, I.; Sherman, K.A. The female sexual functioning index (FSFI): Evaluation of acceptability, reliability, and validity in women with breast cancer. Support. Care Cancer 2015, 23, 2633–2641. [Google Scholar] [CrossRef] [PubMed]
  32. Rosen, R.; Brown, C.; Heiman, J.; Leiblum, S.; Meston, C.; Shabsigh, R.; Ferguson, D.; D’Agostino, R., Jr. The female sexual function index (FSFI): A multidimensional self-report instrument for the assessment of female sexual function. J. Sex. Marital Ther. 2000, 26, 191–208. [Google Scholar] [CrossRef] [PubMed]
  33. DeRogatis, L.; Clayton, A.; Lewis-D’Agostino, D.; Wunderlich, G.; Fu, Y. Validation of the female sexual distress scale-revised for assessing distress in women with hypoactive sexual desire disorder. J. Sex. Med. 2008, 5, 357–364. [Google Scholar] [CrossRef] [PubMed]
  34. Derogatis, L.R.; Rosen, R.; Leiblum, S.; Burnett, A.; Heiman, J. The female sexual distress scale (FSDS): Initial validation of a standardized scale for assessment of sexually related personal distress in women. J. Sex Marital Ther. 2002, 28, 317–330. [Google Scholar] [CrossRef]
  35. Uebersax, J.S.; Wyman, J.F.; Shumaker, S.A.; McClish, D.K.; Fantl, J.A. Short forms to assess life quality and symptom distress for urinary incontinence in women: The incontinence impact questionnaire and the urogenital distress inventory. Continence Program for Women Research Group. Neurourol. Urodyn. 1995, 14, 131–139. [Google Scholar] [CrossRef]
  36. Raggio, G.A.; Butryn, M.L.; Arigo, D.; Mikorski, R.; Palmer, S.C. Prevalence and correlates of sexual morbidity in long-term breast cancer survivors. Psychol. Health 2014, 29, 632–650. [Google Scholar] [CrossRef]
  37. Oberguggenberger, A.; Martini, C.; Huber, N.; Fallowfield, L.; Hubalek, M.; Daniaux, M.; Sperner-Unterweger, B.; Holzner, B.; Sztankay, M.; Gamper, E.; et al. Self-reported sexual health: Breast cancer survivors compared to women from the general population—An observational study. BMC Cancer 2017, 17, 599. [Google Scholar] [CrossRef]
  38. Hershman, D.L.; Shao, T.; Kushi, L.H.; Buono, D.; Tsai, W.Y.; Fehrenbacher, L.; Kwan, M.; Gomez, S.L.; Neugut, A.I. Early discontinuation and non-adherence to adjuvant hormonal therapy are associated with increased mortality in women with breast cancer. Breast Cancer Res. Treat. 2011, 126, 529–537. [Google Scholar] [CrossRef] [Green Version]
  39. Farrell, R. ACOG Committee Opinion No. 659: The use of vaginal estrogen in women with a history of estrogen-dependent breast cancer. Obstet. Gynecol. 2016, 127, e93–e96. [Google Scholar] [CrossRef]
  40. Hirschberg, A.L.; Bitzer, J.; Cano, A.; Ceausu, I.; Chedraui, P.; Durmusoglu, F.; Erkkola, R.; Goulis, D.G.; Kiesel, L.; Lopes, P.; et al. Topical estrogens and non-hormonal preparations for postmenopausal vulvovaginal atrophy: An EMAS clinical guide. Maturitas 2021, 148, 55–61. [Google Scholar] [CrossRef]
  41. NAMS Position Statement. The 2020 genitourinary syndrome of menopause position statement of The North American Menopause Society. Menopause 2020, 27, 976–992. [Google Scholar] [CrossRef] [PubMed]
  42. Cardoso, F.; Paluch-Shimon, S.; Senkus, E.; Curigliano, G.; Aapro, M.; André, F.; Barrios, C.; Bergh, J.; Bhattacharyya, G.; Biganzoli, L.; et al. 5th ESO-ESMO international consensus guidelines for advanced breast cancer (ABC 5). Ann. Oncol. 2020, 31, 1623–1649. [Google Scholar] [CrossRef] [PubMed]
  43. Jain, A.L.; Jamy, O.; Mullins, J.; Usman, R.M.; Hare, F.; Valasareddy, P.; Chaudhry, A.; Ryder, J.; Smith, J.R.; Miller, E.; et al. Usefulness of patient-reported outcomes to assess the effectiveness of topical hormonal therapy for gynecologic symptoms symptoms after antihormonal treatment for breast cancer. Bayl. Univ. Med Cent. Proc. 2020, 33, 331–335. [Google Scholar] [CrossRef] [PubMed]
  44. Pitsouni, E.; Grigoriadis, T.; Falagas, M.E.; Salvatore, S.; Athanasiou, S. Laser therapy for the genitourinary syndrome of menopause. A systematic review and meta-analysis. Maturitas 2017, 103, 78–88. [Google Scholar] [CrossRef] [PubMed]
  45. Filippini, M.; Luvero, D.; Salvatore, S.; Pieralli, A.; Montera, R.; Plotti, F.; Candiani, M.; Angioli, R. Efficacy of fractional CO2 laser treatment in postmenopausal women with genitourinary syndrome: A multicenter study. Menopause 2020, 27, 43–49. [Google Scholar] [CrossRef] [PubMed]
  46. Gambacciani, M.; Levancini, M. Vaginal erbium laser as second-generation thermotherapy for the genitourinary syndrome of menopause: A pilot study in breast cancer survivors. Menopause 2017, 24, 316–319. [Google Scholar] [CrossRef] [PubMed]
  47. Mothes, A.R.; Runnebaum, M.; Runnebaum, I.B. Ablative dual-phase Erbium:YAG laser treatment of atrophy-related vaginal symptoms in post-menopausal breast cancer survivors omitting hormonal treatment. J. Cancer Res. Clin. Oncol. 2018, 144, 955–960. [Google Scholar] [CrossRef]
  48. Vicariotto, F.; Raichi, M. Technological evolution in the radiofrequency treatment of vaginal laxity and menopausal vulvo-vaginal atrophy and other genitourinary symptoms: First experiences with a novel dynamic quadripolar device. Minerva Ginecol. 2016, 68, 225–236. [Google Scholar]
  49. Cruff, J.; Khandwala, S. A double-blind randomized sham-controlled trial to evaluate the efficacy of fractional carbon dioxide laser therapy on genitourinary syndrome of menopause. J. Sex. Med. 2021, 18, 761–769. [Google Scholar] [CrossRef]
  50. Li, F.; Picard-Fortin, V.; Maheux-Lacroix, S.; Deans, R.; Nesbitt-Hawes, E.; McCormack, L.; Abbott, P.J. The efficacy of vaginal laser and other energy-based treatments on genital symptoms in postmenopausal women: A systematic review and meta-analysis. J. Minim. Invasive Gynecol. 2021, 28, 668–683. [Google Scholar] [CrossRef]
  51. Quick, A.M.; Dockter, T.; Le-Rademacher, J.; Salani, R.; Hudson, C.; Hundley, A.; Terstriep, S.; Streicher, L.; Faubion, S.; Loprinzi, C.L.; et al. Pilot study of fractional CO2 laser therapy for genitourinary syndrome of menopause in gynecologic cancer survivors. Maturitas 2021, 144, 37–44. [Google Scholar] [CrossRef] [PubMed]
  52. Salvatore, S.; Nappi, R.E.; Casiraghi, A.; Ruffolo, A.F.; Degliuomini, R.; Parma, M.; Maggiore, U.L.R.; Athanasiou, S.; Candiani, M. Microablative fractional CO2 laser for vulvovaginal atrophy in women with a history of breast cancer: A pilot study at 4-week follow-up. Clin. Breast Cancer 2021, 21, e539–e546. [Google Scholar] [CrossRef] [PubMed]
Jcm 11 00774 g001 550
Figure 1. Consort diagram including long-term follow-up of study participants. GSM = Genitourinary syndrome of menopause.
Figure 1. Consort diagram including long-term follow-up of study participants. GSM = Genitourinary syndrome of menopause.
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Figure 2. Mean (diamond), median (white line), and the 1st and 3rd quartiles are displayed for the VAS and VuAS at baseline, 4 week, and 2 year follow-up. The whiskers are the distance equal to 1.5 times the interquartile range (IQR). The diagram also shows outliers (circles) which are values that above or below the whisker ends. VAS = Vaginal Assessment Scale; VuAS = Vulvar Assessment Scale.
Figure 2. Mean (diamond), median (white line), and the 1st and 3rd quartiles are displayed for the VAS and VuAS at baseline, 4 week, and 2 year follow-up. The whiskers are the distance equal to 1.5 times the interquartile range (IQR). The diagram also shows outliers (circles) which are values that above or below the whisker ends. VAS = Vaginal Assessment Scale; VuAS = Vulvar Assessment Scale.
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Jcm 11 00774 g003 550
Figure 3. Mean (diamond), median (white line), and the 1st and 3rd quartiles are displayed for the FSFI and FSDS at baseline, 4 week, and 1 and 2 year follow-ups. The whiskers are the distance equal to 1.5 times the interquartile range (IQR). The diagram also shows outliers (circles) which are values that above or below the whisker ends. FSFI = Female Sexual Function Index; FSDS = Female Sexual Distress Scale.
Figure 3. Mean (diamond), median (white line), and the 1st and 3rd quartiles are displayed for the FSFI and FSDS at baseline, 4 week, and 1 and 2 year follow-ups. The whiskers are the distance equal to 1.5 times the interquartile range (IQR). The diagram also shows outliers (circles) which are values that above or below the whisker ends. FSFI = Female Sexual Function Index; FSDS = Female Sexual Distress Scale.
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Jcm 11 00774 g004 550
Figure 4. Mean (diamond), median (white line), and the 1st and 3rd quartiles are displayed for the UDI at baseline, 4 week, and 1 and 2 year follow-ups. The whiskers are the distance equal to 1.5 times the interquartile range (IQR). The diagram also shows outliers (circles) which are values that above or below the whisker ends. UDI = Urinary Distress Inventory.
Figure 4. Mean (diamond), median (white line), and the 1st and 3rd quartiles are displayed for the UDI at baseline, 4 week, and 1 and 2 year follow-ups. The whiskers are the distance equal to 1.5 times the interquartile range (IQR). The diagram also shows outliers (circles) which are values that above or below the whisker ends. UDI = Urinary Distress Inventory.
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Table
Table 1. Participant and breast cancer treatment characteristics.
Table 1. Participant and breast cancer treatment characteristics.
Initial Participants with 4 Week Follow-Up (n = 64)Participants with 1 Year Follow-Up (n = 39)Participants with 2 Year Follow-Up (n = 33)
Age (on study)
Mean (SD)57.4 (9.5)57.7 (10.50)59.3 (10.8)
Stage
I32 (50.0%)19 (48.7%)15 (45.5%)
II21 (32.8%)13 (33.3%)10 (30.3%)
III7 (10.9%)4 (10.3%)5 (15.2%)
IV1 (1.6%) b1 (2.6%)0 (0%)
Histology
Adenocarcinoma51 (76.7%)32 (82.1%)27 (81.8%)
Other13 (20.3%)7 (18.0%)6 (18.2%)
ER status
Negative5 (7.8%)0 (0%)1 (3.0%)
Positive58 (90.6%)38 (97.4%)31 (93.9%)
Unknown1 (1.6%)1 (2.6%)1 (3.0%)
PR status
Negative15 (23.4%)6 (15.4%)7 (21.2%)
Positive47 (73.4%)31 (79.5%)25 (75.8%)
Unknown2 (3.1%)2 (5.1%)1 (3.0%)
HER2 Neu status
Negative50 (78.1%)28 (71.8%)26 (78.8%)
Positive11 (17.2%)9 (23.1%)4 (12.1%)
Unknown3 (4.7%)2 (5.1%)3 (9.1%)
ER−/PR−5 (7.8%)0 (0%)1 (3.0%)
ER+/PR+47 (73.4%)31 (79.5%)25 (75.8%)
ER+/PR−10 (15.6%)6 (15.4%)6 (18.2%)
Chemotherapy
Yes36 (56.3%) 20 (51.3%)16 (48.5%)
No27 (42.2%) 18 (46.2%)16 (48.5%)
Unknown1 (1.6%)1 (2.6%)1 (3.0%)
Endocrine therapy use
Yes59 (92.2%)37 (94.9%)32 (97.0%)
No4 (6.3%)1 (2.6%)0 (0%)
Unknown1 (1.6%)1 (2.6%)1 (3.0%)
Type of endocrine therapy
Tamoxifen13 (20.3%)9 (23.1%)7 (21.2%)
AI44 (68.8%)26 (66.7%)23 (69.7%)
Tamoxifen and ovarian suppression2 (3.1%)2 (5.1%)2 (6.1%)
SD = standard deviation; ER = estrogen receptor; PR = progesterone receptor, HER2 = human epidural growth factor receptor 2; AI = aromatase inhibitor.
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Quick, A.M.; Hundley, A.; Evans, C.; Stephens, J.A.; Ramaswamy, B.; Reinbolt, R.E.; Noonan, A.M.; Van Deusen, J.B.; Wesolowski, R.; Stover, D.G.; et al. Long-Term Follow-Up of Fractional CO2 Laser Therapy for Genitourinary Syndrome of Menopause in Breast Cancer Survivors. J. Clin. Med. 2022, 11, 774. https://doi.org/10.3390/jcm11030774

AMA Style

Quick AM, Hundley A, Evans C, Stephens JA, Ramaswamy B, Reinbolt RE, Noonan AM, Van Deusen JB, Wesolowski R, Stover DG, et al. Long-Term Follow-Up of Fractional CO2 Laser Therapy for Genitourinary Syndrome of Menopause in Breast Cancer Survivors. Journal of Clinical Medicine. 2022; 11(3):774. https://doi.org/10.3390/jcm11030774

Chicago/Turabian Style

Quick, Allison M., Andrew Hundley, Cynthia Evans, Julie A. Stephens, Bhuvaneswari Ramaswamy, Raquel E. Reinbolt, Anne M. Noonan, Jeffrey Bryan Van Deusen, Robert Wesolowski, Daniel G. Stover, and et al. 2022. "Long-Term Follow-Up of Fractional CO2 Laser Therapy for Genitourinary Syndrome of Menopause in Breast Cancer Survivors" Journal of Clinical Medicine 11, no. 3: 774. https://doi.org/10.3390/jcm11030774

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