Expression of p11 in Patients with Depression
Abstract
:1. Introduction
2. Materials and Methods
2.1. Characteristics of Study Participants
2.2. Molecular Analysis
2.2.1. Determination of Protein Concentration
2.2.2. Enzyme-Linked Immunosorbent Assay (ELISA)
2.2.3. Isolation of Total RNA
2.2.4. Quality Analysis of Isolated RNA
2.2.5. Reverse Transcription RT-PCR
2.2.6. Real-Time PCR Reaction—Scanning the miRNA Panel
2.3. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Analysed Trait | Statistical Parameters * | |||
---|---|---|---|---|
M | Me | SD | Min.–Max. | |
Number of hospitalizations 1 | 2.01 | 1 | 2.00 | 0–12 |
Disease duration time (years) | 6.18 | 4 | 7.05 | 1–40 |
Number of episodes | 4.56 | 2 | 5.33 | 1–20 |
HDRS * | 22.82 | 23 | 6.86 | 1–51 |
Gene | Study Group | Statistical Parameter | |||||||
---|---|---|---|---|---|---|---|---|---|
M | Me | Q1–Q3 (IQR) | SD | SE | 95% CI | Min.–Max. | p | ||
p11 protein [ng/mL] | Test group | 2.583 | 2.755 | 2.057–3.270 (1.213) | 0.781 | 0.057 | 2.471–2.695 | 0.690–3.940 | 0.2511 |
Control group | 2.384 | 2.464 | 2.069–2.780 (0.711) | 0.614 | 0.061 | 2.262–2.506 | 0.298–3.999 | ||
Overall | 2.514 | 2.560 | 2.060–3.160 (1.100) | 0.733 | 0.043 | 2.430–2.599 | 0.298–3.999 | ||
p11 (S100A10) mRNA [2−∆CT] | Test group | 0.669 | 0.722 | 0.534–0.835 (0.301) | 0.190 | 0.014 | 0.642–0.696 | 0.197–0.995 | 0.7794 |
Control group | 0.625 | 0.639 | 0.544–0.724 (0.180) | 0.140 | 0.014 | 0.597–0.652 | 0.158–0.884 | ||
Overall | 0.654 | 0.662 | 0.537–0.816 (0.279) | 0.175 | 0.010 | 0.633–0.674 | 0.158–0.995 |
p11 Protein | p11 mRNA | ||
---|---|---|---|
Number of hospitalizations | Correlation coefficient | 0.012 | 0.023 |
Sig. (2-tailed) | 0.8731 | 0.7508 | |
N | 186 | 186 | |
Disease duration (years) | Correlation coefficient | 0.030 | 0.046 |
Sig. (2-tailed) | 0.6795 | 0.5319 | |
N | 186 | 186 | |
Number of episodes | Correlation coefficient | 0.003 | 0.013 |
Sig. (2-tailed) | 0.9641 | 0.8557 | |
N | 186 | 186 | |
HDRS | Correlation coefficient | 0.058 | 0.051 |
Sig. (2-tailed) | 0.4369 | 0.4884 | |
N | 184 | 184 |
p11 Protein | p11 mRNA | ||
---|---|---|---|
Overall | Correlation coefficient | 0.124 * | 0.135 * |
Sig. (2-tailed) | 0.0350 | 0.0220 | |
N | 289 | 289 | |
Test group (depression) | Correlation coefficient | 0.080 | 0.092 |
Sig. (2-tailed) | 0.2729 | 0.2054 | |
N | 190 | 190 | |
Control group | Correlation coefficient | −0.162 | −0.138 |
Sig. (2-tailed) | 0.1085 | 0.1737 | |
N | 99 | 99 |
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Gałecka, M.; Bliźniewska-Kowalska, K.; Gałecki, P.; Szemraj, J.; Orzechowska, A. Expression of p11 in Patients with Depression. J. Clin. Med. 2022, 11, 5743. https://doi.org/10.3390/jcm11195743
Gałecka M, Bliźniewska-Kowalska K, Gałecki P, Szemraj J, Orzechowska A. Expression of p11 in Patients with Depression. Journal of Clinical Medicine. 2022; 11(19):5743. https://doi.org/10.3390/jcm11195743
Chicago/Turabian StyleGałecka, Małgorzata, Katarzyna Bliźniewska-Kowalska, Piotr Gałecki, Janusz Szemraj, and Agata Orzechowska. 2022. "Expression of p11 in Patients with Depression" Journal of Clinical Medicine 11, no. 19: 5743. https://doi.org/10.3390/jcm11195743
APA StyleGałecka, M., Bliźniewska-Kowalska, K., Gałecki, P., Szemraj, J., & Orzechowska, A. (2022). Expression of p11 in Patients with Depression. Journal of Clinical Medicine, 11(19), 5743. https://doi.org/10.3390/jcm11195743