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Article

Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis

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Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka St. 12, 20-950 Lublin, Poland
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Department of Biophysics, Faculty of Environmental Biology, University of Life Sciences in Lublin, Akademicka St. 13, 20-950 Lublin, Poland
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School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, Johannesburg 2193, South Africa
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Department of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Akademicka St. 19, 20-033 Lublin, Poland
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Bohdan Dobrzański Institute of Agrophysics of the Polish Academy of Sciences, Doświadczalna St. 4, 20-290 Lublin, Poland
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Chair and Department of Rehabilitation and Orthopaedics, Medical University in Lublin, Jaczewskiego St. 8, 20-090 Lublin, Poland
*
Authors to whom correspondence should be addressed.
Academic Editor: Amr El-Husseini
J. Clin. Med. 2021, 10(21), 4808; https://doi.org/10.3390/jcm10214808
Received: 15 September 2021 / Revised: 14 October 2021 / Accepted: 19 October 2021 / Published: 20 October 2021
(This article belongs to the Special Issue Secondary Osteoporosis and Metabolic Bone Diseases)
A metabolite of leucine, ß-hydroxy-ß-methylbutyrate (HMB), used as a dietary supplement effects muscle tissue gain and bone tissue quality. Since there are no studies on the effects of HMB during pregnancy yet, the aim of the current study was to determine the effects of HMB supplementation during pregnancy on osteoporotic bone quality postpartum and post-lactation using spiny mice (Acomys cahirinus) as the animal models. The six-month-old dams were divided into four groups: pregnant and lactating controls, and pregnant and lactating HMB-treated (during the second trimester of pregnancy) females. The intensity of the immunoreaction of osteocalcin (OC), osteoprotegerin (OPG), bone morphogenetic protein 2 (BMP-2), tissue inhibitor of metalloproteinases 2 (TIMP-2), matrix metalloproteinase 8 and 13 (MMP-8 and MMP-13) and proteins involved in bone turnover, was measured in femoral trabecular and compact bone, as well as in the hyaline and epiphyseal cartilage of the femora. The analysis of the trabecular bone microarchitecture showed that the administration of HMB to pregnant females, by influencing the proteins responsible for bone cell activity and collagen remodeling, can provide protection from bone loss. Based on the results of the current study it can be assumed that HMB administration to pregnant females has a more positive impact on trabecular than compact bone. View Full-Text
Keywords: β-hydroxy β-methylbutyrate; pregnancy; lactation; bone; bone quality; bone proteins β-hydroxy β-methylbutyrate; pregnancy; lactation; bone; bone quality; bone proteins
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MDPI and ACS Style

Tomaszewska, E.; Muszyński, S.; Donaldson, J.; Dobrowolski, P.; Chand, D.K.P.; Tomczyk-Warunek, A.; Hułas-Stasiak, M.; Puzio, I.; Lamorski, K.; Sławiński, C.; Jabłoński, M.; Blicharski, T. Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis. J. Clin. Med. 2021, 10, 4808. https://doi.org/10.3390/jcm10214808

AMA Style

Tomaszewska E, Muszyński S, Donaldson J, Dobrowolski P, Chand DKP, Tomczyk-Warunek A, Hułas-Stasiak M, Puzio I, Lamorski K, Sławiński C, Jabłoński M, Blicharski T. Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis. Journal of Clinical Medicine. 2021; 10(21):4808. https://doi.org/10.3390/jcm10214808

Chicago/Turabian Style

Tomaszewska, Ewa, Siemowit Muszyński, Janine Donaldson, Piotr Dobrowolski, Deepesh K.P. Chand, Agnieszka Tomczyk-Warunek, Monika Hułas-Stasiak, Iwona Puzio, Krzysztof Lamorski, Cezary Sławiński, Mirosław Jabłoński, and Tomasz Blicharski. 2021. "Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis" Journal of Clinical Medicine 10, no. 21: 4808. https://doi.org/10.3390/jcm10214808

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