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Article

Learning from Embryogenesis—A Comparative Expression Analysis in Melanoblast Differentiation and Tumorigenesis Reveals miRNAs Driving Melanoma Development

1
Institute of Biochemistry, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, Germany
2
Institute of Functional Genomics, University of Regensburg, 93053 Regensburg, Germany
3
Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, 1790 AB Den Burg, The Netherlands
4
Department of Biochemistry I, University of Regensburg, 93053 Regensburg, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Lionel Larribère
J. Clin. Med. 2021, 10(11), 2259; https://doi.org/10.3390/jcm10112259
Received: 13 April 2021 / Revised: 17 May 2021 / Accepted: 21 May 2021 / Published: 24 May 2021
(This article belongs to the Special Issue New Advances in Melanoma)
Malignant melanoma is one of the most dangerous tumor types due to its high metastasis rates and a steadily increasing incidence. During tumorigenesis, the molecular processes of embryonic development, exemplified by epithelial–mesenchymal transition (EMT), are often reactivated. For melanoma development, the exact molecular differences between melanoblasts, melanocytes, and melanoma cells are not completely understood. In this study, we aimed to identify microRNAs (miRNAs) that promote melanoma tumorigenesis and progression, based on an in vitro model of normal human epidermal melanocyte (NHEM) de-differentiation into melanoblast-like cells (MBrCs). Using miRNA-sequencing and differential expression analysis, we demonstrated in this study that a majority of miRNAs have an almost equal expression level in NHEMs and MBrCs but are significantly differentially regulated in primary tumor- and metastasis-derived melanoma cell lines. Further, a target gene analysis of strongly regulated but functionally unknown miRNAs yielded the implication of those miRNAs in many important cellular pathways driving malignancy. We hypothesize that many of the miRNAs discovered in our study are key drivers of melanoma development as they account for the tumorigenic potential that differentiates melanoma cells from proliferating or migrating embryonic cells. View Full-Text
Keywords: miRNAs; melanoma; embryogenesis; melanoblasts miRNAs; melanoma; embryogenesis; melanoblasts
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MDPI and ACS Style

Linck-Paulus, L.; Lämmerhirt, L.; Völler, D.; Meyer, K.; Engelmann, J.C.; Spang, R.; Eichner, N.; Meister, G.; Kuphal, S.; Bosserhoff, A.K. Learning from Embryogenesis—A Comparative Expression Analysis in Melanoblast Differentiation and Tumorigenesis Reveals miRNAs Driving Melanoma Development. J. Clin. Med. 2021, 10, 2259. https://doi.org/10.3390/jcm10112259

AMA Style

Linck-Paulus L, Lämmerhirt L, Völler D, Meyer K, Engelmann JC, Spang R, Eichner N, Meister G, Kuphal S, Bosserhoff AK. Learning from Embryogenesis—A Comparative Expression Analysis in Melanoblast Differentiation and Tumorigenesis Reveals miRNAs Driving Melanoma Development. Journal of Clinical Medicine. 2021; 10(11):2259. https://doi.org/10.3390/jcm10112259

Chicago/Turabian Style

Linck-Paulus, Lisa, Lisa Lämmerhirt, Daniel Völler, Katharina Meyer, Julia C. Engelmann, Rainer Spang, Norbert Eichner, Gunter Meister, Silke Kuphal, and Anja K. Bosserhoff. 2021. "Learning from Embryogenesis—A Comparative Expression Analysis in Melanoblast Differentiation and Tumorigenesis Reveals miRNAs Driving Melanoma Development" Journal of Clinical Medicine 10, no. 11: 2259. https://doi.org/10.3390/jcm10112259

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