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Open AccessArticle

Development and Characterization of an Enzyme Membrane Reactor for Fructo-Oligosaccharide Production

1
Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, 35390 Giessen, Germany
2
Faculty of Biology and Chemistry, Justus-Liebig University of Giessen, 35390 Giessen, Germany
3
Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Project Group Bioresources, Heinrich-Buff-Ring 26, 35392 Giessen, Germany
*
Author to whom correspondence should be addressed.
Membranes 2019, 9(11), 148; https://doi.org/10.3390/membranes9110148
Received: 15 October 2019 / Revised: 5 November 2019 / Accepted: 6 November 2019 / Published: 10 November 2019
(This article belongs to the Special Issue EWM 2019: Membranes for a Sustainable Future)
Fructo-oligosaccharides (FOS) are linear fructans comprising 2–5 fructose units linked to a terminal glucose residue. They are widely used as food and feed additives due to their sweetness, low calorific value, and prebiotic properties. Here we describe the synthesis of FOS catalyzed by a cell-free crude enzyme solution containing recombinant fructosyltransferase (1-FFT) produced in the yeast Kluyveromyces lactis. During the enzyme catalysis, glucose accumulates as a by-product and eventually inhibits FOS production. We therefore used an enzyme membrane reactor (EMR) to achieve the continuous removal of glucose and the simultaneous replenishment of sucrose. We observed a loss of flux during the reaction with the characteristics of complete pore blocking, probably caused by a combination of proteins (enzyme molecules) and polysaccharides (FOS). Such complex fouling mechanisms must be overcome to achieve the efficient production of FOS using EMR systems. View Full-Text
Keywords: ceramic membrane; enzyme membrane reactor (EMR); fructo-oligosaccharides (FOS); prebiotic nutrition; Kluyveromyces lactis; resistance-in-series model; flux modeling; complete pore blocking ceramic membrane; enzyme membrane reactor (EMR); fructo-oligosaccharides (FOS); prebiotic nutrition; Kluyveromyces lactis; resistance-in-series model; flux modeling; complete pore blocking
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Burghardt, J.P.; Coletta, L.A.; van der Bolt, R.; Ebrahimi, M.; Gerlach, D.; Czermak, P. Development and Characterization of an Enzyme Membrane Reactor for Fructo-Oligosaccharide Production. Membranes 2019, 9, 148.

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