Next Article in Journal
Membranes with Intrinsic Micro-Porosity: Structure, Solubility, and Applications
Previous Article in Journal
A Review on the Progress in Nanoparticle/C Hybrid CMS Membranes for Gas Separation
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Membranes 2019, 9(1), 1; https://doi.org/10.3390/membranes9010001

Comparative Analysis of Dry and Wet Porometry Methods for Characterization of Regular and Cross-Linked Virus Removal Filter Papers

1
Division for Nanotechnology and Functional Materials, Department for Engineering Sciences, Uppsala University, Box 534, SE-751 21 Uppsala, Sweden
2
Charles River Biopharmaceutical Services, Gottfried Hagen Str. 20, 51105 Köln, Germany
*
Author to whom correspondence should be addressed.
Both authors contributed equally to the work.
Received: 2 November 2018 / Revised: 2 December 2018 / Accepted: 14 December 2018 / Published: 20 December 2018
  |  
PDF [4614 KB, uploaded 20 December 2018]
  |  

Abstract

Pore-size distribution (PSD) is the most critical parameter for size-exclusion virus removal filters. Yet, different dry- and wet-state porometry methods yield different pore-size values. The goal of this work is to conduct comparative analysis of nitrogen gas sorption (NGSP), liquid-liquid and cryoporometry with differential scanning calorimetry (CP-DSC) methods with respect to characterization of regular and cross-linked virus removal filter paper based on cellulose nanofibers, i.e. the mille-feuille filter. The filters were further characterized with atomic force and scanning electron microscopy. Finally, the removal of the worst-case model virus, i.e. minute virus of mice (MVM; 20 nm, nonenveloped parvovirus) was evaluated. The results revealed that there is no difference of the obtained PSDs between the wet methods, i.e. DSC and liquid-liquid porometry (LLP), as well as no difference between the regular and cross-linked filters regardless of method. MVM filtration at different trans membrane pressure (TMP) revealed strong dependence of the virus removal capability on applied pressure. It was further observed that cross-linking filters showed enhanced virus removal, especially at lower TMP. In all, the results of this study highlight the complex nature of virus capture in size-exclusion filters. View Full-Text
Keywords: gas sorption porometry; liquid-liquid porometry; cryoporometry; mille-feuille filter, parvovirus; virus removal filter gas sorption porometry; liquid-liquid porometry; cryoporometry; mille-feuille filter, parvovirus; virus removal filter
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Gustafsson, S.; Westermann, F.; Hanrieder, T.; Jung, L.; Ruppach, H.; Mihranyan, A. Comparative Analysis of Dry and Wet Porometry Methods for Characterization of Regular and Cross-Linked Virus Removal Filter Papers. Membranes 2019, 9, 1.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Membranes EISSN 2077-0375 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top