Impact of the Membrane Structure of the Stationary Phase on Steric Exclusion Chromatography (SXC) of Lentiviral Vectors
Abstract
:1. Introduction
2. Materials and Methods
2.1. Lentiviral Vector Production, Harvest, and Clarification
2.2. Steric Exclusion Chromatography
2.2.1. Membrane and Housing
2.2.2. Chromatography Setup and Procedure
2.3. Analytics
2.3.1. LV Titer, Protein, and DNA Quantification
2.3.2. Lentiviral Vector Visualization
2.4. Statistical Analysis
3. Results and Discussion
3.1. Impact of Membrane Pore Size on the Separation Mechanism
3.2. Membrane Pore Size and PEG Buffer Concentration
3.3. Performance Comparison Using Different Membrane Layer Numbers
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Labisch, J.J.; Wiese, G.P.; Pflanz, K.; Linkhorst, J. Impact of the Membrane Structure of the Stationary Phase on Steric Exclusion Chromatography (SXC) of Lentiviral Vectors. Membranes 2023, 13, 849. https://doi.org/10.3390/membranes13100849
Labisch JJ, Wiese GP, Pflanz K, Linkhorst J. Impact of the Membrane Structure of the Stationary Phase on Steric Exclusion Chromatography (SXC) of Lentiviral Vectors. Membranes. 2023; 13(10):849. https://doi.org/10.3390/membranes13100849
Chicago/Turabian StyleLabisch, Jennifer J., G. Philip Wiese, Karl Pflanz, and John Linkhorst. 2023. "Impact of the Membrane Structure of the Stationary Phase on Steric Exclusion Chromatography (SXC) of Lentiviral Vectors" Membranes 13, no. 10: 849. https://doi.org/10.3390/membranes13100849