Next Article in Journal
Nickel (II) Preconcentration and Speciation Analysis During Transport from Aqueous Solutions Using a Hollow-fiber Permeation Liquid Membrane (HFPLM) Device
Next Article in Special Issue
Membrane Bioreactor Technology for the Development of Functional Materials from Sea-Food Processing Wastes and Their Potential Health Benefits
Previous Article in Journal
Electroformation of Giant Vesicles on a Polymer Mesh
Previous Article in Special Issue
Comparative Composition and Antioxidant Activity of Peptide Fractions Obtained by Ultrafiltration of Egg Yolk Protein Enzymatic Hydrolysates
Article Menu

Export Article

Open AccessArticle
Membranes 2011, 1(3), 195-216;

Monitoring Protein Fouling on Polymeric Membranes Using Ultrasonic Frequency-Domain Reflectometry

Department of Mechanical Engineering, University of Colorado at Boulder, Membrane Science, Engineering and Technology (MAST) Center, 427 UCB, Boulder, CO 80309–0427, USA
Genencor, a Danisco Division, 925 Page Mill Rd, Palo Alto, CA 94304, USA
Department of Civil, Environmental and Architectural Engineering, University of Colorado at Boulder, 428 UCB, Boulder, CO 80309–0428, USA
Author to whom correspondence should be addressed.
Received: 27 May 2011 / Revised: 26 July 2011 / Accepted: 5 August 2011 / Published: 10 August 2011
(This article belongs to the Special Issue Membrane Technology for Food and Bioprocessing Applications)
Full-Text   |   PDF [552 KB, uploaded 18 August 2011]


Novel signal-processing protocols were used to extend the in situ sensitivity of ultrasonic frequency-domain reflectometry (UFDR) for real-time monitoring of microfiltration (MF) membrane fouling during protein purification. Different commercial membrane materials, with a nominal pore size of 0.2 µm, were challenged using bovine serum albumin (BSA) and amylase as model proteins. Fouling induced by these proteins was observed in flat-sheet membrane filtration cells operating in a laminar cross-flow regime. The detection of membrane-associated proteins using UFDR was determined by applying rigorous statistical methodology to reflection spectra of ultrasonic signals obtained during membrane fouling. Data suggest that the total power reflected from membrane surfaces changes in response to protein fouling at concentrations as low as 14 μg/cm2, and results indicate that ultrasonic spectra can be leveraged to detect and monitor protein fouling on commercial MF membranes. View Full-Text
Keywords: proteins; microfiltration; fouling; ultrasonic frequency-domain reflectometry proteins; microfiltration; fouling; ultrasonic frequency-domain reflectometry
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Kujundzic, E.; Greenberg, A.R.; Fong, R.; Hernandez, M. Monitoring Protein Fouling on Polymeric Membranes Using Ultrasonic Frequency-Domain Reflectometry. Membranes 2011, 1, 195-216.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Membranes EISSN 2077-0375 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top