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Article

Induction of Protection in Mice against a Chlamydia muridarum Respiratory Challenge by a Vaccine Formulated with the Major Outer Membrane Protein in Nanolipoprotein Particles

1
Department of Pathology and Laboratory Medicine, University of California, Irvine, CA 92697, USA
2
Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94551, USA
3
School of Medicine, Radiation Oncology, University of California Davis, Sacramento, CA 95616, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Ralph A. Tripp
Vaccines 2021, 9(7), 755; https://doi.org/10.3390/vaccines9070755
Received: 26 April 2021 / Revised: 18 June 2021 / Accepted: 22 June 2021 / Published: 7 July 2021
(This article belongs to the Section Vaccines against Infectious Diseases)
Chlamydia trachomatis is a sexually transmitted bacterium that infects over 130 million individuals worldwide annually. To implement a vaccine, we developed a cell-free co-translational system to express the Chlamydia muridarum major outer membrane protein (MOMP). This approach uses a nanolipoprotein particles (tNLP) made from ApoA1 protein, amphiphilic telodendrimer and lipids that self-assemble to form 10–25 nm discs. These tNLP provide a protein-encapsulated lipid support to solubilize and fold membrane proteins. The cell-free system co-translated MOMP and ApoA1 in the presence of telodendrimer mixed with lipids. The MOMP-tNLP complex was amenable to CpG and FSL-1 adjuvant addition. To investigate the ability of MOMP-tNLP+CpG+FSL-1 to induce protection against an intranasal (i.n.) C. muridarum challenge, female mice were vaccinated intramuscularly (i.m.) or i.n. and i.m. simultaneously 4 weeks apart. Following vaccination with MOMP-tNLP+CpG+FSL-1, mice mounted significant humoral and cell-mediated immune responses. Following the i.n. challenge, mice vaccinated with MOMP-tNLP+CpG+FSL-1 i.n. + i.m. group were protected as determined by the percentage change in body weight and by the number of C. muridarum inclusion forming units (IFU) recovered from the lungs. To our knowledge, this is the first time a MOMP-based vaccine formulated in tNLP has been shown to protect against C. muridarum. View Full-Text
Keywords: Chlamydia; vaccine; immunization; mice; nanolipoprotein particles; major outer membrane protein; intranasal challenge; telodisks; amphipols Chlamydia; vaccine; immunization; mice; nanolipoprotein particles; major outer membrane protein; intranasal challenge; telodisks; amphipols
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MDPI and ACS Style

Tifrea, D.F.; He, W.; Pal, S.; Evans, A.C.; Gilmore, S.F.; Fischer, N.O.; Rasley, A.; Coleman, M.A.; de la Maza, L.M. Induction of Protection in Mice against a Chlamydia muridarum Respiratory Challenge by a Vaccine Formulated with the Major Outer Membrane Protein in Nanolipoprotein Particles. Vaccines 2021, 9, 755. https://doi.org/10.3390/vaccines9070755

AMA Style

Tifrea DF, He W, Pal S, Evans AC, Gilmore SF, Fischer NO, Rasley A, Coleman MA, de la Maza LM. Induction of Protection in Mice against a Chlamydia muridarum Respiratory Challenge by a Vaccine Formulated with the Major Outer Membrane Protein in Nanolipoprotein Particles. Vaccines. 2021; 9(7):755. https://doi.org/10.3390/vaccines9070755

Chicago/Turabian Style

Tifrea, Delia F., Wei He, Sukumar Pal, Angela C. Evans, Sean F. Gilmore, Nicholas O. Fischer, Amy Rasley, Matthew A. Coleman, and Luis M. de la Maza 2021. "Induction of Protection in Mice against a Chlamydia muridarum Respiratory Challenge by a Vaccine Formulated with the Major Outer Membrane Protein in Nanolipoprotein Particles" Vaccines 9, no. 7: 755. https://doi.org/10.3390/vaccines9070755

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