Passive Immunization with Recombinant Antibody VLRB-PirAvp/PirBvp—Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp
Abstract
:1. Introduction
2. Materials and Methods
2.1. Construction of Toxin Plasmids
2.2. Expression and Purification of Recombinant Toxins
2.3. Screening of VLRB Library
2.4. Establishment of Cell Line Secreting Anti-PirAvp/PirBvp Recombinant VLRBs
2.5. Bacterial Challenge Test
2.6. Statistical Analysis
3. Results
3.1. Expression of Recombinant Toxins
3.2. Specificity of Established Toxin-Specific VLRBs
3.3. Protective Efficiency of Toxin-Specific VLRBs in Shrimp Infected with V. parahaemolyticus
4. Discussions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Sequence (5′-3′) | Reference/Accession No. |
---|---|---|
For PirABvp amplification | ||
p32-ToxA/Nco1-Fwd | TATACCATGGAGTAACAATATAAAACATG | AB972427.1 |
p32-ToxA/Sac1-Rev | GATGAGCTCTTAGTGGTAATAGATTGTAC | AB972427.1 |
p2b-ToxB/Nco1-Fwd: | ATACCATGGGCATGACTAACGAATACGTTGTAACAATGTC | AB972427.1 |
p2b-ToxB-TStrep/STOP/Sac1/Rev | AGCGAGCTCTCACTTTTCAAACTGCGGATGGCTCCACGCGCTGCCACCGCTACCGCCACCGCTACCGCCACCTTTCTCAAACTGTGGATGGCT | AB972427.1 |
For cell line | ||
LRRNT Sfi I forward | AGGCCACCGGGGCCTGTCCTTCACGGTGTTCCTG | [19] |
Stalk Sfi I reverse | TGGCCCAGAGGCCCGCGTTCATGACACGGCCGA | [19] |
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Lazarte, J.M.S.; Kim, Y.R.; Lee, J.S.; Chun, J.H.; Kim, S.W.; Jung, J.W.; Kim, J.; Kayansamruaj, P.; Thompson, K.D.; Kim, H.; et al. Passive Immunization with Recombinant Antibody VLRB-PirAvp/PirBvp—Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp. Vaccines 2021, 9, 55. https://doi.org/10.3390/vaccines9010055
Lazarte JMS, Kim YR, Lee JS, Chun JH, Kim SW, Jung JW, Kim J, Kayansamruaj P, Thompson KD, Kim H, et al. Passive Immunization with Recombinant Antibody VLRB-PirAvp/PirBvp—Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp. Vaccines. 2021; 9(1):55. https://doi.org/10.3390/vaccines9010055
Chicago/Turabian StyleLazarte, Jassy Mary S., Young Rim Kim, Jung Seok Lee, Jin Hong Chun, Si Won Kim, Jae Wook Jung, Jaesung Kim, Pattanapon Kayansamruaj, Kim D. Thompson, Hyeongsu Kim, and et al. 2021. "Passive Immunization with Recombinant Antibody VLRB-PirAvp/PirBvp—Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp" Vaccines 9, no. 1: 55. https://doi.org/10.3390/vaccines9010055