Salmonella Vaccine Vector System for Foot-and-Mouth Disease Virus and Evaluation of Its Efficacy with Virus-Like Particles
1
Research Division for Radiation Science, Korea Atomic Energy Research Institute, Jeongeup 56212, Korea
2
Department of Radiation Science, University of Science and Technology, Daejeon 34113, Korea
3
Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 08826, Korea
4
State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China
5
Department of Microbiology, Ewha Womans University College of Medicine, Seoul 07804, Korea
6
Ewha Education & Research Center for Infection, Ewha Womans University Medical Center, Seoul 07985, Korea
7
Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Korea
*
Authors to whom correspondence should be addressed.
Vaccines 2021, 9(1), 22; https://doi.org/10.3390/vaccines9010022
Received: 15 December 2020
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Revised: 29 December 2020
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Accepted: 31 December 2020
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Published: 5 January 2021
(This article belongs to the Special Issue Vaccine Evaluation Methods and Studies)
Foot-and-mouth disease virus (FMDV) causes a highly contagious and devastating disease in livestock animals and has a great potential to cause severe economic loss worldwide. The major antigen of FMDV capsid protein, VP1, contains the major B-cell epitope responsible for effectively eliciting protective humoral immunity. In this study, irradiated Salmonella Typhimurium (KST0666) were used as transgenic vectors containing stress-inducible plasmid pRECN-VP1 to deliver the VP1 protein from FMDV-type A/WH/CHA/09. Mice were orally inoculated with ATOMASal-L3 harboring pRECN-VP1, and FMDV virus-like particles, where (VLPFMDV)-specific humoral, mucosal, and cellular immune responses were evaluated. Mice vaccinated with attenuated Salmonella (KST0666) expressing VP1 (named KST0669) showed high levels of VLP-specific IgA in feces and IgG in serum, with high FMDV neutralization titer. Moreover, KST0669-vaccinated mice showed increased population of IFN-γ (type 1 T helper cells; Th1 cells)-, IL-5 (Th2 cells)-, and IL-17A (Th17 cells)-expressing CD4+ as well as activated CD8+ T cells (IFN-γ+CD8+ cells), detected by stimulating VLPFMDV. All data indicate that our Salmonella vector system successfully delivered FMDV VP1 to immune cells and that the humoral and cellular efficacy of the vaccine can be easily evaluated using VLPFMDV in a Biosafety Level I (BSL1) laboratory.
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Keywords:
foot-and-mouth disease; VP1; live attenuated Salmonella vector; mucosal immunity; virus-like particle; radiation mutation
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MDPI and ACS Style
Zhi, Y.; Ji, H.J.; Guo, H.; Lim, J.H.; Byun, E.-B.; Kim, W.S.; Seo, H.S. Salmonella Vaccine Vector System for Foot-and-Mouth Disease Virus and Evaluation of Its Efficacy with Virus-Like Particles. Vaccines 2021, 9, 22. https://doi.org/10.3390/vaccines9010022
AMA Style
Zhi Y, Ji HJ, Guo H, Lim JH, Byun E-B, Kim WS, Seo HS. Salmonella Vaccine Vector System for Foot-and-Mouth Disease Virus and Evaluation of Its Efficacy with Virus-Like Particles. Vaccines. 2021; 9(1):22. https://doi.org/10.3390/vaccines9010022
Chicago/Turabian StyleZhi, Yong, Hyun Jung Ji, Huichen Guo, Jae Hyang Lim, Eui-Baek Byun, Woo Sik Kim, and Ho Seong Seo. 2021. "Salmonella Vaccine Vector System for Foot-and-Mouth Disease Virus and Evaluation of Its Efficacy with Virus-Like Particles" Vaccines 9, no. 1: 22. https://doi.org/10.3390/vaccines9010022
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