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Article

Targeted Modification of the Foot-And-Mouth Disease Virus Genome for Quick Cell Culture Adaptation

1
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany
2
Merck KGaA, Merck Life Sciences, Upstream R&D, 64293 Darmstadt, Germany
*
Author to whom correspondence should be addressed.
Vaccines 2020, 8(4), 583; https://doi.org/10.3390/vaccines8040583
Received: 29 August 2020 / Revised: 19 September 2020 / Accepted: 30 September 2020 / Published: 3 October 2020
(This article belongs to the Special Issue Foot and Mouth Disease Virus and Novel Vaccines Developments)
Foot-and-mouth disease virus (FMDV) causes the highly contagious foot-and-mouth disease, which is characterized by the appearance of vesicles in and around the mouth and feet of cloven-hoofed animals. BHK-21 cells are the cell line of choice for the propagation of FMDV for vaccine production worldwide but vary in their susceptibility for different FMDV strains. Previous studies showed that the FMDV resistance of a certain BHK cell line can be overcome by using a closely related but permissive cell line for the pre-adaptation of the virus, but the adapted strains were found to harbor several capsid mutations. In this study, these adaptive mutations were introduced into the original Asia-1 Shamir isolate individually or in combination to create a panel of 17 Asia-1 mutants by reverse genetics and examine the effects of the mutations on receptor usage, viral growth, immunogenicity and stability. A single amino acid exchange from glutamic acid to lysine at position 202 in VP1 turned out to be of major importance for productive infection of the suspension cell line BHK-2P. In consequence, two traditionally passage-derived strains and two recombinant viruses with a minimum set of mutations were tested in vivo. While the passaged-derived viruses showed a reduced particle stability, the genetically modified viruses were more stable but did not confer a protective immune response against the original virus isolate. View Full-Text
Keywords: foot-and-mouth disease virus; serotype Asia-1; BHK suspension cells; mutagenesis; particle stability; neutralizing antibody response; recombinant virus, vaccine production foot-and-mouth disease virus; serotype Asia-1; BHK suspension cells; mutagenesis; particle stability; neutralizing antibody response; recombinant virus, vaccine production
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MDPI and ACS Style

Dill, V.; Zimmer, A.; Beer, M.; Eschbaumer, M. Targeted Modification of the Foot-And-Mouth Disease Virus Genome for Quick Cell Culture Adaptation. Vaccines 2020, 8, 583. https://doi.org/10.3390/vaccines8040583

AMA Style

Dill V, Zimmer A, Beer M, Eschbaumer M. Targeted Modification of the Foot-And-Mouth Disease Virus Genome for Quick Cell Culture Adaptation. Vaccines. 2020; 8(4):583. https://doi.org/10.3390/vaccines8040583

Chicago/Turabian Style

Dill, Veronika, Aline Zimmer, Martin Beer, and Michael Eschbaumer. 2020. "Targeted Modification of the Foot-And-Mouth Disease Virus Genome for Quick Cell Culture Adaptation" Vaccines 8, no. 4: 583. https://doi.org/10.3390/vaccines8040583

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