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Characteristics of a Novel Target Antigen against Myeloma Cells for Immunotherapy

Division of Clinical Physiology and Therapeutics, Faculty of Pharmacy, Keio University, Tokyo 105-8512, Japan
Author to whom correspondence should be addressed.
Vaccines 2020, 8(4), 579;
Received: 27 August 2020 / Revised: 20 September 2020 / Accepted: 28 September 2020 / Published: 2 October 2020
(This article belongs to the Special Issue Cancer Immunotherapy: Advances and Future Prospects)
Despite the availability of therapeutic treatments, multiple myeloma is an incurable haematological disorder. In this study, we aimed to clarify the role of CXorf48 as a therapeutic target in multiple myeloma. Based on a previously identified HLA-A*24:02-restiricted epitope from this novel cancer/testis antigen, we characterized the activities of cytotoxic T lymphocytes (CTLs) specific to this antigen against myeloma cells and evaluated the effects of demethylating agents in increasing antigen expression and enhancing the cytotoxic activity of CTLs. CXorf48 expression was examined by reverse transcription polymerase chain reaction (RT-PCR) using nine myeloma cell lines. Cell lines with low CXorf48 expression were treated by demethylating agents (DMAs), 5-azacytidine (5-aza), and 5-aza-2’-deoxycytidine (DAC) to evaluate gene expression using quantitative RT-PCR. Furthermore, CXorf48-specific CTLs were induced from peripheral blood mononuclear cells of HLA-A*24:02-positive healthy donors to evaluate antigen recognition using ELISpot and 51Cr cytotoxicity assays. CXorf48 was widely expressed in myeloma cells, and gene expression was significantly increased by DMAs. Furthermore, CXorf48-specific CTLs recognized DMA-treated myeloma cells. These findings suggest that CXorf48 is a useful target for immunotherapy, such as vaccination, in combination with demethylating agents for the treatment of patients with myeloma. View Full-Text
Keywords: myeloma; antigen; demethylating agents myeloma; antigen; demethylating agents
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MDPI and ACS Style

Matsushita, M.; Saito, S.; Yokoe, S.; Ichikawa, D.; Hattori, Y. Characteristics of a Novel Target Antigen against Myeloma Cells for Immunotherapy. Vaccines 2020, 8, 579.

AMA Style

Matsushita M, Saito S, Yokoe S, Ichikawa D, Hattori Y. Characteristics of a Novel Target Antigen against Myeloma Cells for Immunotherapy. Vaccines. 2020; 8(4):579.

Chicago/Turabian Style

Matsushita, Maiko, Saku Saito, Shinya Yokoe, Daiju Ichikawa, and Yutaka Hattori. 2020. "Characteristics of a Novel Target Antigen against Myeloma Cells for Immunotherapy" Vaccines 8, no. 4: 579.

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