Next Article in Journal
Infections, Vaccines and Autoimmunity: A Multiple Sclerosis Perspective
Next Article in Special Issue
Development of Tumor Cell-Based Vaccine with IL-12 Gene Electrotransfer as Adjuvant
Previous Article in Journal
Mechanisms of Mixed Th1/Th2 Responses in Mice Induced by Albizia julibrissin Saponin Active Fraction by in Silico Analysis
Previous Article in Special Issue
Modulation of Vaccine-Induced HIV-1-Specific Immune Responses by Co-Electroporation of PD-L1 Encoding DNA
Article

Electrotransfer of siRNA to Silence Enhanced Green Fluorescent Protein in Tumor Mediated by a High Intensity Pulsed Electromagnetic Field

1
Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloška 2, 1000 Ljubljana, Slovenia
2
Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000 Ljubljana, Slovenia
3
Faculty of Health Sciences, University of Primorska, Polje 42, 6310 Izola, Slovenia
4
Department of Cytopathology, Institute of Oncology Ljubljana, Zaloška 2, 1000 Ljubljana, Slovenia
5
Faculty of Health Sciences, University of Ljubljana, Zdravstvena pot 5, 1000 Ljubljana, Slovenia
*
Author to whom correspondence should be addressed.
Vaccines 2020, 8(1), 49; https://doi.org/10.3390/vaccines8010049
Received: 12 December 2019 / Revised: 10 January 2020 / Accepted: 23 January 2020 / Published: 27 January 2020
(This article belongs to the Special Issue Immunization by Electroporation)
The contactless high intensity pulsed electromagnetic field (HI-PEMF)-induced increase of cell membrane permeability is similar to conventional electroporation, with the important difference of inducing an electric field non-invasively by exposing a treated tissue to a time-varying magnetic field. Due to the limited number of studies in the field of electroporation induced by HI-PEMF, we designed experiments to explore the feasibility of such a contactless delivery technique for the gene electrotransfer of nucleic acids in tissues in vivo. By using HI-PEMF for gene electrotransfer, we silenced enhanced green fluorescent protein (EGFP) with siRNA molecules against EGFP in B16F10-EGFP tumors. Six days after the transfer, the fluorescent tumor area decreased by up to 39% as determined by fluorescence imaging in vivo. In addition, the silencing of EGFP to the same extent was confirmed at the mRNA and protein level. The results obtained in the in vivo mouse model demonstrate the potential use of HI-PEMF-induced cell permeabilization for gene therapy and DNA vaccination. Further studies are thus warranted to improve the equipment, optimize the protocols for gene transfer and the HI-PEMF parameters, and demonstrate the effects of HI-PEMF on a broader range of different normal and tumor tissues. View Full-Text
Keywords: high intensity pulsed electromagnetic field; electrotransfer; enhanced green fluorescent protein; siRNA; mouse melanoma model; tumor high intensity pulsed electromagnetic field; electrotransfer; enhanced green fluorescent protein; siRNA; mouse melanoma model; tumor
Show Figures

Figure 1

MDPI and ACS Style

Kranjc Brezar, S.; Kranjc, M.; Čemažar, M.; Buček, S.; Serša, G.; Miklavčič, D. Electrotransfer of siRNA to Silence Enhanced Green Fluorescent Protein in Tumor Mediated by a High Intensity Pulsed Electromagnetic Field. Vaccines 2020, 8, 49. https://doi.org/10.3390/vaccines8010049

AMA Style

Kranjc Brezar S, Kranjc M, Čemažar M, Buček S, Serša G, Miklavčič D. Electrotransfer of siRNA to Silence Enhanced Green Fluorescent Protein in Tumor Mediated by a High Intensity Pulsed Electromagnetic Field. Vaccines. 2020; 8(1):49. https://doi.org/10.3390/vaccines8010049

Chicago/Turabian Style

Kranjc Brezar, Simona, Matej Kranjc, Maja Čemažar, Simon Buček, Gregor Serša, and Damijan Miklavčič. 2020. "Electrotransfer of siRNA to Silence Enhanced Green Fluorescent Protein in Tumor Mediated by a High Intensity Pulsed Electromagnetic Field" Vaccines 8, no. 1: 49. https://doi.org/10.3390/vaccines8010049

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop