Simple Nanoparticles from the Assembly of Cationic Polymer and Antigen as Immunoadjuvants
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Stock Solutions
2.2. Preparation of the PDDA/OVA Nanoparticles (NPs)
2.3. Determination of Physical Properties and Colloidal Stability for NPs Dispersions from Dynamic Light Scattering (DLS), Turbidimetry, and Scanning Electron Microscopy (SEM)
2.4. Cell Culture, Preparation of PDDA, OVA, or PDDA/OVA Solutions for Interaction with Cells, and Determination of Cell Viability in the Interaction Mixtures
2.5. Determination of Cells Morphology in the Presence of PDDA by Scanning Electron Microscopy (SEM)
2.6. Immunization Scheme
2.7. Determination of OVA-Specific IgG1 and IgG2a Antibodies
2.8. Determination of Delayed-Type Hypersensitivity Reaction (DTH)
2.9. Statistical Analysis
3. Results and Discussion
3.1. Characterization of PDDA/OVA Dispersions Regarding Formation of Nanoparticles, Their Size, Surface Potential, Polydispersity, Morphology, and Colloidal Stability.
3.2. Cytotoxicity of PDDA/OVA NPs Against Mammalian Cells in Culture
3.3. Immunoadjuvant Properties of PDDA/OVA NPs.
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Group | Solution or Dispersion | [OVA] mg·mL−1 | [Al(OH)3] mg·mL−1 | [PDDA] mg·mL−1 |
---|---|---|---|---|
1 | Milli-Q water | 0 | 0 | 0 |
2 | OVA | 0.1 | 0 | 0 |
3 | Al(OH)3/OVA | 0.1 | 0.1 | 0 |
4 | PDDA/OVA | 0.1 | 0 | 0.01 |
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Pérez-Betancourt, Y.; Távora, B.d.C.L.F.; Colombini, M.; Faquim-Mauro, E.L.; Carmona-Ribeiro, A.M. Simple Nanoparticles from the Assembly of Cationic Polymer and Antigen as Immunoadjuvants. Vaccines 2020, 8, 105. https://doi.org/10.3390/vaccines8010105
Pérez-Betancourt Y, Távora BdCLF, Colombini M, Faquim-Mauro EL, Carmona-Ribeiro AM. Simple Nanoparticles from the Assembly of Cationic Polymer and Antigen as Immunoadjuvants. Vaccines. 2020; 8(1):105. https://doi.org/10.3390/vaccines8010105
Chicago/Turabian StylePérez-Betancourt, Yunys, Bianca de Carvalho Lins Fernandes Távora, Mônica Colombini, Eliana L. Faquim-Mauro, and Ana Maria Carmona-Ribeiro. 2020. "Simple Nanoparticles from the Assembly of Cationic Polymer and Antigen as Immunoadjuvants" Vaccines 8, no. 1: 105. https://doi.org/10.3390/vaccines8010105