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Open AccessArticle

Maternally-Derived Antibodies Protect against Challenge with Highly Pathogenic Avian Influenza Virus of the H7N3 Subtype

1
Department of Population Health, University of Georgia, Poultry Diagnostic and Research Center, Athens, GA 30602, USA
2
Southern Poultry Research Group, Watkinsville, GA 30677, USA
*
Authors to whom correspondence should be addressed.
Current affiliation: Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Current affiliation: Department of Microbiology, University of Washington, Seattle, WA 98195, USA.
Vaccines 2019, 7(4), 163; https://doi.org/10.3390/vaccines7040163
Received: 18 September 2019 / Revised: 27 October 2019 / Accepted: 28 October 2019 / Published: 30 October 2019
(This article belongs to the Section Influenza Virus Vaccines)
Vaccination of hens against influenza leads to the transfer of protective maternally-derived antibodies (MDA) to hatchlings. However, little is known about the transfer of H7N3 vaccine-induced MDA. Here, we evaluated transfer, duration, and protective effect of MDA in chickens against H7N3 HPAIV. To generate chickens with MDA (MDA (+)), 15-week-old White Leghorn hens were vaccinated and boosted twice with an inactivated H7N3 low pathogenic avian influenza virus vaccine, adjuvanted with Montanide ISA 71 VG. One week after the final boost, eggs were hatched. Eggs from non-vaccinated hens were hatched for chickens without MDA (MDA (−)). Both MDA (+) and MDA (−) hatchlings were monitored weekly for antibody levels. Anti-HA MDA were detected by hemagglutination inhibition assay mostly until day 7 post-hatch. However, anti-nucleoprotein MDA were still detected three weeks post-hatch. Three weeks post-hatch, chickens were challenged with 106 EID50/bird of Mexican-origin H7N3 HPAIV. Interestingly, while 0% of the MDA (−) chickens survived the challenge, 95% of the MDA (+) chickens survived. Furthermore, virus shedding was significantly reduced by day 5 post-challenge in the MDA (+) group. In conclusion, MDA confers partial protection against mortality upon challenge with H7N3 HPAIV, as far as three weeks post-hatch, even in the absence of detectable anti-HA antibodies, and reduce virus shedding after challenge. View Full-Text
Keywords: maternally-derived antibodies; highly pathogenic avian influenza virus; H7N3; vaccination of chickens; protection maternally-derived antibodies; highly pathogenic avian influenza virus; H7N3; vaccination of chickens; protection
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Cardenas-Garcia, S.; Ferreri, L.; Wan, Z.; Carnaccini, S.; Geiger, G.; Obadan, A.O.; Hofacre, C.L.; Rajao, D.; Perez, D.R. Maternally-Derived Antibodies Protect against Challenge with Highly Pathogenic Avian Influenza Virus of the H7N3 Subtype. Vaccines 2019, 7, 163.

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