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Open AccessArticle

Sea Bass Immunization to Downsize the Betanodavirus Protein Displayed in the Surface of Inactivated Repair-Less Bacteria

1
Institute of Marine Research (IIM), Spanish National Research Council (CSIC), Eduardo Cabello 6, 36208 Vigo, Spain
2
Biotechnology Department, National Institute for Agricultural and Food Research and Technology (INIA), La Coruña road, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Vaccines 2019, 7(3), 94; https://doi.org/10.3390/vaccines7030094
Received: 24 July 2019 / Revised: 15 August 2019 / Accepted: 16 August 2019 / Published: 20 August 2019
(This article belongs to the Section Veterinary Vaccines)
This work describes immunization of European sea bass (Dicentrarchus labrax) juveniles against viral nervous necrosis virus (VNNV), a betanodavirus causing worldwide mortalities in many fish species. Protection was obtained with the so-called spinycterin vehicles consisting of irreversibly DNA-damaged DNA-repair-less Escherichia coli displaying at their surface a downsized VNNV coat antigen. In this work we have (i) maximized bacterial expression levels by downsizing the coat protein of VNNV to a fragment (frgC91–220) containing most of its previously determined antigenicity, (ii) developed a scalable autoinduction culture media for E. coli based in soy-bean rather than in casein hydrolysates, (iii) enriched surface expression by screening different anchors from several prokaryotic sources (anchor + frgC91–220 recombinant products), (iv) preserved frgC91–220 antigenicity by inactivating bacteria by irreversible DNA-damage by means of Ciprofloxacin, and (v) increased safety using a repair-less E. coli strain as chassis for the spinycterins. These spinycterins protected fish against VNNV challenge with partial (Nmistic + frgC91–220) or total (YBEL + frgC91–220) levels of protection, in contrast to fish immunized with frgC91–220 spinycterins. The proposed spinycterin platform has high levels of environmental safety and cost effectiveness and required no adjuvants, thus providing potential to further develop VNNV vaccines for sustainable aquaculture. View Full-Text
Keywords: VNNV; mass-immunization; sea bass; recombinant bacterins; spinycterins; DNA-damaged; repair-less VNNV; mass-immunization; sea bass; recombinant bacterins; spinycterins; DNA-damaged; repair-less
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MDPI and ACS Style

Lama, R.; Pereiro, P.; Novoa, B.; Coll, J. Sea Bass Immunization to Downsize the Betanodavirus Protein Displayed in the Surface of Inactivated Repair-Less Bacteria. Vaccines 2019, 7, 94.

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